2D NOESY and TOCSY play central roles in contemporary NMR. We have recently discussed how solvent-driven exchanges can significantly enhance the sensitivity of such methods when attempting correlations between labile and nonlabile protons. This study explores two scenarios where similar sensitivity enhancements can be achieved in the absence of solvent exchange: the first one involves biomolecular paramagnetic systems, while the other involves small organic molecules in natural abundance. It is...
[NMR paper] Non-invasive monitoring of in vitro gastric milk protein digestion kinetics by (1)H NMR magnetization transfer
Non-invasive monitoring of in vitro gastric milk protein digestion kinetics by (1)H NMR magnetization transfer
Processing of milk involves heating, which can modify the structure and digestibility of its proteins. In vitro models are useful for studying protein digestion. However, validating these models with in vivo data is challenging. Here, we non-invasively monitor in vitro gastric milk protein digestion by protein-water chemical exchange detected by ąH nuclear magnetic resonance (NMR) magnetization transfer (MT). We obtained either a fitted composite exchange rate (CER) with a...
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03-08-2022 04:48 PM
[NMR paper] Dry-cured ham tissue characterization by fast field cycling NMR relaxometry and quantitative magnetization transfer.
Dry-cured ham tissue characterization by fast field cycling NMR relaxometry and quantitative magnetization transfer.
Dry-cured ham tissue characterization by fast field cycling NMR relaxometry and quantitative magnetization transfer.
Magn Reson Chem. 2016 May 31;
Authors: Bajd F, Gradišek A, Apih T, Serša I
Abstract
Fast field cycling (FFC) and quantitative magnetization transfer (qMT) NMR methods are two powerful tools in NMR analysis of biological tissues. The qMT method is well established in biomedical NMR applications,...
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06-01-2016 11:08 AM
Recovery of bulk proton magnetization and sensitivity enhancement in ultrafast magic-angle spinning solid-state NMR
From The DNP-NMR Blog:
Recovery of bulk proton magnetization and sensitivity enhancement in ultrafast magic-angle spinning solid-state NMR
A large portion of the magnetization in a CP experiment remains unused after an experiment and different strategies exist to make better use of the proton magnetization. Here the authors show their results of testing 7 different cp schemes. Although not directly related to DNP these techniques are still very valuable to increase the sensitivity of an NMR experiment especially in combination with DNP.
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03-30-2015 06:04 PM
[NMR paper] Perspectives for sensitivity enhancement in proton-detected solid-state NMR of highly deuterated proteins by preserving water magnetization.
Perspectives for sensitivity enhancement in proton-detected solid-state NMR of highly deuterated proteins by preserving water magnetization.
Related Articles Perspectives for sensitivity enhancement in proton-detected solid-state NMR of highly deuterated proteins by preserving water magnetization.
J Biomol NMR. 2015 Jan 30;
Authors: Chevelkov V, Xiang S, Giller K, Becker S, Lange A, Reif B
Abstract
In this work, we show how the water flip-back approach that is widely employed in solution-state NMR can be adapted to...
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01-31-2015 04:16 PM
Perspectives for sensitivity enhancement in proton-detected solid-state NMR of highly deuterated proteins by preserving water magnetization
Perspectives for sensitivity enhancement in proton-detected solid-state NMR of highly deuterated proteins by preserving water magnetization
Abstract
In this work, we show how the water flip-back approach that is widely employed in solution-state NMR can be adapted to proton-detected MAS solid-state NMR of highly deuterated proteins. The scheme allows to enhance the sensitivity of the experiment by decreasing the recovery time of the proton longitudinal magnetization. The method relies on polarization transfer from non-saturated water to the protein...
[NMR paper] Dissection of heteronuclear NMR experiments for studies of magnetization transfer eff
Dissection of heteronuclear NMR experiments for studies of magnetization transfer efficiencies.
Related Articles Dissection of heteronuclear NMR experiments for studies of magnetization transfer efficiencies.
J Magn Reson. 2003 Nov;165(1):89-94
Authors: Braun D, Wüthrich K, Wider G
Modern NMR experiments for applications with biological macromolecules in solution typically include multiple magnetization transfer steps. When working with large structures, a significant fraction of the magnetization is lost during these transfers. For the design...
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11-24-2010 09:16 PM
[NMR paper] 19F NMR magnetization transfer between 5-FBAPTA and its complexes. An alternative mea
19F NMR magnetization transfer between 5-FBAPTA and its complexes. An alternative means for measuring free Ca2+ concentration, and detection of complexes with protein in erythrocytes.
Related Articles 19F NMR magnetization transfer between 5-FBAPTA and its complexes. An alternative means for measuring free Ca2+ concentration, and detection of complexes with protein in erythrocytes.
NMR Biomed. 1994 Nov;7(7):330-8
Authors: Gilboa H, Chapman BE, Kuchel PW
The 19F NMR Ca(2+)-indicator molecule 5,5'-difluoro-1,2-bis(o-...
Relaxation-Assisted Magnetization Transfer Phenomena for a Sensitivity-Enhanced 2D NMR
Linear Mode
