Non-invasive and real-time recording of processes in living cells has been limited to detection of small cellular components such as soluble proteins and metabolites. Here we report a multiphase NMR approach using Magic-Angle Spinning NMR to synchronously follow microbial processes of fermentation, lipid metabolism and structural dynamic changes in live microalgae cells. Chlamydomonas reinhardtii green algae were highly concentrated, introducing dark fermentation and anoxia conditions....
[NMR paper] Real-Time NMR Spectroscopy for Studying Metabolism.
Real-Time NMR Spectroscopy for Studying Metabolism.
http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--media.wiley.com-assets-7388-69-wiley-full-text.png http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/https:--www.ncbi.nlm.nih.gov-corehtml-pmc-pmcgifs-pubmed-pmc.png Related Articles Real-Time NMR Spectroscopy for Studying Metabolism.
Angew Chem Int Ed Engl. 2020 02 03;59(6):2304-2308
Authors: Alshamleh I, Krause N, Richter C, Kurrle N, Serve H, Günther UL, Schwalbe H
Abstract
Current...
nmrlearner
Journal club
0
01-15-2021 04:25 PM
[NMR paper] Active metabolism unmasks functional protein-protein interactions in real time in-cell NMR.
Active metabolism unmasks functional protein-protein interactions in real time in-cell NMR.
Related Articles Active metabolism unmasks functional protein-protein interactions in real time in-cell NMR.
Commun Biol. 2020 May 21;3(1):249
Authors: Breindel L, Burz DS, Shekhtman A
Abstract
Protein-protein interactions, PPIs, underlie most cellular processes, but many PPIs depend on a particular metabolic state that can only be observed in live, actively metabolizing cells. Real time in-cell NMR spectroscopy, RT-NMR, utilizes a...
nmrlearner
Journal club
0
05-24-2020 05:50 AM
[NMR paper] Real-Time Monitoring of Cancer Cell Metabolism and Effects of an Anticancer Agent using 2D In-Cell NMR Spectroscopy.
Real-Time Monitoring of Cancer Cell Metabolism and Effects of an Anticancer Agent using 2D In-Cell NMR Spectroscopy.
http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--media.wiley.com-assets-2250-98-WileyOnlineLibrary_FullTextOnline_120x27.gif Related Articles Real-Time Monitoring of Cancer Cell Metabolism and Effects of an Anticancer Agent using 2D In-Cell NMR Spectroscopy.
Angew Chem Int Ed Engl. 2015 Mar 5;
Authors: Wen H, An YJ, Xu WJ, Kang KW, Park S
Abstract
Altered metabolism is a critical part of...
nmrlearner
Journal club
0
03-11-2015 09:59 PM
Real-time cardiac metabolism assessed with hyperpolarized [1-13C]acetate in a large-animal model
From The DNP-NMR Blog:
Real-time cardiac metabolism assessed with hyperpolarized acetate in a large-animal model
Flori, A., et al., Real-time cardiac metabolism assessed with hyperpolarized acetate in a large-animal model. Contrast Media & Molecular Imaging, 2014: p. n/a-n/a.
http://dx.doi.org/10.1002/cmmi.1618
nmrlearner
News from NMR blogs
0
01-14-2015 04:22 PM
Progression of NMR studies of membrane-active peptides from lipid bilayers to live cells
Progression of NMR studies of membrane-active peptides from lipid bilayers to live cells
Publication date: Available online 27 December 2014
Source:Journal of Magnetic Resonance</br>
Author(s): M.-A. Sani , F. Separovic</br>
Understanding the structure of membrane-active peptides faces many challenges associated with the development of appropriate model membrane systems as the peptide structure depends strongly on the lipid environment. This perspective provides a brief overview of the approach taken to study antimicrobial and amyloid peptides in phospholipid...
[NMR paper] Ultrafast Two-Dimensional NMR Relaxometry for Investigating Molecular Processes in Real Time.
Ultrafast Two-Dimensional NMR Relaxometry for Investigating Molecular Processes in Real Time.
http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--media.wiley.com-assets-2250-98-WileyOnlineLibrary-Button_120x27px_FullText.gif Related Articles Ultrafast Two-Dimensional NMR Relaxometry for Investigating Molecular Processes in Real Time.
Chemphyschem. 2014 Mar 13;
Authors: Ahola S, Telkki VV
Abstract
Nuclear spin-lattice (T1 ) and spin-spin (T2 ) relaxation times provide versatile information about the dynamics and...