NMR paper Sarcolemma phospholipid structure investigated by immunogold electron microscopy and

		BioNMR > Educational resources > Journal club
[NMR paper] Sarcolemma phospholipid structure investigated by immunogold electron microscopy and

Webservers

NMR processing:

MDD

NMR assignment:

Backbone:

Side-chains:

NOEs:

Structure from NMR restraints:

Ab initio:

Fragment-based:

Rosetta-NMR (Robetta)

Template-based:

GeNMR

I-TASSER

Refinement:

Amber

Structure from chemical shifts:

Fragment-based:

Homology-based:

Torsion angles from chemical shifts:

Preditor

TALOS

Promega- Proline

Secondary structure from chemical shifts:

CSI (via RCI server)

TALOS

MICS caps, β-turns

d2D

PECAN

Flexibility from chemical shifts:

RCI

Interactions from chemical shifts:

HADDOCK

Chemical shifts re-referencing:

NMR model quality:

NOEs, other restraints:

Chemical shifts:

RDCs:

Pseudocontact shifts:

Protein geomtery:

SAVES2 or SAVES4

Quality Control Check

NMR spectrum prediction:

FANDAS

MestReS

V-NMR

Flexibility from structure:

Backbone S2

Methyl S2

B-factor

Molecular dynamics:

Gromacs

Amber

Antechamber

Chemical shifts prediction:

From structure:

Shiftx2

Sparta+

Camshift

CH3shift- Methyl

ArShift- Aromatic

ShiftS

Proshift

PPM

CheShift-2- Cα

From sequence:

Shifty

Camcoil

Poulsen_rc_CS

Disordered proteins:

MAXOCC

Format conversion & validation:

CCPN

From NMR-STAR 3.1

Validate NMR-STAR 3.1

NMR sample preparation:

Protein disorder:

DisMeta

Protein solubility:

Isotope labeling:

Solid-state NMR:

Thread Tools

Search this Thread

Rate Thread

Display Modes

11-19-2010, 08:44 PM

nmrlearner

Senior Member

Join Date: Jan 2005

Posts: 23,732

Points: 193,617, Level: 100

Level up: 0%, 0 Points needed

Activity: 50.7%

Last Achievements

Award-Showcase

NMR Credits: 0

NMR Points: 193,617

Downloads: 0

Uploads: 0

Sarcolemma phospholipid structure investigated by immunogold electron microscopy and

Sarcolemma phospholipid structure investigated by immunogold electron microscopy and (31)P NMR spectroscopy with lanthanide ions.

Related Articles Sarcolemma phospholipid structure investigated by immunogold electron microscopy and (31)P NMR spectroscopy with lanthanide ions.

FEBS Lett. 2001 Dec 14;509(3):417-22

Authors: Moreau C, Cavalier A, Le Floch M, Segalen J, Rocher C, Traïkia M, Leray G, Bondon A, Thomas D, Le Rumeur E

The biological functions of plasma membranes depend greatly on the biophysical properties resulting from protein and phospholipid structure. We investigated the phospholipid structure of the normal sarcolemma membrane, which is known to be highly dysfunctional in myopathies. Combining electron microscopy and (31)P nuclear magnetic resonance (NMR) spectroscopy on isolated sarcolemma vesicles, we find that (i) the sarcolemma vesicles maintain the in-vivo cellular sidedness, (ii) the phospholipid mobility is close to that observed in model membranes (similar lateral diffusion coefficients and spin-lattice T(1) relaxation times). Using broad-band and magic angle spinning (31)P NMR spectroscopy with lanthanide ions (Pr(3+)), it is possible to quantify the distribution of phospholipids between internal and external membrane layers, showing that the trans-bilayer distribution is highly asymmetrical.

PMID: 11749966 [PubMed - indexed for MEDLINE]

Source: PubMed

Did you find this post helpful?

