[NMR paper] Reactivity characterization of bromoacetyl derivatized polyribosylribitol polysaccharide in Haemophilus influenzae type b for PedvaxHIB by NMR spectroscopy
Reactivity characterization of bromoacetyl derivatized polyribosylribitol polysaccharide in Haemophilus influenzae type b for PedvaxHIB by NMR spectroscopy
PedvaxHIB® is a highly purified capsular polysaccharide (polyribosylribitol phosphate or PRP) of Haemophilus influenzae type b (Hib) that is covalently linked to an outer membrane protein complex (OMPC) of Neisseria meningitidis pediatric vaccine. This Hib protein conjugate vaccine is very effective in protecting infants from invasive gram-negative bacterium Hib. Before conjugation of PRP to OMPC, PRP is first derivatized with butadiamine and bromoacetyl group which reacts with thiolated OMPC....
[NMR paper] Reactivity of Thiol-Rich Zn Sites in Diacylglycerol-Sensing PKC C1 Domain Probed by NMR Spectroscopy
Reactivity of Thiol-Rich Zn Sites in Diacylglycerol-Sensing PKC C1 Domain Probed by NMR Spectroscopy
Conserved homology 1 (C1) domains are peripheral zinc finger domains that are responsible for recruiting their host signaling proteins, including Protein Kinase C (PKC) isoenzymes, to diacylglycerol-containing lipid membranes. In this work, we investigated the reactivity of the C1 structural zinc sites, using the cysteine-rich C1B regulatory region of the PKC? isoform as a paradigm. The choice of Cd^(2+) as a probe was prompted by previous findings that xenobiotic metal ions modulate PKC......
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08-28-2021 07:56 AM
[NMR paper] NMR characterization of a multi-valent conjugate vaccine against Neisseria meningitidis A, C, W, Y and Haemophilus influenzae b infections
NMR characterization of a multi-valent conjugate vaccine against Neisseria meningitidis A, C, W, Y and Haemophilus influenzae b infections
Physicochemical technologies are a powerful tool for the structural characterization of vaccine antigens both at bulk level as well as on the final formulation. High-field Nuclear Magnetic Resonance (NMR) spectroscopy has been found to be an extremely and robust tool for tracking the industrial process manufacturing of carbohydrate-based vaccines. I have applied NMR spectroscopy to the characterization of a penta-valent conjugate vaccine against...
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08-14-2021 11:29 PM
[NMR paper] Quantification of residual cetyltrimethylammonium bromide (CTAB) and sodium deoxycholate (DOC) in Haemophilus influenzae type b (Hib) polysaccharide using NMR
Quantification of residual cetyltrimethylammonium bromide (CTAB) and sodium deoxycholate (DOC) in Haemophilus influenzae type b (Hib) polysaccharide using NMR
CTAB and DOC are used as reagents in the purification of Hib polysaccharide. Polysaccharide is purified by precipitation with CTAB from fermented broth followed by solvent extraction and DOC is used to remove the protein impurities. The reagents used in the purification process should be removed from the product as per regulatory requirements. These two residual reagents can be easily identified and quantified in purified Haemophilus...
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02-24-2021 05:50 AM
[NMR paper] Characterization of conjugation pattern in large polysaccharide-protein conjugates by NMR.
Characterization of conjugation pattern in large polysaccharide-protein conjugates by NMR.
Related Articles Characterization of conjugation pattern in large polysaccharide-protein conjugates by NMR.
Angew Chem Int Ed Engl. 2017 Oct 10;:
Authors: Giuntini S, Balducci E, Cerofolini L, Ravera E, Fragai M, Berti F, Luchinat C
Abstract
Carbohydrate-based vaccines are among the safest and most effective vaccines and represent potent tools for prevention of life-threatening bacterial infectious diseases, like meningitis and pneumonia....
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10-14-2017 02:04 AM
[NMR paper] Characterization of conjugation pattern in large polysaccharide-protein conjugates by NMR
Characterization of conjugation pattern in large polysaccharide-protein conjugates by NMR
Carbohydrate-based vaccines are among the safest and most effective vaccines and represent potent tools for prevention of life-threatening bacterial infectious diseases, like meningitis and pneumonia. The chemical conjugation of a weak antigen to protein as a source of T-cell epitopes generates a glycoconjugate vaccine, that results more immunogenic. Several methods have been used so far to characterize the resulting polysaccharide-protein conjugates. However, a reduced number of methodologies has...
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10-10-2017 09:37 PM
An Atypical ?/?-Hydrolase Fold Revealedin the Crystal Structure of Pimeloyl-Acyl Carrier Protein Methyl EsteraseBioG from Haemophilus influenzae
An Atypical ?/?-Hydrolase Fold Revealedin the Crystal Structure of Pimeloyl-Acyl Carrier Protein Methyl EsteraseBioG from Haemophilus influenzae
http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/bichaw/0/bichaw.ahead-of-print/acs.biochem.6b00818/20161121/images/medium/bi-2016-00818f_0010.gif
Biochemistry
DOI: 10.1021/acs.biochem.6b00818
http://feeds.feedburner.com/~ff/acs/bichaw?d=yIl2AUoC8zA
http://feeds.feedburner.com/~r/acs/bichaw/~4/CUC9biz-5ig
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11-21-2016 11:02 PM
[NMR paper] Evaluation of Michael-type Acceptor Reactivity of 5-Benzylidenebarbiturates, 5-benzylidenerhodanines, and Related Heterocycles Using NMR.
Evaluation of Michael-type Acceptor Reactivity of 5-Benzylidenebarbiturates, 5-benzylidenerhodanines, and Related Heterocycles Using NMR.
Related Articles Evaluation of Michael-type Acceptor Reactivity of 5-Benzylidenebarbiturates, 5-benzylidenerhodanines, and Related Heterocycles Using NMR.
Acta Chim Slov. 2014 Sep;61(3):637-644
Authors: Arsovska E, Trontelj J, Zidar N, Tomaši? T, Maši? LP, Kikelj D, Plavec J, Zega A
Abstract
Despite existing experimental and computational tools to assess the risk, the non-specific chemical...
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10-07-2014 02:31 PM
[NMR paper] NMR assignment of the hypothetical protein HI0004 from Haemophilus influenzae--a puta
NMR assignment of the hypothetical protein HI0004 from Haemophilus influenzae--a putative essential gene product.
Related Articles NMR assignment of the hypothetical protein HI0004 from Haemophilus influenzae--a putative essential gene product.
J Biomol NMR. 2004 May;29(1):101-2
Authors: Cheon Yeh D, Parsons JF, Parsons LM, Liu F, Eisenstein E, Orban J
Reactivity characterization of bromoacetyl derivatized polyribosylribitol polysaccharide in Haemophilus influenzae type b for PedvaxHIB by NMR spectroscopy
Linear Mode
