Proton exchange is a fundamental chemical event, and NMR provides the most direct readout of protonation events with site-specific resolution. Conventional approaches require manual titration of sample pH to collect a series of NMR spectra at different pH values. This requires extensive sample handling and often results in significant sample loss, leading to reduced signal or the need to prepare additional samples. Here, we introduce a novel approach to control pH in NMR samples using water...
[NMR paper] In situ light-driven pH modulation for NMR studies
In situ light-driven pH modulation for NMR studies
Proton exchange is a fundamental chemical event, and NMR provides the most direct readout of protonation events with site-specific resolution. Conventional approaches require manual titration of sample pH to collect a series of NMR spectra at different pH values. This requires extensive sample handling and often results in significant sample loss, leading to reduced signal or the need to prepare additional samples. Here, we introduce a novel approach to control pH in NMR samples using water...
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PrimaryTransfer Step in the Light-Driven Ion PumpBacteriorhodopsin: An Irreversible U-Turn Revealed by DynamicNuclear Polarization-Enhanced Magic Angle Spinning NMR
PrimaryTransfer Step in the Light-Driven Ion PumpBacteriorhodopsin: An Irreversible U-Turn Revealed by DynamicNuclear Polarization-Enhanced Magic Angle Spinning NMR
Qing Zhe Ni, Thach V. Can, Eugenio Daviso, Marina Belenky, Robert G. Griffin and Judith Herzfeld
https://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jacsat/0/jacsat.ahead-of-print/jacs.8b00022/20180312/images/medium/ja-2018-00022t_0006.gif
Journal of the American Chemical Society
DOI: 10.1021/jacs.8b00022
http://feeds.feedburner.com/~ff/acs/jacsat?d=yIl2AUoC8zA...
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03-13-2018 04:24 AM
500-fold enhancement of in situ (13)C liquid state NMR using gyrotron-driven temperature-jump DNP #DNPNMR
From The DNP-NMR Blog:
500-fold enhancement of in situ (13)C liquid state NMR using gyrotron-driven temperature-jump DNP #DNPNMR
Yoon, D., et al., 500-fold enhancement of in situ (13)C liquid state NMR using gyrotron-driven temperature-jump DNP. J Magn Reson, 2016. 270: p. 142-6.
http://www.ncbi.nlm.nih.gov/pubmed/27490302
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09-02-2016 11:54 PM
A systematic mutagenesis-driven strategy for site-resolved NMR studies of supramolecular assemblies
A systematic mutagenesis-driven strategy for site-resolved NMR studies of supramolecular assemblies
Abstract Obtaining sequence-specific assignments remains a major bottleneck in solution NMR investigations of supramolecular structure, dynamics and interactions. Here we demonstrate that resonance assignment of methyl probes in high molecular weight protein assemblies can be efficiently achieved by combining fast NMR experiments, residue-type-specific isotope-labeling and automated site-directed mutagenesis. The utility of this general and straightforward strategy is demonstrated through...
In situ light-driven pH modulation for NMR studies
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