Related ArticlesResolution of individual lipids in mixed phospholipid membranes and specific lipid-cytochrome c interactions by magic-angle spinning solid-state phosphorus-31 NMR.
Biochemistry. 1994 Mar 8;33(9):2459-67
Authors: Pinheiro TJ, Watts A
A model of the inner mitochondrial membrane was constructed with dioleoyphosphatidylcholine (PC), dioleoylphosphatidylethanolamine (PE), and cardiolipin (CL) at a PC:PE:CL molar ratio of 2:2:1, and the interaction of the peripheral membrane protein cytochrome c with this mixed membrane has been investigated by static and magic-angle spinning (MAS) solid-state 31P NMR. The static 31P NMR spectrum of the three-component membrane is a typical broad powder pattern for phospholipids in a bilayer structure, and is a result of three overlapping spectra of each individual phospholipid component in the mixed membrane, with an average effective chemical shift anisotropy of approximately 41 ppm. Using magic-angle spinning NMR methods, three resolved resonances are observed in the narrowed MAS 31P NMR spectrum, each of which has been assigned to each lipid component in the mixed membrane. This allows the investigation of individual phospholipid-protein interactions in multicomponent lipid bilayers. The interaction of cytochrome c with each lipid in a model mitochondrial membrane could now be evaluated. Phosphorus-31 spin-lattice (T1) relaxation times for each lipid phosphate were measured as a function of temperature, in the absence and presence of bound cytochrome c. T1 was not affected for any lipid upon binding of cytochrome c over the temperature range analyzed. However, averaging of the phosphorus-31 chemical shift anisotropy for the cardiolipin component in mixed PC/PE/CL bilayers at lower temperatures ceases to be axially symmetric on binding of cytochrome c, while for PC and PE components the axial symmetry is retained over the temperature interval studied here.(ABSTRACT TRUNCATED AT 250 WORDS)
[NMR paper] Interaction of the fusogenic peptide B18 in its amyloid-state with lipid membranes st
Interaction of the fusogenic peptide B18 in its amyloid-state with lipid membranes studied by solid state NMR.
Related Articles Interaction of the fusogenic peptide B18 in its amyloid-state with lipid membranes studied by solid state NMR.
Chem Phys Lipids. 2004 Nov;132(1):65-77
Authors: Grage SL, Afonin S, Grüne M, Ulrich AS
The interaction of the fusogenic polypeptide segment "B18" from the fertilization protein binding with lipid membranes was investigated by solid state 2H and 31P NMR, and by differential scanning calorimetry. B18 is known...
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[NMR paper] High-resolution 31p field cycling NMR as a probe of phospholipid dynamics.
High-resolution 31p field cycling NMR as a probe of phospholipid dynamics.
Related Articles High-resolution 31p field cycling NMR as a probe of phospholipid dynamics.
J Am Chem Soc. 2004 Oct 27;126(42):13765-77
Authors: Roberts MF, Redfield AG
We have used high-resolution field-cycling 31P NMR spectroscopy to measure spin-lattice relaxation rates (R1 = 1/T1) of multicomponent phospholipid vesicle and micelle samples over a large field range, from 0.1 to 11.7 T. The shape of the curve for R1 as a function of field and a model-free analysis were...
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[NMR paper] Membrane protein-lipid interactions in mixed micelles studied by NMR spectroscopy wit
Membrane protein-lipid interactions in mixed micelles studied by NMR spectroscopy with the use of paramagnetic reagents.
Related Articles Membrane protein-lipid interactions in mixed micelles studied by NMR spectroscopy with the use of paramagnetic reagents.
Chembiochem. 2004 Apr 2;5(4):467-73
Authors: Hilty C, Wider G, Fernández C, Wüthrich K
For solution NMR studies of the structure and function of membrane proteins, these macromolecules have to be reconstituted and solubilized in detergent micelles. Detailed characterization of the mixed...
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[NMR paper] Solid-state NMR studies of magnetically aligned phospholipid membranes: taming lantha
Solid-state NMR studies of magnetically aligned phospholipid membranes: taming lanthanides for membrane protein studies.
Related Articles Solid-state NMR studies of magnetically aligned phospholipid membranes: taming lanthanides for membrane protein studies.
Biochem Cell Biol. 1998;76(2-3):443-51
Authors: Prosser RS, Volkov VB, Shiyanovskaya IV
The addition of lanthanides (Tm3+, Yb3+, Er3+, or Eu3+) to a solution of long-chain phospholipids such as dimyristoylphosphatidylcholine (DMPC) and short-chain phospholipids such as...
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Lipid-Protein Correlations in Nanoscale Phospholipid Bilayers by Solid-State NMR.
Lipid-Protein Correlations in Nanoscale Phospholipid Bilayers by Solid-State NMR.
Lipid-Protein Correlations in Nanoscale Phospholipid Bilayers by Solid-State NMR.
Biochemistry. 2010 Aug 30;
Authors: Kijac A, Shih AY, Nieuwkoop AJ, Schulten K, Sligar SG, Rienstra CM
Nanodiscs are an example of discoidal nanoscale lipid/protein particles that have been extremely useful for the biochemical and biophysical characterization of membrane proteins. They are discoidal lipid bilayer fragments encircled and stabilized by two amphipathic helical...
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[NMR paper] Resolution of individual lipids in mixed phospholipid membranes and specific lipid-cy
Resolution of individual lipids in mixed phospholipid membranes and specific lipid-cytochrome c interactions by magic-angle spinning solid-state phosphorus-31 NMR.
Related Articles Resolution of individual lipids in mixed phospholipid membranes and specific lipid-cytochrome c interactions by magic-angle spinning solid-state phosphorus-31 NMR.
Biochemistry. 1994 Mar 8;33(9):2459-67
Authors: Pinheiro TJ, Watts A
A model of the inner mitochondrial membrane was constructed with dioleoyphosphatidylcholine (PC), dioleoylphosphatidylethanolamine...
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08-22-2010 03:33 AM
[NMR paper] Lipid specificity in the interaction of cytochrome c with anionic phospholipid bilaye
Lipid specificity in the interaction of cytochrome c with anionic phospholipid bilayers revealed by solid-state 31P NMR.
Related Articles Lipid specificity in the interaction of cytochrome c with anionic phospholipid bilayers revealed by solid-state 31P NMR.
Biochemistry. 1994 Mar 8;33(9):2451-8
Authors: Pinheiro TJ, Watts A
Phosphorus-31 NMR has been used to investigate the interaction of cytochrome c with bilayers of the anionic lipids dioleoylphosphatidylglycerol (DOPG), dioleoylphosphatidylserine (DOPS), and diacylphosphatidylinositol...
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[NMR paper] Lipid specificity in the interaction of cytochrome c with anionic phospholipid bilaye
Lipid specificity in the interaction of cytochrome c with anionic phospholipid bilayers revealed by solid-state 31P NMR.
Related Articles Lipid specificity in the interaction of cytochrome c with anionic phospholipid bilayers revealed by solid-state 31P NMR.
Biochemistry. 1994 Mar 8;33(9):2451-8
Authors: Pinheiro TJ, Watts A
Phosphorus-31 NMR has been used to investigate the interaction of cytochrome c with bilayers of the anionic lipids dioleoylphosphatidylglycerol (DOPG), dioleoylphosphatidylserine (DOPS), and diacylphosphatidylinositol...
Resolution of individual lipids in mixed phospholipid membranes and specific lipid-cy
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