NMR paper The role of coherence transfer efficiency in design of TROSY-type multidimensional NM

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[NMR paper] The role of coherence transfer efficiency in design of TROSY-type multidimensional NM

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NMR assignment:

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Side-chains:

NOEs:

Structure from NMR restraints:

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Torsion angles from chemical shifts:

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Secondary structure from chemical shifts:

CSI (via RCI server)

TALOS

MICS caps, β-turns

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CheShift-2- Cα

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Shifty

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11-18-2010, 08:31 PM

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The role of coherence transfer efficiency in design of TROSY-type multidimensional NM

The role of coherence transfer efficiency in design of TROSY-type multidimensional NMR experiments.

Related Articles The role of coherence transfer efficiency in design of TROSY-type multidimensional NMR experiments.

J Magn Reson. 1999 Aug;139(2):439-42

Authors: Meissner A, Sørensen OW

An improved method for TROSY-type (Pervushin et al., Proc. Natl. Acad. Sci. USA 94, 12366-12371 (1997)) heteronuclear two-dimensional correlation involving protons of negligible CSA is presented. Rather than applying a simple INEPT sequence for back-transfer to protons (Pervushin et al., J. Am. Chem. Soc. 120, 6394-6400 (1998)), we replace the pi/2 proton pulse in INEPT by a spin-state-selective coherence transfer element (Sorensen et al., J. Biomol. NMR 10, 181-186 (1997)) and maintain broadband decoupling during acquisition. Theoretically that results in a sensitivity enhancement of a factor of 2. The new method is demonstrated using a (13)C,(15)N-labeled protein sample, RAP 18-112 (N-terminal domain of alpha(2)-macroglobulin receptor associated protein), at 750 MHz.

PMID: 10423383 [PubMed - indexed for MEDLINE]

Source: PubMed

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