[NMR paper] Protein Rotational Dynamics in Aligned Lipid Membranes Probed by Anisotropic T1? NMR Relaxation.
Protein Rotational Dynamics in Aligned Lipid Membranes Probed by Anisotropic T1? NMR Relaxation.
Related Articles Protein Rotational Dynamics in Aligned Lipid Membranes Probed by Anisotropic T1? NMR Relaxation.
Biophys J. 2018 Jan 23;114(2):392-399
Authors: Awosanya EO, Nevzorov AA
Abstract
A membrane-bound form of Pf1 coat protein reconstituted in magnetically aligned DMPC/DHPC bicelles was used as a molecular probe to quantify for the viscosity of the lipid membrane interior by measuring the uniaxial rotational diffusion...
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02-06-2018 07:42 PM
Protein Rotational Dynamics in Aligned Lipid Membranes Probed by Anisotropic T1? NMR Relaxation
Protein Rotational Dynamics in Aligned Lipid Membranes Probed by Anisotropic T1? NMR Relaxation
Publication date: 23 January 2018
Source:Biophysical Journal, Volume 114, Issue 2</br>
Author(s): Emmanuel O. Awosanya, Alexander A. Nevzorov</br>
A membrane-bound form of Pf1 coat protein reconstituted in magnetically aligned DMPC/DHPC bicelles was used as a molecular probe to quantify for the viscosity of the lipid membrane interior by measuring the uniaxial rotational diffusion coefficient of the protein. Orientationally dependent 15N NMR relaxation times in the...
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02-03-2018 02:16 PM
DNP-enhanced NMR on aligned Lipid Bilayers at ambient Temperature
From The DNP-NMR Blog:
DNP-enhanced NMR on aligned Lipid Bilayers at ambient Temperature
Jakdetchai, O., et al., DNP-enhanced NMR on aligned Lipid Bilayers at ambient Temperature. J Am Chem Soc, 2014.
http://www.ncbi.nlm.nih.gov/pubmed/25333422
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10-24-2014 07:18 PM
[NMR paper] DNP-enhanced NMR on aligned Lipid Bilayers at ambient Temperature.
DNP-enhanced NMR on aligned Lipid Bilayers at ambient Temperature.
Related Articles DNP-enhanced NMR on aligned Lipid Bilayers at ambient Temperature.
J Am Chem Soc. 2014 Oct 21;
Authors: Jakdetchai O, Denysenkov V, Becker-Baldus J, Dutagaci B, Prisner T, Glaubitz C
Abstract
DNP-enhanced solid-state NMR has been shown to hold great potential for functional studies of membrane proteins at low temperatures due to its great sensitivity improvement. There are however numerous applications, for which experiments at ambient temperature...
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10-22-2014 12:34 PM
[NMR paper] Dipolar Assisted Assignment Protocol (DAAP) for MAS solid-state NMR of rotationally aligned membrane proteins in phospholipid bilayers.
Dipolar Assisted Assignment Protocol (DAAP) for MAS solid-state NMR of rotationally aligned membrane proteins in phospholipid bilayers.
Dipolar Assisted Assignment Protocol (DAAP) for MAS solid-state NMR of rotationally aligned membrane proteins in phospholipid bilayers.
J Magn Reson. 2014 Mar 1;242C:224-232
Authors: Das BB, Zhang H, Opella SJ
Abstract
A method for making resonance assignments in magic angle spinning solid-state NMR spectra of membrane proteins that utilizes the range of heteronuclear dipolar coupling...
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04-06-2014 02:01 AM
Dipolar Assisted Assignment Protocol (DAAP) for MAS solid-state NMR of Rotationally Aligned Membrane Proteins in Phospholipid Bilayers
Dipolar Assisted Assignment Protocol (DAAP) for MAS solid-state NMR of Rotationally Aligned Membrane Proteins in Phospholipid Bilayers
Publication date: Available online 1 March 2014
Source:Journal of Magnetic Resonance</br>
Author(s): Bibhuti B. Das , Hua Zhang , Stanley J. Opella</br>
A method for making resonance assignments in magic angle spinning solid-state NMR spectra of membrane proteins that utilizes the range of hetero-nuclear dipolar coupling frequencies in combination with conventional chemical shift based assignment methods is demonstrated. The...
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03-01-2014 11:11 AM
Multidimensional oriented solid-state NMR experiments enable the sequential assignment of uniformly 15N labeled integral membrane proteins in magnetically aligned lipid bilayers
Multidimensional oriented solid-state NMR experiments enable the sequential assignment of uniformly 15N labeled integral membrane proteins in magnetically aligned lipid bilayers
Abstract Oriented solid-state NMR is the most direct methodology to obtain the orientation of membrane proteins with respect to the lipid bilayer. The method consists of measuring 1H-15N dipolar couplings (DC) and 15N anisotropic chemical shifts (CSA) for membrane proteins that are uniformly aligned with respect to the membrane bilayer. A significant advantage of this approach is that tilt and azimuthal...
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10-10-2011 06:27 AM
[NMR paper] Anisotropic rotational diffusion of perdeuterated HIV protease from 15N NMR relaxatio
Anisotropic rotational diffusion of perdeuterated HIV protease from 15N NMR relaxation measurements at two magnetic fields.
Related Articles Anisotropic rotational diffusion of perdeuterated HIV protease from 15N NMR relaxation measurements at two magnetic fields.
J Biomol NMR. 1996 Oct;8(3):273-84
Authors: Tjandra N, Wingfield P, Stahl S, Bax A
15N NMR relaxation times in perdeuterated HIV-1 protease, complexed with the sub-nanomolar inhibitor DMP323, have been measured at 600 and 360 MHz 1H frequency. The relative magnitudes of the principal...
Rotational Diffusion of Membrane Proteins Probed by Anisotropic T2 and T1? NMR Relaxation in Aligned Lipid Bilayers
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