Similar Threads
Thread	Thread Starter	Forum	Replies	Last Post
Effect of Freezing Conditions on Distances and Their Distributions Derived from Double Electron Electron Resonance (DEER): A Study of Doubly-Spin-Labeled T4 Lysozyme Effect of Freezing Conditions on Distances and Their Distributions Derived from Double Electron Electron Resonance (DEER): A Study of Doubly-Spin-Labeled T4 Lysozyme Publication year: 2012 Source: Journal of Magnetic Resonance, Available online 24 January 2012</br> Elka R.Georgieva, Aritro S.Roy, Vladimir M.Grigoryants, Petr P.Borbat, Keith A.*Earle, ...</br> Pulsed dipolar ESR spectroscopy, DEER and DQC, require frozen samples. An important issue in the biological application of this technique is how the freezing rate and concentration of cryoprotectant could possibly affect the...	nmrlearner	Journal club	0	01-25-2012 08:56 AM
Research Scientist Position in the Cryo-Electron Microscopy Facility at the New York Structural Biol - New York Structural Biology Center - New York, NY, United States Research Scientist Position in the Cryo-Electron Microscopy Facility at the New York Structural Biol - New York Structural Biology Center - New York, NY, United States The New York Structural Biology Center (NYSBC) seeks an experienced electron microscopist to join the staff of its Cryo-Electron Microscope Facility (http://cryoem.nysbc.org). The NYSBC is shared center that supports state-of-the-art research in cryo-EM, NMR, and X-ray. Cryo-EM facilities include four transmission electron microscopes and a new d... More...	nmrlearner	Job marketplace	0	01-26-2011 04:21 AM
[NMR paper] Gated electron transfers and electron pathways in azurin: a NMR dynamic study at mult Gated electron transfers and electron pathways in azurin: a NMR dynamic study at multiple fields and temperatures. Related Articles Gated electron transfers and electron pathways in azurin: a NMR dynamic study at multiple fields and temperatures. J Mol Biol. 2004 Oct 1;342(5):1599-611 Authors: Zhuravleva AV, Korzhnev DM, Kupce E, Arseniev AS, Billeter M, Orekhov VY Dynamic properties of electron transfer pathways in a small blue copper cupredoxin are explored using an extensive 15N NMR relaxation study of reduced Pseudomonas aeruginosa azurin...	nmrlearner	Journal club	0	11-24-2010 10:01 PM
[NMR paper] Structure distribution in an elastin-mimetic peptide (VPGVG)3 investigated by solid-s Structure distribution in an elastin-mimetic peptide (VPGVG)3 investigated by solid-state NMR. Related Articles Structure distribution in an elastin-mimetic peptide (VPGVG)3 investigated by solid-state NMR. J Am Chem Soc. 2004 Apr 7;126(13):4199-210 Authors: Yao XL, Hong M Elastin is an extracellular-matrix protein that imparts elasticity to tissues. We have used solid-state NMR to determine a number of distances and torsion angles in an elastin-mimetic peptide, (VPGVG)3, to understand the structural basis of elasticity. C-H and C-N distances...	nmrlearner	Journal club	0	11-24-2010 09:51 PM
[NMR paper] Structure, stability, and function of hDim1 investigated by NMR, circular dichroism, Structure, stability, and function of hDim1 investigated by NMR, circular dichroism, and mutational analysis. Related Articles Structure, stability, and function of hDim1 investigated by NMR, circular dichroism, and mutational analysis. Biochemistry. 2003 Aug 19;42(32):9609-18 Authors: Zhang YZ, Cheng H, Gould KL, Golemis EA, Roder H The 142 amino acid Dim1p protein is a component of the U4/U6.U5 tri-snRNP complex required for pre-mRNA splicing and interacts with multiple splicing-associated proteins. To gain further insight into the...	nmrlearner	Journal club	0	11-24-2010 09:16 PM
[NMR paper] Complexes of yeast adenylate kinase and nucleotides investigated by 1H NMR. Complexes of yeast adenylate kinase and nucleotides investigated by 1H NMR. Related Articles Complexes of yeast adenylate kinase and nucleotides investigated by 1H NMR. Biochemistry. 1991 Apr 30;30(17):4137-42 Authors: Vetter IR, Konrad M, RÃ¶sch P The role of one of the histidine residues present in many adenylate kinases (H36 in the porcine cytosolic enzyme) is highly disputed. We thus studied the yeast enzyme (AKye) containing this His residue. AKye is highly homologous to the Escherichia coli enzyme (AKec), a protein that is already well...	nmrlearner	Journal club	0	08-21-2010 11:16 PM
[NMR paper] Tertiary structure of two-electron reduced Megasphaera elsdenii flavodoxin and some i Tertiary structure of two-electron reduced Megasphaera elsdenii flavodoxin and some implications, as determined by two-dimensional 1H-NMR and restrained molecular dynamics. http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--www3.interscience.wiley.com-aboutus-images-wiley_interscience_pubmed_logo_FREE_120x27.gif Related Articles Tertiary structure of two-electron reduced Megasphaera elsdenii flavodoxin and some implications, as determined by two-dimensional 1H-NMR and restrained molecular dynamics. Eur J Biochem. 1990 Nov 26;194(1):185-98 Authors: ...	nmrlearner	Journal club	0	08-21-2010 11:04 PM

« Previous Thread | Next Thread »

Posting Rules
You may not post new threads You may not post replies You may not post attachments You may not edit your posts BB code is On Smilies are On [IMG] code is On HTML code is On Trackbacks are Off Pingbacks are Off Refbacks are Off