Related ArticlesReduced BPTI is collapsed. A pulsed field gradient NMR study of unfolded and partially folded bovine pancreatic trypsin inhibitor.
Protein Sci. 1997 Sep;6(9):1985-92
Authors: Pan H, Barany G, Woodward C
Pulsed field gradient NMR was used to measure the hydrodynamic behavior of unfolded variants of bovine pancreatic trypsin inhibitor (BPTI). The unfolded BPTI species studied were [R]Abu, at pH 4.5 and pH 2.5, and unfolded [14-38]Abu, at pH 2.5. These were prepared by chemical synthesis. [R]Abu is a model for reduced BPTI; all cysteine residues are replaced by alpha-amino-n-butyric acid (Abu). [14-38]Abu retains cysteines 14 and 38, which form a disulfide bond, while the other cysteine residues are replaced by Abu. In the PFG experiments, the diffusion coefficient is measured as a function of protein concentration, and the value of D degree -the diffusion coefficient extrapolated to infinite dilution-is determined. From D degree, a value of the hydrodynamic radius. Rh, is computed from the Stokes-Einstein relationship. At pH 4.5, [R]Abu has an Rh value significantly less than the value calculated for a random coil, while at pH 2.5 the experimental Rh value is the same as for a random coil. In view of the changes in NMR detected structure of [R]Abu at pH 4.5 versus pH 2.5 (Pan H, Barbar E, Barany G, Woodward C. 1995. Extensive non-random structure in reduced and unfolded bovine pancreatic trypsin inhibitor. Biochemistry 34:13974-13981), the collapse of reduced BPTI at pH 4.5 may be associated with the formation of non-native hydrophobic clusters of pairs of side chains one to three amino acids apart in sequence. The diffusion constant of [14-38]Abu was also measured at pH 4.5, where the protein is partially folded. An increase in hydrodynamic radius of partially folded [14-38]Abu, relative to native BPTI, is similar to the increase in radius of gyration measured for other proteins under "molten globule" conditions.
Translational Diffusion of Macromolecular Assemblies Measured Using Transverse-Relaxation-Optimized Pulsed Field Gradient NMR
Translational Diffusion of Macromolecular Assemblies Measured Using Transverse-Relaxation-Optimized Pulsed Field Gradient NMR
Reto Horst, Arthur L. Horwich and Kurt Wu?thrich
http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jacsat/0/jacsat.ahead-of-print/ja206531c/aop/images/medium/ja-2011-06531c_0003.gif
Journal of the American Chemical Society
DOI: 10.1021/ja206531c
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Probing Microscopic Architecture of Opaque Heterogeneous Systems Using Double-Pulsed-Field-Gradient NMR
Probing Microscopic Architecture of Opaque Heterogeneous Systems Using Double-Pulsed-Field-Gradient NMR
Noam Shemesh, Tal Adiri and Yoram Cohen
http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jacsat/0/jacsat.ahead-of-print/ja200303h/aop/images/medium/ja-2011-00303h_0005.gif
Journal of the American Chemical Society
DOI: 10.1021/ja200303h
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[NMR paper] Slow diffusion of macromolecular assemblies by a new pulsed field gradient NMR method
Slow diffusion of macromolecular assemblies by a new pulsed field gradient NMR method.
Related Articles Slow diffusion of macromolecular assemblies by a new pulsed field gradient NMR method.
J Am Chem Soc. 2003 Mar 5;125(9):2541-5
Authors: Ferrage F, Zoonens M, Warschawski DE, Popot JL, Bodenhausen G
The translational diffusion coefficient of an integral membrane protein/surfactant complex has been measured using a novel pulsed field gradient NMR method. In this new approach, the information about the localization of the molecules is...
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[NMR paper] A pulsed field gradient NMR study of the aggregation and hydration of parvalbumin.
A pulsed field gradient NMR study of the aggregation and hydration of parvalbumin.
http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--linkinghub.elsevier.com-ihub-images-PubMedLink.gif Related Articles A pulsed field gradient NMR study of the aggregation and hydration of parvalbumin.
Biophys Chem. 1997 Apr 22;65(2-3):179-87
Authors: Price WS, Nara M, Arata Y
Pulsed field gradient NMR is a convenient alternative to traditional methods for measuring diffusion of biological macromolecules. In the present study, pulsed field gradient NMR was...
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[NMR paper] A pulsed-field gradient NMR study of bovine pancreatic trypsin inhibitor self-associa
A pulsed-field gradient NMR study of bovine pancreatic trypsin inhibitor self-association.
http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--pubs.acs.org-images-acspubs.jpg Related Articles A pulsed-field gradient NMR study of bovine pancreatic trypsin inhibitor self-association.
Biochemistry. 1997 Mar 18;36(11):3383-8
Authors: Ilyina E, Roongta V, Pan H, Woodward C, Mayo KH
Previous studies have produced conflicting interpretations regarding the aggregation state of BPTI in solution. Here, pulsed-field gradient NMR self-association...
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08-22-2010 03:31 PM
[NMR paper] A pulsed field gradient NMR study of the aggregation and hydration of parvalbumin.
A pulsed field gradient NMR study of the aggregation and hydration of parvalbumin.
http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--linkinghub.elsevier.com-ihub-images-PubMedLink.gif Related Articles A pulsed field gradient NMR study of the aggregation and hydration of parvalbumin.
Biophys Chem. 1997 Apr 22;65(2-3):179-87
Authors: Price WS, Nara M, Arata Y
Pulsed field gradient NMR is a convenient alternative to traditional methods for measuring diffusion of biological macromolecules. In the present study, pulsed field gradient NMR was...
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08-22-2010 03:03 PM
[NMR paper] A pulsed-field gradient NMR study of bovine pancreatic trypsin inhibitor self-associa
A pulsed-field gradient NMR study of bovine pancreatic trypsin inhibitor self-association.
http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--pubs.acs.org-images-acspubs.jpg Related Articles A pulsed-field gradient NMR study of bovine pancreatic trypsin inhibitor self-association.
Biochemistry. 1997 Mar 18;36(11):3383-8
Authors: Ilyina E, Roongta V, Pan H, Woodward C, Mayo KH
Previous studies have produced conflicting interpretations regarding the aggregation state of BPTI in solution. Here, pulsed-field gradient NMR self-association...
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[NMR paper] Measuring protein self-association using pulsed-field-gradient NMR spectroscopy: appl
Measuring protein self-association using pulsed-field-gradient NMR spectroscopy: application to myosin light chain 2.
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J Biomol NMR. 1995 Nov;6(3):321-8
Authors: Dingley AJ, Mackay JP, Chapman BE, Morris MB, Kuchel PW, Hambly BD, King GF
At the millimolar concentrations required for structural studies, NMR spectra of the calcium-binding protein myosin light chain 2 (MLC2) showed resonance line widths indicative...