Related ArticlesRefined Crystal Structure of Samia cynthia ricini Silk Fibroin Revealed by Solid-State NMR Investigations.
Biomacromolecules. 2017 Jun 12;18(6):1965-1974
Authors: Asakura T, Nishimura A, Kametani S, Kawanishi S, Aoki A, Suzuki F, Kaji H, Naito A
Abstract
Samia cynthia ricini is one of the wild silkworms and its silk fibroin (SF) consists of alternatively repeating poly-l-alanine (PLA) sequences as crystalline domain and glycine-rich sequences as noncrystalline domain; the structure is similar to those of spider silk and other wild silkworm silks. In this paper, we proposed a new staggered model for the packing arrangement of the PLA sequence through the use of the Cambridge Serial Total Energy Package program and a comparison of the observed and calculated chemical shifts of the PLA sequence with the Gauge Including Projector Augmented Wave method. The new model was supported by the interatomic distance information from the cross peaks of Ala C? dipolar-assisted rotational resonance (DARR) spectrum of the PLA sequences in S. c. ricini SF fiber. In addition, three 13C NMR peaks observed in the ?-sheet region were assigned to the carbons with different environments in the same model, but not assigned to different ?-sheet structures.
[NMR paper] Packing arrangement of 13C selectively labeled sequence model peptides of Samia cynthia ricini silk fibroin fibers studied by solid-state NMR.
Packing arrangement of 13C selectively labeled sequence model peptides of Samia cynthia ricini silk fibroin fibers studied by solid-state NMR.
http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--www.rsc.org-images-entities-char_z_RSClogo.gif Related Articles Packing arrangement of 13C selectively labeled sequence model peptides of Samia cynthia ricini silk fibroin fibers studied by solid-state NMR.
Phys Chem Chem Phys. 2017 May 24;19(20):13379-13386
Authors: Asakura T, Miyazawa K, Tasei Y, Kametani S, Nakazawa Y, Aoki A, Naito A...
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[NMR paper] Characterization of water in hydrated Bombyx mori silk fibroin fiber and films by (2)H NMR relaxation and (13)C solid state NMR.
Characterization of water in hydrated Bombyx mori silk fibroin fiber and films by (2)H NMR relaxation and (13)C solid state NMR.
http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--linkinghub.elsevier.com-ihub-images-PubMedLink.gif Related Articles Characterization of water in hydrated Bombyx mori silk fibroin fiber and films by (2)H NMR relaxation and (13)C solid state NMR.
Acta Biomater. 2017 Mar 01;50:322-333
Authors: Asakura T, Isobe K, Kametani S, Ukpebor OT, Silverstein MC, Boutis GS
Abstract
The...
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[NMR paper] Glycerin-Induced Conformational Changes in Bombyx mori Silk Fibroin Film Monitored by (13)C CP/MAS NMR and ¹H DQMAS NMR.
Glycerin-Induced Conformational Changes in Bombyx mori Silk Fibroin Film Monitored by (13)C CP/MAS NMR and ¹H DQMAS NMR.
http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/https:--www.ncbi.nlm.nih.gov-corehtml-pmc-pmcgifs-pubmed-pmc.gif Related Articles Glycerin-Induced Conformational Changes in Bombyx mori Silk Fibroin Film Monitored by (13)C CP/MAS NMR and ¹H DQMAS NMR.
Int J Mol Sci. 2016 Sep 09;17(9):
Authors: Asakura T, Endo M, Hirayama M, Arai H, Aoki A, Tasei Y
Abstract
In order to improve the stiff and...
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[NMR paper] Effect of pH and copper(II) on the conformation transitions of silk fibroin based on
Effect of pH and copper(II) on the conformation transitions of silk fibroin based on EPR, NMR, and Raman spectroscopy.
Related Articles Effect of pH and copper(II) on the conformation transitions of silk fibroin based on EPR, NMR, and Raman spectroscopy.
Biochemistry. 2004 Sep 28;43(38):11932-41
Authors: Zong XH, Zhou P, Shao ZZ, Chen SM, Chen X, Hu BW, Deng F, Yao WH
Much attention has been paid to the natural mechanism of silkworm spinning due to the impressive mechanical properties of the natural fibers. Our results in the present work show...
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[NMR paper] Structural analysis of Bombyx mori silk fibroin peptides with formic acid treatment u
Structural analysis of Bombyx mori silk fibroin peptides with formic acid treatment using high-resolution solid-state 13C NMR spectroscopy.
Related Articles Structural analysis of Bombyx mori silk fibroin peptides with formic acid treatment using high-resolution solid-state 13C NMR spectroscopy.
Biomacromolecules. 2004 Sep-Oct;5(5):1763-9
Authors: Yao J, Ohgo K, Sugino R, Kishore R, Asakura T
Bombyx mori silk fibroin fiber is a fibrous protein produced by the silkworm at room temperature and from an aqueous solution whose primary structure is...
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[NMR paper] Structural role of tyrosine in Bombyx mori silk fibroin, studied by solid-state NMR a
Structural role of tyrosine in Bombyx mori silk fibroin, studied by solid-state NMR and molecular mechanics on a model peptide prepared as silk I and II.
Related Articles Structural role of tyrosine in Bombyx mori silk fibroin, studied by solid-state NMR and molecular mechanics on a model peptide prepared as silk I and II.
Magn Reson Chem. 2004 Feb;42(2):258-66
Authors: Asakura T, Suita K, Kameda T, Afonin S, Ulrich AS
The influence of the bulky and H-bonding Tyr side-chain on its Ala- and Gly-rich environment in Bombyx mori silk fibroin was...
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[NMR paper] Solid-state NMR determination of the secondary structure of Samia cynthia ricini silk
Solid-state NMR determination of the secondary structure of Samia cynthia ricini silk.
Related Articles Solid-state NMR determination of the secondary structure of Samia cynthia ricini silk.
Nature. 2000 Jun 29;405(6790):1077-9
Authors: van Beek JD, Beaulieu L, Schäfer H, Demura M, Asakura T, Meier BH
Silks are fibrous proteins that form heterogeneous, semi-crystalline solids. Silk proteins have a variety of physical properties reflecting their range of functions. Spider dragline silk, for example, has high tensile strength and elasticity,...
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[NMR paper] NMR investigations of protein-carbohydrate interactions: refined three-dimensional st
NMR investigations of protein-carbohydrate interactions: refined three-dimensional structure of the complex between hevein and methyl beta-chitobioside.
Related Articles NMR investigations of protein-carbohydrate interactions: refined three-dimensional structure of the complex between hevein and methyl beta-chitobioside.
Glycobiology. 1998 Jun;8(6):569-77
Authors: Asensio JL, Cañada FJ, Bruix M, González C, Khiar N, Rodríguez-Romero A, Jiménez-Barbero J
The specific interaction of hevein with GlcNAc-containing oligosaccharides has been...
Refined Crystal Structure of Samia cynthia ricini Silk Fibroin Revealed by Solid-State NMR Investigations.
Linear Mode
