Related Articles?-(S)-Trifluoromethyl proline: evaluation as a structural substitute of proline for solid state (19)F-NMR peptide studies.
Org Biomol Chem. 2015 Mar 21;13(11):3171-81
Authors: Kubyshkin V, Afonin S, Kara S, Budisa N, Mykhailiuk PK, Ulrich AS
Abstract
?-(4S)-Trifluoromethyl proline was synthesised according to a modified literature protocol with improved yield on a multigram scale. Conformational properties of the amide bond formed by the amino acid were characterised using N-acetyl methyl ester model. The amide populations (s-trans vs. s-cis) and thermodynamic parameters of the isomerization were found to be similar to the corresponding values for intact proline. Therefore, the ?-trifluoromethyl proline was suggested as a structurally low-disturbing proline substitution in peptides for their structural studies by (19)F-NMR. Indeed, the exchange of native proline for ?-trifluoromethyl proline in the peptide antibiotic gramicidin S was shown to preserve the overall amphipathic peptide structure. The utility of the amino acid as a selective (19)F-NMR label was demonstrated by observing the re-alignment of the labelled gramicidin S in oriented lipid bilayers.
[NMR images] 1D 1H, pH 7.4 spectrum for L-Proline, bmse000047
http://bmrb.protein.osaka-u.ac.jp/metabolomics/standards/L_proline/nmr/bmse000047/spectra_png/1H/00.png
8/04/2014 7:46:05 AM GMT
1D 1H, pH 7.4 spectrum for L-Proline, bmse000047
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nmrlearner
NMR pictures
0
04-08-2014 07:45 AM
[NMR paper] Conformational change of Sos-derived proline-rich peptide upon binding Grb2 N-terminal SH3 domain probed by NMR.
Conformational change of Sos-derived proline-rich peptide upon binding Grb2 N-terminal SH3 domain probed by NMR.
http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--www.nature.com-images-lo_npg.gif Related Articles Conformational change of Sos-derived proline-rich peptide upon binding Grb2 N-terminal SH3 domain probed by NMR.
Sci Rep. 2013;3:2913
Authors: Ogura K, Okamura H
Abstract
Growth factor receptor-bound protein 2 (Grb2) is a small adapter protein composed of a single SH2 domain flanked by two SH3 domains. The...
nmrlearner
Journal club
0
10-10-2013 09:38 PM
[KPWU blog] Ramachandran space of Glycine and Proline
Ramachandran space of Glycine and Proline
The following two plots are made according to the statistical values provided by the Richardson group. I download the KINEMAGE format of Glycine and Proline. Inside the two files, core and allowed regions are defined and can be extracted to make my own Ramachandran plot. The defined core and allowed regions are also shown in http://stats.wordpress.com/b.gif?host=kpwu.wordpress.com&blog=76132&post=397&subd=kpwu&ref=&feed=1
Go to KPWU blog to read complete post.
nmrlearner
News from NMR blogs
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06-18-2011 03:04 AM
[NMR paper] Solid-state NMR investigation of the buried X-proline peptide bonds of bacteriorhodop
Solid-state NMR investigation of the buried X-proline peptide bonds of bacteriorhodopsin.
Related Articles Solid-state NMR investigation of the buried X-proline peptide bonds of bacteriorhodopsin.
Biochemistry. 2003 Apr 1;42(12):3586-93
Authors: Lansing JC, Hu JG, Belenky M, Griffin RG, Herzfeld J
The role of proline residues in the photocycle of bacteriorhodopsin (bR) is addressed using solid-state NMR. (13)C and (15)N chemical shifts from X-Pro peptide bonds in bR are assigned from REDOR difference spectra of pairwise labeled samples, and...
nmrlearner
Journal club
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11-24-2010 09:01 PM
[NMR paper] Birch pollen profilin: structural organization and interaction with poly-(L-proline)
Birch pollen profilin: structural organization and interaction with poly-(L-proline) peptides as revealed by NMR.
http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--linkinghub.elsevier.com-ihub-images-PubMedLink.gif Related Articles Birch pollen profilin: structural organization and interaction with poly-(L-proline) peptides as revealed by NMR.
FEBS Lett. 1997 Jul 14;411(2-3):291-5
Authors: Domke T, Federau T, Schlüter K, Giehl K, Valenta R, Schomburg D, Jockusch BM
The secondary structure of birch pollen profilin, a potent human...
nmrlearner
Journal club
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08-22-2010 05:08 PM
[NMR paper] Proline pipe helix: structure of the tus proline repeat determined by 1H NMR.
Proline pipe helix: structure of the tus proline repeat determined by 1H NMR.
http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--pubs.acs.org-images-acspubs.jpg Related Articles Proline pipe helix: structure of the tus proline repeat determined by 1H NMR.
Biochemistry. 1996 Jan 23;35(3):698-703
Authors: Butcher DJ, Nedved ML, Neiss TG, Moe GR
The structure of a 22 amino acid peptide, TPPI , that is similar to the proline repeat segment of the replication arrest protein, Tus, has been determined by 1H NMR in 50% trifluroethanol. The...
nmrlearner
Journal club
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08-22-2010 02:27 PM
[NMR paper] NMR structural studies of the tight complex between a trifluoromethyl ketone inhibito
NMR structural studies of the tight complex between a trifluoromethyl ketone inhibitor and the 85-kDa human phospholipase A2.
Related Articles NMR structural studies of the tight complex between a trifluoromethyl ketone inhibitor and the 85-kDa human phospholipase A2.
Biochemistry. 1993 Nov 30;32(47):12560-5
Authors: Trimble LA, Street IP, Perrier H, Tremblay NM, Weech PK, Bernstein MA
Arachidonyl trifluoromethyl ketone (AACOCF3) is a slow- and tight-binding inhibitor of the human cytosolic phospholipase A2 (cPLA2) . 19F and 13C NMR...
nmrlearner
Journal club
0
08-22-2010 03:01 AM
[NMR paper] The rate and structural consequences of proline cis-trans isomerization in calbindin
The rate and structural consequences of proline cis-trans isomerization in calbindin D9k: NMR studies of the minor (cis-Pro43) isoform and the Pro43Gly mutant.
Related Articles The rate and structural consequences of proline cis-trans isomerization in calbindin D9k: NMR studies of the minor (cis-Pro43) isoform and the Pro43Gly mutant.
Biochemistry. 1990 May 8;29(18):4400-9
Authors: Kördel J, Forsén S, Drakenberg T, Chazin WJ
The EF-hand calcium-binding protein, calbindin D9k, exists in solution in the calcium-loaded state, as a 1:3...