NMR paper Sample Preparation for Membrane Protein Structural Studies by Solid-State NMR.

		BioNMR > Educational resources > Journal club
[NMR paper] Sample Preparation for Membrane Protein Structural Studies by Solid-State NMR.

Webservers

NMR processing:

MDD

NMR assignment:

Backbone:

Side-chains:

NOEs:

Structure from NMR restraints:

Ab initio:

Fragment-based:

Rosetta-NMR (Robetta)

Template-based:

GeNMR

I-TASSER

Refinement:

Amber

Structure from chemical shifts:

Fragment-based:

Homology-based:

Torsion angles from chemical shifts:

Preditor

TALOS

Promega- Proline

Secondary structure from chemical shifts:

CSI (via RCI server)

TALOS

MICS caps, β-turns

d2D

PECAN

Flexibility from chemical shifts:

RCI

Interactions from chemical shifts:

HADDOCK

Chemical shifts re-referencing:

NMR model quality:

NOEs, other restraints:

Chemical shifts:

RDCs:

Pseudocontact shifts:

Protein geomtery:

SAVES2 or SAVES4

Quality Control Check

NMR spectrum prediction:

FANDAS

MestReS

V-NMR

Flexibility from structure:

Backbone S2

Methyl S2

B-factor

Molecular dynamics:

Gromacs

Amber

Antechamber

Chemical shifts prediction:

From structure:

Shiftx2

Sparta+

Camshift

CH3shift- Methyl

ArShift- Aromatic

ShiftS

Proshift

PPM

CheShift-2- Cα

From sequence:

Shifty

Camcoil

Poulsen_rc_CS

Disordered proteins:

MAXOCC

Format conversion & validation:

CCPN

From NMR-STAR 3.1

Validate NMR-STAR 3.1

NMR sample preparation:

Protein disorder:

DisMeta

Protein solubility:

Isotope labeling:

Solid-state NMR:

Thread Tools

Search this Thread

Rate Thread

Display Modes

07-30-2017, 08:04 PM

nmrlearner

Senior Member

Join Date: Jan 2005

Posts: 23,777

Points: 193,617, Level: 100

Level up: 0%, 0 Points needed

Activity: 50.7%

Last Achievements

Award-Showcase

NMR Credits: 0

NMR Points: 193,617

Downloads: 0

Uploads: 0

Sample Preparation for Membrane Protein Structural Studies by Solid-State NMR.

Sample Preparation for Membrane Protein Structural Studies by Solid-State NMR.

Related Articles Sample Preparation for Membrane Protein Structural Studies by Solid-State NMR.

Methods Mol Biol. 2017;1635:345-358

Authors: Lacabanne D, Kunert B, Gardiennet C, Meier BH, Bo Ckmann A

Abstract
Conformational studies of membrane proteins remain a challenge in the field of structural biology, and in particular the investigation of the proteins in a native-like lipid environment. Solid-state NMR presents a valuable opportunity for this, and we present here three critical steps in the solid-state NMR sample preparation, i.e., membrane reconstitution of the protein in native lipids, rotor filling, and sample quality assessment, at the example of the Bacillus subtilis ATP-binding cassette transporter BmrA.

PMID: 28755379 [PubMed - in process]

More...

Did you find this post helpful?

