[NMR paper] (113) Cd NMR Experiments Reveal an Unusual Metal Cluster in the Solution Structure of the Yeast Splicing Protein Bud31p.
(113) Cd NMR Experiments Reveal an Unusual Metal Cluster in the Solution Structure of the Yeast Splicing Protein Bud31p.
(113) Cd NMR Experiments Reveal an Unusual Metal Cluster in the Solution Structure of the Yeast Splicing Protein Bud31p.
Angew Chem Int Ed Engl. 2015 Feb 20;
Authors: van Roon AM, Yang JC, Mathieu D, Bermel W, Nagai K, Neuhaus D
Abstract
Establishing the binding topology of structural zinc ions in proteins is an essential part of their structure determination by NMR spectroscopy. Using (113) Cd NMR experiments...
[NMR paper] NMR investigation of the equilibrium partitioning of a water-soluble bile salt protein carrier to phospholipid vesicles.
NMR investigation of the equilibrium partitioning of a water-soluble bile salt protein carrier to phospholipid vesicles.
Related Articles NMR investigation of the equilibrium partitioning of a water-soluble bile salt protein carrier to phospholipid vesicles.
Proteins. 2013 Jun 12;
Authors: Ceccon A, D'Onofrio M, Zanzoni S, Longo DL, Aime S, Molinari H, Assfalg M
Abstract
Membrane binding by cytosolic fatty acid binding proteins (FABP) appears to constitute a key step of intracellular lipid trafficking. We applied NMR spectroscopy to study...
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Segmental isotopic labeling of a 140 kDa dimeric multi-domain protein CheA from Escherichia coli by expressed protein ligation and protein trans-splicing
Segmental isotopic labeling of a 140 kDa dimeric multi-domain protein CheA from Escherichia coli by expressed protein ligation and protein trans-splicing
Abstract Segmental isotopic labeling is a powerful labeling tool to facilitate NMR studies of larger proteins by not only alleviating the signal overlap problem but also retaining features of uniform isotopic labeling. Although two approaches, expressed protein ligation (EPL) and protein trans-splicing (PTS), have been mainly used for segmental isotopic labeling, there has been no single example in which both approaches have been...
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07-02-2012 06:18 AM
[NMR paper] Semisynthesis of a segmental isotopically labeled protein splicing precursor: NMR evi
Semisynthesis of a segmental isotopically labeled protein splicing precursor: NMR evidence for an unusual peptide bond at the N-extein-intein junction.
Related Articles Semisynthesis of a segmental isotopically labeled protein splicing precursor: NMR evidence for an unusual peptide bond at the N-extein-intein junction.
Proc Natl Acad Sci U S A. 2004 Apr 27;101(17):6397-402
Authors: Romanelli A, Shekhtman A, Cowburn D, Muir TW
Protein splicing is a posttranslational autocatalytic process in which an intervening sequence, termed an intein, is...
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[NMR paper] Salt-dependent structure change and ion binding in cytochrome c studied by two-dimens
Salt-dependent structure change and ion binding in cytochrome c studied by two-dimensional proton NMR.
Related Articles Salt-dependent structure change and ion binding in cytochrome c studied by two-dimensional proton NMR.
Biochemistry. 1990 Apr 10;29(14):3505-9
Authors: Feng Y, Englander SW
To search for salt-dependent structure changes that might help to explain physicochemical differences observed in previous solution studies, two-dimensional proton NMR spectra of reduced and oxidized cytochrome c were recorded at relatively high and low...
Salt-Dependent Conditional Protein Splicing of anIntein from Halobacterium salinarum
Linear Mode
