Recent contributions from solid-state NMR to the understanding of membrane protein structure and function.

		BioNMR > Educational resources > Journal club
Recent contributions from solid-state NMR to the understanding of membrane protein structure and function.

Webservers

NMR processing:

MDD

NMR assignment:

Backbone:

Side-chains:

NOEs:

Structure from NMR restraints:

Ab initio:

Fragment-based:

Rosetta-NMR (Robetta)

Template-based:

GeNMR

I-TASSER

Refinement:

Amber

Structure from chemical shifts:

Fragment-based:

Homology-based:

Torsion angles from chemical shifts:

Preditor

TALOS

Promega- Proline

Secondary structure from chemical shifts:

CSI (via RCI server)

TALOS

MICS caps, β-turns

d2D

PECAN

Flexibility from chemical shifts:

RCI

Interactions from chemical shifts:

HADDOCK

Chemical shifts re-referencing:

NMR model quality:

NOEs, other restraints:

Chemical shifts:

RDCs:

Pseudocontact shifts:

Protein geomtery:

SAVES2 or SAVES4

Quality Control Check

NMR spectrum prediction:

FANDAS

MestReS

V-NMR

Flexibility from structure:

Backbone S2

Methyl S2

B-factor

Molecular dynamics:

Gromacs

Amber

Antechamber

Chemical shifts prediction:

From structure:

Shiftx2

Sparta+

Camshift

CH3shift- Methyl

ArShift- Aromatic

ShiftS

Proshift

PPM

CheShift-2- Cα

From sequence:

Shifty

Camcoil

Poulsen_rc_CS

Disordered proteins:

MAXOCC

Format conversion & validation:

CCPN

From NMR-STAR 3.1

Validate NMR-STAR 3.1

NMR sample preparation:

Protein disorder:

DisMeta

Protein solubility:

Isotope labeling:

Solid-state NMR:

Thread Tools

Search this Thread

Rate Thread

Display Modes

08-25-2011, 04:10 PM

nmrlearner

Senior Member

Join Date: Jan 2005

Posts: 23,777

Points: 193,617, Level: 100

Level up: 0%, 0 Points needed

Activity: 50.7%

Last Achievements

Award-Showcase

NMR Credits: 0

NMR Points: 193,617

Downloads: 0

Uploads: 0

Recent contributions from solid-state NMR to the understanding of membrane protein structure and function.

Recent contributions from solid-state NMR to the understanding of membrane protein structure and function.

Recent contributions from solid-state NMR to the understanding of membrane protein structure and function.

Curr Opin Chem Biol. 2011 Aug 19;

Authors: Judge PJ, Watts A

Abstract
The plasma membrane functions as a semi-permeable barrier, defining the interior (or cytoplasm) of an individual cell. This highly dynamic and complex macromolecular assembly comprises predominantly lipids and proteins held together by entropic forces and provide the interface through which a cell interacts with its immediate environment. The extended sheet-like bilayer structure formed by the phospholipids is a highly adaptable platform whose structure and composition may be tuned to provide specialised functionality. Although a number of biophysical techniques including X-ray crystallography have been used to determine membrane protein structures, these methods are unable to replicate and accommodate the complexity and diversity of natural membranes. Solid state NMR (ssNMR) is a versatile method for structural biology and can be used to provide new insights into the structures of membrane components and their mutual interactions. The extensive variety of sample forms amenable for study by ssNMR, allows data to be collected from proteins in conditions that more faithfully resemble those of native environment, and therefore is much closer to a functional state.

PMID: 21862384 [PubMed - as supplied by publisher]

Source: PubMed

Did you find this post helpful?