Similar Threads
Thread	Thread Starter	Forum	Replies	Last Post
Efficient DNP NMR of membrane proteins: sample preparation protocols, sensitivity, and radical location From The DNP-NMR Blog: Efficient DNP NMR of membrane proteins: sample preparation protocols, sensitivity, and radical location Liao, S.Y., et al., Efficient DNP NMR of membrane proteins: sample preparation protocols, sensitivity, and radical location. J Biomol NMR, 2016: p. 1-15. http://www.ncbi.nlm.nih.gov/pubmed/26873390	nmrlearner	News from NMR blogs	0	02-25-2016 05:21 AM
[NMR paper] Efficient DNP NMR of membrane proteins: sample preparation protocols, sensitivity, and radical location. Efficient DNP NMR of membrane proteins: sample preparation protocols, sensitivity, and radical location. Related Articles Efficient DNP NMR of membrane proteins: sample preparation protocols, sensitivity, and radical location. J Biomol NMR. 2016 Feb 12; Authors: Liao SY, Lee M, Wang T, Sergeyev IV, Hong M Abstract Although dynamic nuclear polarization (DNP) has dramatically enhanced solid-state NMR spectral sensitivities of many synthetic materials and some biological macromolecules, recent studies of membrane-protein DNP using...	nmrlearner	Journal club	0	02-14-2016 08:25 PM
Efficient DNP NMR of membrane proteins: sample preparation protocols, sensitivity, and radical location Efficient DNP NMR of membrane proteins: sample preparation protocols, sensitivity, and radical location Abstract Although dynamic nuclear polarization (DNP) has dramatically enhanced solid-state NMR spectral sensitivities of many synthetic materials and some biological macromolecules, recent studies of membrane-protein DNP using exogenously doped paramagnetic radicals as polarizing agents have reported varied and sometimes surprisingly limited enhancement factors. This motivated us to carry out a systematic evaluation of sample preparation protocols...	nmrlearner	Journal club	0	02-12-2016 11:26 PM
Optimizing sample preparation methods for dynamic nuclear polarization solid-state NMR of synthetic polymers From The DNP-NMR Blog: Optimizing sample preparation methods for dynamic nuclear polarization solid-state NMR of synthetic polymers Le, D., et al., Optimizing sample preparation methods for dynamic nuclear polarization solid-state NMR of synthetic polymers. Macromolecules, 2014: p. 140613123939001. http://pubs.acs.org/doi/abs/10.1021/ma500788n	nmrlearner	News from NMR blogs	0	06-18-2014 06:09 PM
[NMR paper] Membrane Protein Structural Validation by Oriented Sample Solid-State NMR: Diacylglycerol Kinase. Membrane Protein Structural Validation by Oriented Sample Solid-State NMR: Diacylglycerol Kinase. Related Articles Membrane Protein Structural Validation by Oriented Sample Solid-State NMR: Diacylglycerol Kinase. Biophys J. 2014 Apr 15;106(8):1559-69 Authors: Murray DT, Li C, Gao FP, Qin H, Cross TA Abstract The validation of protein structures through functional assays has been the norm for many years. Functional assays perform this validation for water-soluble proteins very well, but they need to be performed in the same...	nmrlearner	Journal club	0	04-18-2014 01:35 PM
Membrane Protein Structural Validation by Oriented Sample Solid-State NMR: Diacylglycerol Kinase Membrane Protein Structural Validation by Oriented Sample Solid-State NMR: Diacylglycerol Kinase Publication date: 15 April 2014 Source:Biophysical Journal, Volume 106, Issue 8</br> Author(s): DylanT. Murray , Conggang Li , F.Philip Gao , Huajun Qin , Timothy*A. Cross</br> The validation of protein structures through functional assays has been the norm for many years. Functional assays perform this validation for water-soluble proteins very well, but they need to be performed in the same environment as that used for the structural analysis. This is difficult...	nmrlearner	Journal club	0	04-15-2014 11:00 PM
[NMR paper] Resonance assignments of a membrane protein in phospholipid bilayers by combining multiple strategies of oriented sample solid-state NMR. Resonance assignments of a membrane protein in phospholipid bilayers by combining multiple strategies of oriented sample solid-state NMR. Related Articles Resonance assignments of a membrane protein in phospholipid bilayers by combining multiple strategies of oriented sample solid-state NMR. J Biomol NMR. 2013 Dec 20; Authors: Lu GJ, Opella SJ Abstract Oriented sample solid-state NMR spectroscopy can be used to determine the three-dimensional structures of membrane proteins in magnetically or mechanically aligned lipid bilayers. The...	nmrlearner	Journal club	0	12-21-2013 03:15 PM
Tertiary Structural Models for a Three Helix Membrane Protein ina Bilayer Environment from Oriented Sample Solid State NMR Data Tertiary Structural Models for a Three Helix Membrane Protein ina Bilayer Environment from Oriented Sample Solid State NMR Data 29 January 2013 Publication year: 2013 Source:Biophysical Journal, Volume 104, Issue 2, Supplement 1</br> </br> </br> </br></br>	nmrlearner	Journal club	0	02-03-2013 10:13 AM

« Previous Thread | Next Thread »

Posting Rules
You may not post new threads You may not post replies You may not post attachments You may not edit your posts BB code is On Smilies are On [IMG] code is On HTML code is On Trackbacks are Off Pingbacks are Off Refbacks are Off