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Default Real-time NMR monitoring of protein-folding kinetics by a recycle flow system for temperature jump.

Real-time NMR monitoring of protein-folding kinetics by a recycle flow system for temperature jump.

Real-time NMR monitoring of protein-folding kinetics by a recycle flow system for temperature jump.

Anal Chem. 2013 Sep 12;

Authors: Yamasaki K, Obara Y, Hasegawa M, Tanaka H, Yamasaki T, Wakuda T, Okada M, Kohzuma T

Abstract
An NMR method was developed that allows for real-time monitoring of reactions (on the order of seconds) induced by temperature jump. In a recycle flow system, heating and cooling baths were integrated, with the latter inside the NMR probe. A refolding reaction of ribonuclease A was triggered by rapid cooling and monitored by a series of NMR measurements over 12 s. Data were processed by principal component analysis, in which a factor related to the structural change with an exponential rate constant of 0.2-0.7 s-1 was successfully separated from factors related to baseline instability and/or noise. Temperature dependency of the rate constant revealed the entropy-driven formation of the transition state of the refolding reaction.


PMID: 24028433 [PubMed - as supplied by publisher]



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