[NMR paper] Recombinant Production, NMR Solution Structure, and Membrane Interaction of the Ph?1? Toxin, a TRPA1 Modulator from the Brazilian Armed Spider Phoneutria nigriventer
Recombinant Production, NMR Solution Structure, and Membrane Interaction of the Ph?1? Toxin, a TRPA1 Modulator from the Brazilian Armed Spider Phoneutria nigriventer
Ph?1? (PnTx3-6) is a neurotoxin from the spider Phoneutria nigriventer venom, originally identified as an antagonist of two ion channels involved in nociception: N-type voltage-gated calcium channel (Ca(V)2.2) and TRPA1. In animal models, Ph?1? administration reduces both acute and chronic pain. Here, we report the efficient bacterial expression system for the recombinant production of Ph?1? and its ^(15)N-labeled analogue. Spatial structure and dynamics of Ph?1? were determined via NMR...
[NMR paper] Presence of beta-Turn Structure in Recombinant Spider Silk Dissolved in Formic Acid Revealed with NMR
Presence of beta-Turn Structure in Recombinant Spider Silk Dissolved in Formic Acid Revealed with NMR
Spider dragline silk is a biopolymer with excellent mechanical properties. The development of recombinant spider silk protein (RSP)-based materials with these properties is desirable. Formic acid (FA) is a spinning solvent for regenerated Bombyx mori silk fiber with excellent mechanical properties. To use FA as a spinning solvent for RSP with the sequence of major ampullate spider silk protein from Araneus diadematus, we determined the conformation of RSP in FA using solution NMR to...
nmrlearner
Journal club
0
01-22-2022 02:38 AM
Successful refolding and NMR structure of rMagi3: A disulfide-rich insecticidal spider toxin
Successful refolding and NMR structure of rMagi3: A disulfide-rich insecticidal spider toxin
Abstract
The need for molecules with high specificity against noxious insects leads the search towards spider venoms that have evolved highly selective toxins for insect preys. In this respect, spiders as a highly diversified group of almost exclusive insect predators appear to possess infinite potential for the discovery of novel insect-selective toxins. In 2003, a group of toxins was isolated from the spider Macrothele gigas and the amino acid sequence was reported. We obtained, by molecular...
nmrlearner
Journal club
0
02-20-2018 10:04 AM
Successful refolding and NMR structure of rMagi3: a disulfide-rich insecticidal spider toxin
Successful refolding and NMR structure of rMagi3: a disulfide-rich insecticidal spider toxin
Abstract
The need for molecules with high specificity against noxious insects leads the search towards spider venoms that have evolved highly selective toxins for insect preys. In this respect, spiders as a highly diversified group of almost exclusive insect predators appear to possess infinite potential for the discovery of novel insect-selective toxins. In 2003, a group of toxins was isolated from the spider Macrothele gigas and the amino acid sequence was reported. We obtained, by molecular...
nmrlearner
Journal club
0
01-04-2018 08:45 AM
[NMR paper] Successful refolding and NMR structure of rMagi3: A disulfide-rich insecticidal spider toxin.
Successful refolding and NMR structure of rMagi3: A disulfide-rich insecticidal spider toxin.
Related Articles Successful refolding and NMR structure of rMagi3: A disulfide-rich insecticidal spider toxin.
Protein Sci. 2017 Dec 16;:
Authors: Titaux-Delgado G, Carrillo E, Mendoza A, Mayorga-Flores M, Escobedo-González FC, Cano-Sánchez P, López-Vera E, Corzo G, Del Rio-Portilla F
Abstract
The need for molecules with high specificity against noxious insects leads the search towards spider venoms that have evolved highly...
nmrlearner
Journal club
0
12-19-2017 11:31 AM
Successful refolding and NMR structure of rMagi3: A disulfide-rich insecticidal spider toxin
Successful refolding and NMR structure of rMagi3: A disulfide-rich insecticidal spider toxin
Abstract
The need for molecules with high specificity against noxious insects leads the search towards spider venoms that have evolved highly selective toxins for insect preys. In this respect, spiders as a highly diversified group of almost exclusive insect predators appear to possess infinite potential for the discovery of novel insect-selective toxins. In 2003 a group of toxins was isolated from the spider Macrothele gigas and the amino acid sequence was reported. We obtained, by molecular...
nmrlearner
Journal club
0
12-17-2017 10:34 AM
[NMR paper] NMR solution structure and membrane interaction of the N-terminal sequence (1-30) of
NMR solution structure and membrane interaction of the N-terminal sequence (1-30) of the bovine prion protein.
Related Articles NMR solution structure and membrane interaction of the N-terminal sequence (1-30) of the bovine prion protein.
Biochemistry. 2004 Nov 30;43(47):14940-7
Authors: Biverståhl H, Andersson A, Gräslund A, Mäler L
The structure and membrane interaction of the N-terminal sequence (1-30) of the bovine prion protein (bPrPp) has been investigated by NMR spectroscopy in phospholipid membrane mimetic systems. CD spectroscopy...
nmrlearner
Journal club
0
11-24-2010 10:03 PM
[NMR paper] Three-dimensional structure of the RGD-containing snake toxin albolabrin in solution,
Three-dimensional structure of the RGD-containing snake toxin albolabrin in solution, based on 1H NMR spectroscopy and simulated annealing calculations.
Related Articles Three-dimensional structure of the RGD-containing snake toxin albolabrin in solution, based on 1H NMR spectroscopy and simulated annealing calculations.
Int J Pept Protein Res. 1996 Sep;48(3):220-8
Authors: Smith KJ, Jaseja M, Lu X, Williams JA, Hyde EI, Trayer IP
Albolabrin is a snake toxin that contains a RGD-(Arg-Gly-Asp) sequence motif and competes with fibrinogen to bind...
Recombinant Production, NMR Solution Structure, and Membrane Interaction of the Ph?1? Toxin, a TRPA1 Modulator from the Brazilian Armed Spider Phoneutria nigriventer
Linear Mode
