Hydroxyapatite (HAP) is the main mineral component of teeth. It is well-known that several salivary proteins and peptides bind strongly to HAP to regulate crystal growth. Interactions between a peptide derived from the N-terminal fragment of the salivary protein statherin and HAP were measured utilizing rotational-echo double-resonance (REDOR) nuclear magnetic resonance (NMR). The REDOR measurement from the side chain of the salivary peptide to the HAP surface is complicated by two effects: a possible additional dipolar coupling to a phosphorylated side chain and the potential proximity of phosphorus atoms to each other, resulting in a homonuclear dipolar interaction. Both of these effects were addressed, and the smallest model applicable to our system includes the nitrogen-15 (15N) spin in the lysine side chain and two phosphorus-31 (31P) spins, at least one of which must be from the surface phosphates of the HAP.
Solid-state and unilateral NMR study of deterioration of a Dead Sea Scroll fragment.
Solid-state and unilateral NMR study of deterioration of a Dead Sea Scroll fragment.
Solid-state and unilateral NMR study of deterioration of a Dead Sea Scroll fragment.
Anal Bioanal Chem. 2011 Jul 30;
Authors: Masic A, Chierotti MR, Gobetto R, Martra G, Rabin I, Coluccia S
Unilateral and solid-state nuclear magnetic resonance (NMR) analyses were performed on a parchment fragment of the Dead Sea Scroll (DSS). The analyzed sample belongs to the collection of non-inscribed and nontreated fragments of known archaeological provenance from the John...
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08-02-2011 11:40 AM
(13)C/(15)N-(19)F Intermolecular REDOR NMR Study of the Interaction of TAR RNA with T
(13)C/(15)N-(19)F Intermolecular REDOR NMR Study of the Interaction of TAR RNA with Tat Peptides.
(13)C/(15)N-(19)F Intermolecular REDOR NMR Study of the Interaction of TAR RNA with Tat Peptides.
J Am Chem Soc. 2010 Nov 24;
Authors: Huang W, Varani G, Drobny GP
The complex of the HIV TAR RNA with the viral regulatory protein Tat is of considerable interest, but the plasticity of this interaction has made it impossible so far to establish the structure of that complex. In order to explore a new approach to obtain structural information on...
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11-26-2010 05:32 PM
13C/15N-19F Intermolecular REDOR NMR Study of the Interaction of TAR RNA with Tat Pep
13C/15N-19F Intermolecular REDOR NMR Study of the Interaction of TAR RNA with Tat Peptides
Wei Huang, Gabriele Varani and Gary P. Drobny
http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jacsat/0/jacsat.ahead-of-print/ja1051439/aop/images/medium/ja-2010-051439_0005.gif
Journal of the American Chemical Society
DOI: 10.1021/ja1051439
http://feeds.feedburner.com/~ff/acs/jacsat?d=yIl2AUoC8zA
http://feeds.feedburner.com/~r/acs/jacsat/~4/bKQhcXWaqW0
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11-25-2010 07:22 AM
[NMR paper] Structure and dynamics of hydrated statherin on hydroxyapatite as determined by solid
Structure and dynamics of hydrated statherin on hydroxyapatite as determined by solid-state NMR.
Related Articles Structure and dynamics of hydrated statherin on hydroxyapatite as determined by solid-state NMR.
Biochemistry. 2001 Dec 25;40(51):15451-5
Authors: Long JR, Shaw WJ, Stayton PS, Drobny GP
Proteins directly control the nucleation and growth of biominerals, but the details of molecular recognition at the protein-biomineral interface remain poorly understood. The elucidation of recognition mechanisms at this interface may provide...
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11-19-2010 08:44 PM
[NMR paper] Dynamics of trimethoprim bound to dihydrofolate reductase--a deuterium NMR study.
Dynamics of trimethoprim bound to dihydrofolate reductase--a deuterium NMR study.
http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--linkinghub.elsevier.com-ihub-images-PubMedLink.gif Related Articles Dynamics of trimethoprim bound to dihydrofolate reductase--a deuterium NMR study.
Solid State Nucl Magn Reson. 1996 Dec;7(3):193-201
Authors: Yang QX, Huang FY, Lin TH, Gelbaum L, Howell EE, Huang TH
We have employed deuterium NMR techniques to determine the dynamics of trimethoprim (TMP) in a binary complex with dihydrofolate reductase...
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08-22-2010 02:20 PM
[NMR paper] NMR study of the reconstitution of the beta-sheet of thioredoxin by fragment compleme
NMR study of the reconstitution of the beta-sheet of thioredoxin by fragment complementation.
Related Articles NMR study of the reconstitution of the beta-sheet of thioredoxin by fragment complementation.
Proteins. 1995 May;22(1):41-4
Authors: Tasayco ML, Chao K
The study of complementary protein fragments is thought to be generally useful to identify early folding intermediates. A prerequisite for these studies is the reconstitution of the native-like structure by fragment complementation. Structural analysis of the complementation of the...
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08-22-2010 03:41 AM
[NMR paper] 13C NMR study of the mode of interaction in solution of the B fragment of staphylococ
13C NMR study of the mode of interaction in solution of the B fragment of staphylococcal protein A and the Fc fragments of mouse immunoglobulin G.
http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--linkinghub.elsevier.com-ihub-images-PubMedLink.gif Related Articles 13C NMR study of the mode of interaction in solution of the B fragment of staphylococcal protein A and the Fc fragments of mouse immunoglobulin G.
FEBS Lett. 1993 Aug 9;328(1-2):49-54
Authors: Kato K, Gouda H, Takaha W, Yoshino A, Matsunaga C, Arata Y
The mode of interaction of...
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[NMR paper] 31P-NMR assignment and conformational study of NADPH bound to Lactobacillus casei dih
31P-NMR assignment and conformational study of NADPH bound to Lactobacillus casei dihydrofolate reductase based on two-dimensional 1H-31P-heteronuclear and 1H-detected 1H-31P-shift-correlation experiments.
http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--www3.interscience.wiley.com-aboutus-images-wiley_interscience_pubmed_logo_FREE_120x27.gif Related Articles 31P-NMR assignment and conformational study of NADPH bound to Lactobacillus casei dihydrofolate reductase based on two-dimensional 1H-31P-heteronuclear and 1H-detected 1H-31P-shift-correlation experiments.
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A REDOR NMR study of a phosphorylated statherin fragment bound to hydroxyapatite crystals.
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