[NMR paper] Rigid, highly reactive and stable DOTA-like tags containing a thiol-specific phenylsulfonyl pyridine moiety for protein modification and NMR analysis
Site specific labeling of paramagnetic NMR tags generate valuable structural restraints such as pseudocontact shifts (PCSs) and residual dipolar couplings (RDCs) that can be used to characterize structures, interactions and dynamics of protein-ligand or nucleic acid complexes. Herein, two single-armed DOTA-like tags, BrPSPy-DO3M( S )A-Ln and BrPSPy-6M-DO3M( S )A-Ln, each containing a thiol-specific reacting group, phenylsulfonyl pyridine moiety, are demonstrated as rigid, reactive and stable...
[NMR paper] Stable and rigid DTPA-like paramagnetic tags suitable for in vitro and in situ protein NMR analysis.
Stable and rigid DTPA-like paramagnetic tags suitable for in vitro and in situ protein NMR analysis.
Stable and rigid DTPA-like paramagnetic tags suitable for in vitro and in situ protein NMR analysis.
J Biomol NMR. 2017 Dec 09;:
Authors: Chen JL, Zhao Y, Gong YJ, Pan BB, Wang X, Su XC
Abstract
Organic synthesis of a ligand with high binding affinities for paramagnetic lanthanide ions is an effective way of generating paramagnetic effects on proteins. These paramagnetic effects manifested in high-resolution NMR spectroscopy are...
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12-11-2017 12:45 PM
Stable and rigid DTPA-like paramagnetic tags suitable for in vitro and in situ protein NMR analysis
Stable and rigid DTPA-like paramagnetic tags suitable for in vitro and in situ protein NMR analysis
Abstract
Organic synthesis of a ligand with high binding affinities for paramagnetic lanthanide ions is an effective way of generating paramagnetic effects on proteins. These paramagnetic effects manifested in high-resolution NMR spectroscopy are valuable dynamic and structural restraints of proteins and proteinâ??ligand complexes. A paramagnetic tag generally contains a metal chelating moiety and a reactive group for protein modification. Herein we...
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12-10-2017 01:21 AM
[NMR paper] Site-specific tagging proteins with a rigid, small and stable transition metal chelator, 8-hydroxyquinoline, for paramagnetic NMR analysis.
Site-specific tagging proteins with a rigid, small and stable transition metal chelator, 8-hydroxyquinoline, for paramagnetic NMR analysis.
Related Articles Site-specific tagging proteins with a rigid, small and stable transition metal chelator, 8-hydroxyquinoline, for paramagnetic NMR analysis.
J Biomol NMR. 2016 Jan 6;
Authors: Yang Y, Huang F, Huber T, Su XC
Abstract
Design of a paramagnetic metal binding motif in a protein is a valuable way for understanding the function, dynamics and interactions of a protein by paramagnetic...
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01-07-2016 11:10 PM
Site-specific tagging proteins with a rigid, small and stable transition metal chelator, 8-hydroxyquinoline, for paramagnetic NMR analysis
Site-specific tagging proteins with a rigid, small and stable transition metal chelator, 8-hydroxyquinoline, for paramagnetic NMR analysis
Abstract
Design of a paramagnetic metal binding motif in a protein is a valuable way for understanding the function, dynamics and interactions of a protein by paramagnetic NMR spectroscopy. Several strategies have been proposed to site-specifically tag proteins with paramagnetic lanthanide ions. Here we report a simple approach of engineering a transition metal binding motif via site-specific labelling of a...
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01-06-2016 09:48 AM
[NMR paper] NMR solution structure of a highly stable de novo heterodimeric coiled-coil.
NMR solution structure of a highly stable de novo heterodimeric coiled-coil.
Related Articles NMR solution structure of a highly stable de novo heterodimeric coiled-coil.
Biopolymers. 2004 Dec 5;75(5):367-75
Authors: Lindhout DA, Litowski JR, Mercier P, Hodges RS, Sykes BD
The NMR solution structure of a highly stable coiled-coil IAAL-E3/K3 has been solved. The E3/K3 coiled-coil is a 42-residue de novo designed coiled-coil comprising three heptad repeats per subunit, stabilized by hydrophobic contacts within the core and electrostatic...
Rigid, highly reactive and stable DOTA-like tags containing a thiol-specific phenylsulfonyl pyridine moiety for protein modification and NMR analysis
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