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Default Reconstruction of non-uniformly sampled five-dimensional NMR spectra by signal separation algorithm.

Reconstruction of non-uniformly sampled five-dimensional NMR spectra by signal separation algorithm.

Related Articles Reconstruction of non-uniformly sampled five-dimensional NMR spectra by signal separation algorithm.

J Biomol NMR. 2017 Feb 28;:

Authors: Kosi?ski K, Stanek J, Górka MJ, ?erko S, Ko?mi?ski W

Abstract
A method for five-dimensional spectral reconstruction of non-uniformly sampled NMR data sets is proposed. It is derived from the previously published signal separation algorithm, with major alterations to avoid unfeasible processing of an entire five-dimensional spectrum. The proposed method allows credible reconstruction of spectra from as little as a few hundred data points and enables sensitive resonance detection in experiments with a high dynamic range of peak intensities. The efficiency of the method is demonstrated on two high-resolution spectra for rapid sequential assignment of intrinsically disordered proteins, namely 5D HN(CA)CONH and 5D (HACA)CON(CO)CONH.


PMID: 28243768 [PubMed - as supplied by publisher]



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