[NMR paper] Role of rotational motion in diffusion NMR experiments on supramolecular assemblies: application to Sup35NM fibrils
Role of rotational motion in diffusion NMR experiments on supramolecular assemblies: application to Sup35NM fibrils
Pulsed-field gradient (PFG) NMR is an important tool for characterization of biomolecules and supramolecular assemblies. However, for micron-sized objects, such as amyloid fibrils, these experiments become difficult to interpret because in addition to translational diffusion they are also sensitive to rotational diffusion. We have constructed a mathematical theory describing the outcome of PFG NMR experiments on rod-like fibrils. To test its validity, we have studied the...
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04-24-2021 04:45 PM
Nitroxide Derivatives for Dynamic Nuclear Polarization in Liquids: The Role of Rotational Diffusion #DNPNMR
From The DNP-NMR Blog:
Nitroxide Derivatives for Dynamic Nuclear Polarization in Liquids: The Role of Rotational Diffusion #DNPNMR
Levien, M., M. Hiller, I. Tkach, M. Bennati, and T. Orlando. “Nitroxide Derivatives for Dynamic Nuclear Polarization in Liquids: The Role of Rotational Diffusion.” The Journal of Physical Chemistry Letters 11, no. 5 (March 5, 2020): 1629–35.
https://doi.org/10.1021/acs.jpclett.0c00270.
Polarization transfer efficiency in liquid-state dynamic nuclear polarization (DNP) depends on the interaction between polarizing agents (PAs) and target nuclei...
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08-07-2020 08:10 PM
[NMR paper] Accurate Prediction of Protein NMR Spin Relaxation by Means of Polarizable Force Fields. Application to Strongly Anisotropic Rotational Diffusion.
Accurate Prediction of Protein NMR Spin Relaxation by Means of Polarizable Force Fields. Application to Strongly Anisotropic Rotational Diffusion.
Related Articles Accurate Prediction of Protein NMR Spin Relaxation by Means of Polarizable Force Fields. Application to Strongly Anisotropic Rotational Diffusion.
J Phys Chem B. 2020 Jun 05;:
Authors: Marcellini M, Nguyen MH, Martin M, Hologne M, Walker O
Abstract
Among the various biophysical methods available to investigate protein dynamics, NMR present the ability to scrutinize...
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06-06-2020 04:36 PM
[NMR paper] Solid-state NMR spectroscopic trends for supramolecular assemblies and protein aggregates.
Solid-state NMR spectroscopic trends for supramolecular assemblies and protein aggregates.
Related Articles Solid-state NMR spectroscopic trends for supramolecular assemblies and protein aggregates.
Solid State Nucl Magn Reson. 2017 Aug 24;87:45-53
Authors: Linser R
Abstract
Solid-state NMR is able to generate structural data on sample preparations that are explicitly non-crystalline. In particular, for amyloid fibril samples, which can comprise significant degrees of sample disorder, solid-state NMR has been used very...
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09-05-2017 12:41 PM
Solid-state NMR spectroscopic trends for supramolecular assemblies and protein aggregates
Solid-state NMR spectroscopic trends for supramolecular assemblies and protein aggregates
Publication date: Available online 24 August 2017
Source:Solid State Nuclear Magnetic Resonance</br>
Author(s): Rasmus Linser</br>
Solid-state NMR is able to generate structural data on sample preparations that are explicitly non-crystalline. In particular, for amyloid fibril samples, which can comprise significant degrees of sample disorder, solid-state NMR has been used very successfully. But also solid-state NMR studies of other supramolecular assemblies that have resisted...
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08-25-2017 04:11 AM
[NMR paper] Structural biology of supramolecular assemblies by magic-angle spinning NMR spectroscopy.
Structural biology of supramolecular assemblies by magic-angle spinning NMR spectroscopy.
Related Articles Structural biology of supramolecular assemblies by magic-angle spinning NMR spectroscopy.
Q Rev Biophys. 2017 Jan;50:e1
Authors: Quinn CM, Polenova T
Abstract
In recent years, exciting developments in instrument technology and experimental methodology have advanced the field of magic-angle spinning (MAS) nuclear magnetic resonance (NMR) to new heights. Contemporary MAS NMR yields atomic-level insights into structure and...
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01-19-2017 08:56 AM
[NMR paper] Harnessing NMR relaxation interference effects to characterise supramolecular assemblies.
Harnessing NMR relaxation interference effects to characterise supramolecular assemblies.
Related Articles Harnessing NMR relaxation interference effects to characterise supramolecular assemblies.
Chem Commun (Camb). 2016 Apr 28;
Authors: Karunanithy G, Cnossen A, Müller H, Peeks MD, Rees NH, Claridge TD, Anderson HL, Baldwin AJ
Abstract
Solution-state NMR spectroscopy remains the primary method for characterising synthetic supramolecular assemblies. Yet, in their NMR spectra, relaxation interference effects can significantly...
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04-29-2016 06:31 PM
A systematic mutagenesis-driven strategy for site-resolved NMR studies of supramolecular assemblies.
A systematic mutagenesis-driven strategy for site-resolved NMR studies of supramolecular assemblies.
A systematic mutagenesis-driven strategy for site-resolved NMR studies of supramolecular assemblies.
J Biomol NMR. 2011 May 29;
Authors: Amero C, Asunción Durá M, Noirclerc-Savoye M, Perollier A, Gallet B, Plevin MJ, Vernet T, Franzetti B, Boisbouvier J
Obtaining sequence-specific assignments remains a major bottleneck in solution NMR investigations of supramolecular structure, dynamics and interactions. Here we demonstrate that resonance...
Role of rotational motion in diffusion NMR experiments on supramolecular assemblies: application to Sup35NM fibrils
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