The classical approach restricts the detection of metabolites in serum samples by using nuclear magnetic resonance (NMR) spectroscopy; however, the presence of copious proteins and lipoproteins emphasize and necessitate the development of a contemporary, high-throughput tactic. To eliminate the lipoproteins and proteins from sera to engender filtered sera (FS), the study was executed with 50 ?l serum obtained from five healthy individuals with 5 years of age difference from 25 to 45 years old...
[NMR paper] 1H NMR Reveals the Mechanism of Potassium Lactate on Proteolysis and Taste Metabolites of Rugao Ham
1H NMR Reveals the Mechanism of Potassium Lactate on Proteolysis and Taste Metabolites of Rugao Ham
To deepen the understanding of the effect of potassium lactate on the taste of Rugao ham, proteolysis index, enzyme activities and protein degradation of Rugao ham salted with potassium lactate (0%, 0.5%, 1%, 2%) were investigated. Metabolites of Rugao ham were identified by ¹H nuclear magnetic resonance (NMR) spectroscopy and the metabolic pathways of the key metabolites were enriched by the Kyoto Encyclopedia of Genes and Genomes (KEGG); the relationship between taste and metabolites...
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04-13-2023 10:58 AM
[NMR paper] A Comprehensive NMR Analysis of Serum and Fecal Metabolites in Familial Dysautonomia Patients Reveals Significant Metabolic Perturbations
A Comprehensive NMR Analysis of Serum and Fecal Metabolites in Familial Dysautonomia Patients Reveals Significant Metabolic Perturbations
Central metabolism has a profound impact on the clinical phenotypes and penetrance of neurological diseases such as Alzheimer's (AD) and Parkinson's (PD) diseases, Amyotrophic Lateral Sclerosis (ALS) and Autism Spectrum Disorder (ASD). In contrast to the multifactorial origin of these neurological diseases, neurodevelopmental impairment and neurodegeneration in Familial Dysautonomia (FD) results from a single point mutation in the ELP1 gene. FD patients...
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03-31-2023 09:21 AM
[NMR paper] Beyond the limit of assignment of metabolites using minimal serum samples and 1H NMR spectroscopy with cross-validation by mass spectrometry.
Beyond the limit of assignment of metabolites using minimal serum samples and 1H NMR spectroscopy with cross-validation by mass spectrometry.
Beyond the limit of assignment of metabolites using minimal serum samples and 1H NMR spectroscopy with cross-validation by mass spectrometry.
J Pharm Biomed Anal. 2018 Jan 09;151:356-364
Authors: Gupta A, Kumar D
Abstract
Identification of NMR-based metabolic indexes is limited by the deleterious effects of copious proteins and lipoproteins in the serum that accentuate the need for advance...
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02-08-2018 04:32 PM
Journal Highlight: Fertility-associated metabolites in bull seminal plasma and blood serum: 1H NMR analysis
Journal Highlight: Fertility-associated metabolites in bull seminal plasma and blood serum: 1H NMR analysis
http://www.spectroscopynow.com/common/images/thumbnails/14bd568ab8a.jpgA proof-of-principle study has been carried out to identify fertility-associated metabolites in dairy bull seminal plasma and blood serum using proton NMR spectroscopy.
Read the rest at Spectroscopynow.com
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03-02-2015 04:03 PM
[NMR paper] Quantitating Metabolites in Protein Precipitated Serum Using NMR Spectroscopy.
Quantitating Metabolites in Protein Precipitated Serum Using NMR Spectroscopy.
Related Articles Quantitating Metabolites in Protein Precipitated Serum Using NMR Spectroscopy.
Anal Chem. 2014 May 5;
Authors: Gowda GA, Raftery D
Abstract
Quantitative NMR-based metabolite profiling is challenged by the deleterious effects of abundant proteins in the intact blood plasma/serum, which underscores the need for alternative approaches. Protein removal by ultrafiltration using low molecular weight cut-off filters thus represents an...
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05-08-2014 05:26 AM
[NMR paper] Systemic and characteristic metabolites in the serum of streptozotocin-induced diabetic rats at different stages as revealed by a (1)H-NMR based metabonomic approach.
Systemic and characteristic metabolites in the serum of streptozotocin-induced diabetic rats at different stages as revealed by a (1)H-NMR based metabonomic approach.
Systemic and characteristic metabolites in the serum of streptozotocin-induced diabetic rats at different stages as revealed by a (1)H-NMR based metabonomic approach.
Mol Biosyst. 2014 Jan 21;
Authors: Diao C, Zhao L, Guan M, Zheng Y, Chen M, Yang Y, Lin L, Chen W, Gao H
Abstract
Diabetes mellitus is a typical heterogeneous metabolic disorder characterized by abnormal...
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01-23-2014 03:58 PM
[NMR paper] NMR metabolomics profiling of blood plasma mimics shows that medium- and long-chain fatty acids differently release metabolites from human serum albumin.
NMR metabolomics profiling of blood plasma mimics shows that medium- and long-chain fatty acids differently release metabolites from human serum albumin.
Related Articles NMR metabolomics profiling of blood plasma mimics shows that medium- and long-chain fatty acids differently release metabolites from human serum albumin.
J Magn Reson. 2013 Dec 12;239C:34-43
Authors: Jupin M, Michiels PJ, Girard FC, Spraul M, Wijmenga SS
Abstract
Metabolite profiling by NMR of body fluids is increasingly used to successfully differentiate patients...
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01-01-2014 03:05 PM
[NMR paper] NMR Metabolomics Profiling of Blood Plasma Mimics shows that Medium- and Long-chain Fatty Acids Differently Release Metabolites from Human Serum Albumin
NMR Metabolomics Profiling of Blood Plasma Mimics shows that Medium- and Long-chain Fatty Acids Differently Release Metabolites from Human Serum Albumin
Publication date: Available online 12 December 2013
Source:Journal of Magnetic Resonance</br>
Author(s): M.D. Jupin , P.J. Michiels , F.C. Girard , M. Spraul , S.S. Wijmenga</br>
Metabolite profiling by NMR of body fluids is increasingly used to successfully differentiate patients from healthy individuals. Metabolites and their concentrations are direct reporters of body biochemistry. However, in blood plasma...
Richer than previously probed: An application of 1H NMR reveals one hundred metabolites using only fifty microliter serum
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