Related ArticlesRNA structure determination by solid-state NMR spectroscopy.
Nat Commun. 2015;6:7024
Authors: Marchanka A, Simon B, Althoff-Ospelt G, Carlomagno T
Abstract
Knowledge of the RNA three-dimensional structure, either in isolation or as part of RNP complexes, is fundamental to understand the mechanism of numerous cellular processes. Because of its flexibility, RNA represents a challenge for crystallization, while the large size of cellular complexes brings solution-state NMR to its limits. Here, we demonstrate an alternative approach on the basis of solid-state NMR spectroscopy. We develop a suite of experiments and RNA labeling schemes and demonstrate for the first time that ssNMR can yield a RNA structure at high-resolution. This methodology allows structural analysis of segmentally labelled RNA stretches in high-molecular weight cellular machines-independent of their ability to crystallize- and opens the way to mechanistic studies of currently difficult-to-access RNA-protein assemblies.
[NMR paper] De Novo 3D Structure Determination from Sub-milligram Protein Samples by Solid-State 100 kHz MAS NMR Spectroscopy.
De Novo 3D Structure Determination from Sub-milligram Protein Samples by Solid-State 100 kHz MAS NMR Spectroscopy.
De Novo 3D Structure Determination from Sub-milligram Protein Samples by Solid-State 100 kHz MAS NMR Spectroscopy.
Angew Chem Int Ed Engl. 2014 Sep 15;
Authors: Agarwal V, Penzel S, Szekely K, Cadalbert R, Testori E, Oss A, Past J, Samoson A, Ernst M, Böckmann A, Meier BH
Abstract
Solid-state NMR spectroscopy is an emerging tool for structural studies of crystalline, membrane-associated, sedimented, and fibrillar...
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09-17-2014 11:54 AM
Towards structure determination of self-assembled peptides using dynamic nuclear polarization enhanced solid-state NMR spectroscopy
From The DNP-NMR Blog:
Towards structure determination of self-assembled peptides using dynamic nuclear polarization enhanced solid-state NMR spectroscopy
Takahashi, H., et al., Towards structure determination of self-assembled peptides using dynamic nuclear polarization enhanced solid-state NMR spectroscopy. Angew Chem Int Ed Engl, 2013. 52(27): p. 6979-82.
http://www.ncbi.nlm.nih.gov/pubmed/23564735
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04-23-2014 06:31 PM
[NMR paper] Solid-state NMR spectroscopy structure determination of a lipid-embedded heptahelical membrane protein.
Solid-state NMR spectroscopy structure determination of a lipid-embedded heptahelical membrane protein.
Solid-state NMR spectroscopy structure determination of a lipid-embedded heptahelical membrane protein.
Nat Methods. 2013 Sep 8;
Authors: Wang S, Munro RA, Shi L, Kawamura I, Okitsu T, Wada A, Kim SY, Jung KH, Brown LS, Ladizhansky V
Abstract
Determination of structure of integral membrane proteins, especially in their native environment, is a formidable challenge in structural biology. Here we demonstrate that magic angle spinning...
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09-10-2013 08:44 PM
Towards Structure Determination of Self-Assembled Peptides Using Dynamic Nuclear Polarization Enhanced Solid-State NMR Spectroscopy
From The DNP-NMR Blog:
Towards Structure Determination of Self-Assembled Peptides Using Dynamic Nuclear Polarization Enhanced Solid-State NMR Spectroscopy
Takahashi, H., et al., Towards Structure Determination of Self-Assembled Peptides Using Dynamic Nuclear Polarization Enhanced Solid-State NMR Spectroscopy. Angew. Chem. Int. Ed., 2013: p. n/a-n/a.
http://www.ncbi.nlm.nih.gov/pubmed/23564735
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06-13-2013 04:24 AM
[NMR paper] Protein Structure Determination with Paramagnetic Solid-State NMR Spectroscopy.
Protein Structure Determination with Paramagnetic Solid-State NMR Spectroscopy.
Related Articles Protein Structure Determination with Paramagnetic Solid-State NMR Spectroscopy.
Acc Chem Res. 2013 Mar 6;
Authors: Sengupta I, Nadaud PS, Jaroniec CP
Abstract
Many structures of the proteins and protein assemblies that play central roles in fundamental biological processes and disease pathogenesis are not readily accessible via the conventional techniques of single-crystal X-ray diffraction and solution-state nuclear magnetic resonance (NMR)....
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03-08-2013 10:35 PM
[NMR paper] Determination of membrane protein structure and dynamics by magic-angle-spinning solid-state NMR spectroscopy.
Determination of membrane protein structure and dynamics by magic-angle-spinning solid-state NMR spectroscopy.
Related Articles Determination of membrane protein structure and dynamics by magic-angle-spinning solid-state NMR spectroscopy.
J Am Chem Soc. 2005 Sep 21;127(37):12965-74
Authors: Andronesi OC, Becker S, Seidel K, Heise H, Young HS, Baldus M
It is shown that molecular structure and dynamics of a uniformly labeled membrane protein can be studied under magic-angle-spinning conditions. For this purpose, dipolar recoupling experiments...
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12-01-2010 06:56 PM
[NMR paper] Protein structure determination by high-resolution solid-state NMR spectroscopy: appl
Protein structure determination by high-resolution solid-state NMR spectroscopy: application to microcrystalline ubiquitin.
Related Articles Protein structure determination by high-resolution solid-state NMR spectroscopy: application to microcrystalline ubiquitin.
J Am Chem Soc. 2005 Jun 22;127(24):8618-26
Authors: Zech SG, Wand AJ, McDermott AE
High-resolution solid-state NMR spectroscopy has become a promising method for the determination of three-dimensional protein structures for systems which are difficult to crystallize or exhibit low...
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11-25-2010 08:21 PM
[NMR paper] A concept for rapid protein-structure determination by solid-state NMR spectroscopy.
A concept for rapid protein-structure determination by solid-state NMR spectroscopy.
Related Articles A concept for rapid protein-structure determination by solid-state NMR spectroscopy.
Angew Chem Int Ed Engl. 2005 Mar 29;44(14):2089-92
Authors: Lange A, Becker S, Seidel K, Giller K, Pongs O, Baldus M
RNA structure determination by solid-state NMR spectroscopy.
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