Similar Threads
Thread	Thread Starter	Forum	Replies	Last Post
Solid-State NMR on a Large Multidomain Integral Membrane Protein: The Outer Membrane Protein Assembly Factor BamA. Solid-State NMR on a Large Multidomain Integral Membrane Protein: The Outer Membrane Protein Assembly Factor BamA. Solid-State NMR on a Large Multidomain Integral Membrane Protein: The Outer Membrane Protein Assembly Factor BamA. J Am Chem Soc. 2011 Mar 1; Authors: Renault M, Bos MP, Tommassen J, Baldus M Multidomain proteins constitute a large part of prokaryotic and eukaryotic proteomes and play fundamental roles in various physiological processes. However, their structural characterization is challenging because of their large size and...	nmrlearner	Journal club	0	03-03-2011 12:34 PM
Solid-State NMR on a Large Multidomain Integral Membrane Protein: The Outer Membrane Protein Assembly Factor BamA Solid-State NMR on a Large Multidomain Integral Membrane Protein: The Outer Membrane Protein Assembly Factor BamA Marie Renault, Martine P. Bos, Jan Tommassen and Marc Baldus http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jacsat/0/jacsat.ahead-of-print/ja109469c/aop/images/medium/ja-2010-09469c_0004.gif Journal of the American Chemical Society DOI: 10.1021/ja109469c http://feeds.feedburner.com/~ff/acs/jacsat?d=yIl2AUoC8zA http://feeds.feedburner.com/~r/acs/jacsat/~4/9XN1qiW-S-I	nmrlearner	Journal club	0	03-02-2011 02:01 AM
Pair Distribution Function Analysis and Solid State NMR Studies of Silicon Electrodes for Lithium Ion Batteries: Understanding the (De)lithiation Mechanisms Pair Distribution Function Analysis and Solid State NMR Studies of Silicon Electrodes for Lithium Ion Batteries: Understanding the (De)lithiation Mechanisms Baris Key, Mathieu Morcrette, Jean-Marie Tarascon and Clare P. Grey http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jacsat/0/jacsat.ahead-of-print/ja108085d/aop/images/medium/ja-2010-08085d_0006.gif Journal of the American Chemical Society DOI: 10.1021/ja108085d http://feeds.feedburner.com/~ff/acs/jacsat?d=yIl2AUoC8zA http://feeds.feedburner.com/~r/acs/jacsat/~4/WYK7o0vOALs	nmrlearner	Journal club	0	12-21-2010 02:14 AM
[NMR paper] Membrane protein structure determination using solid-state NMR. Membrane protein structure determination using solid-state NMR. Related Articles Membrane protein structure determination using solid-state NMR. Methods Mol Biol. 2004;278:403-73 Authors: Watts A, Straus SK, Grage SL, Kamihira M, Lam YH, Zhao X Solid-state NMR is emerging as a method for resolving structural information for large biomolecular complexes, such as membrane-embedded proteins. In principle, there is no molecular weight limit to the use of the approach, although the complexity and volume of data is still outside complete assignment...	nmrlearner	Journal club	0	11-24-2010 09:25 PM
[NMR paper] A solid-state NMR index of helical membrane protein structure and topology. A solid-state NMR index of helical membrane protein structure and topology. Related Articles A solid-state NMR index of helical membrane protein structure and topology. J Magn Reson. 2000 May;144(1):150-5 Authors: Marassi FM, Opella SJ The secondary structure and topology of membrane proteins can be described by inspection of two-dimensional (1)H-(15)N dipolar coupling/(15)N chemical shift polarization inversion spin exchange at the magic angle spectra obtained from uniformly (15)N-labeled samples in oriented bilayers. The characteristic...	nmrlearner	Journal club	0	11-18-2010 09:15 PM
[NMR paper] Membrane protein structure determination by solid state NMR. Membrane protein structure determination by solid state NMR. Related Articles Membrane protein structure determination by solid state NMR. Nat Prod Rep. 1999 Aug;16(4):419-23 Authors: Watts A, Burnett IJ, Glaubitz C, Gröbner G, Middleton DA, Spooner PJ, Watts JA, Williamson PT	nmrlearner	Journal club	0	11-18-2010 08:31 PM
[NMR paper] Membrane protein structure: the contribution and potential of novel solid state NMR a Membrane protein structure: the contribution and potential of novel solid state NMR approaches. Related Articles Membrane protein structure: the contribution and potential of novel solid state NMR approaches. Mol Membr Biol. 1995 Jul-Sep;12(3):233-46 Authors: Watts A, Ulrich AS, Middleton DA Alternative methods for describing molecular detail for large integral membrane proteins are required in the absence of routine crystallographic approaches. Novel solid state NMR methods, devised for the study of large molecular assemblies, are now finding...	nmrlearner	Journal club	0	08-22-2010 03:41 AM
[NMR paper] Solid-state NMR approaches for studying membrane protein structure. Solid-state NMR approaches for studying membrane protein structure. http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--arjournals.annualreviews.org-images-AnnualReviews100x25.gif Related Articles Solid-state NMR approaches for studying membrane protein structure. Annu Rev Biophys Biomol Struct. 1992;21:25-47 Authors: Smith SO, Peersen OB	nmrlearner	Journal club	0	08-21-2010 11:41 PM

« Previous Thread | Next Thread »

Posting Rules
You may not post new threads You may not post replies You may not post attachments You may not edit your posts BB code is On Smilies are On [IMG] code is On HTML code is On Trackbacks are Off Pingbacks are Off Refbacks are Off