Fluorobenzene probes for protein profiling through selective cysteine labeling have been developed by rational reactivity tuning. Tuning was achieved by selecting an electron-withdrawing para-substituent combined with variation of the number of fluorine substituents. Optimized probes chemo-selectively arylated cysteine residues in proteins under aqueous conditions. Probes linked to azide, biotin or a fluorophore were applicable to labeling of eGFP and albumin. Selective inhibition of cysteine proteases was also demonstrated with the probes. Additionally, probes were tuned for site selective labeling among cysteine residues and for activity based protein profiling in cell lysates.
Tuning the Flexibility of Glycine-Serine Linkers ToAllow Rational Design of Multidomain Proteins
Tuning the Flexibility of Glycine-Serine Linkers ToAllow Rational Design of Multidomain Proteins
http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/bichaw/0/bichaw.ahead-of-print/acs.biochem.7b00902/20171207/images/medium/bi-2017-00902m_0006.gif
Biochemistry
DOI: 10.1021/acs.biochem.7b00902
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12-08-2017 01:06 AM
19F Dynamic Nuclear Polarization and SEM in Suspensions Consisting of Fluorobenzene Derivatives and Asphaltene Extracted from MC-800 Liquid Asphalt #DNPNMR
From The DNP-NMR Blog:
19F Dynamic Nuclear Polarization and SEM in Suspensions Consisting of Fluorobenzene Derivatives and Asphaltene Extracted from MC-800 Liquid Asphalt #DNPNMR
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Kirimli, H.E. and H. Ovalioglu, 19F Dynamic Nuclear Polarization and SEM in Suspensions Consisting of Fluorobenzene Derivatives and Asphaltene Extracted from MC-800 Liquid Asphalt. Journal of Dispersion Science and Technology, 2014. 35(2): p. 255-264.
https://doi.org/10.1080/01932691.2013.767208
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01-09-2017 04:21 PM
[NMR paper] Modification of Bafilomycin Structure to Efficiently Synthesize Solid-State NMR Probes that Selectively Bind to Vacuolar-Type ATPase.
Modification of Bafilomycin Structure to Efficiently Synthesize Solid-State NMR Probes that Selectively Bind to Vacuolar-Type ATPase.
Related Articles Modification of Bafilomycin Structure to Efficiently Synthesize Solid-State NMR Probes that Selectively Bind to Vacuolar-Type ATPase.
Chem Asian J. 2015 Jan 21;
Authors: Shibata H, Tsuchikawa H, Hayashi T, Matsumori N, Murata M, Usui T
Abstract
Bafilomycin (Baf) is one of the most potent inhibitors of vacuolar-type ATPase, which is strongly implicated in age-related diseases....
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01-22-2015 01:20 PM
Journal Highlight: On the tuning of high-resolution NMR probes
Journal Highlight: On the tuning of high-resolution NMR probes
http://www.spectroscopynow.com/common/images/thumbnails/14a0562f4b6.jpgThree optimum conditions for the tuning of NMR probes are compared: the conventional tuning optimum, the spin noise tuning optimum and the newly introduced frequency shift tuning optimum.
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General
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12-01-2014 12:17 PM
[NMR paper] 19F NMR of trifluoroacetyl-labeled cysteine mutants of myoglobin: structural probes o
19F NMR of trifluoroacetyl-labeled cysteine mutants of myoglobin: structural probes of nitric oxide bound to the H93G cavity mutant.
Related Articles 19F NMR of trifluoroacetyl-labeled cysteine mutants of myoglobin: structural probes of nitric oxide bound to the H93G cavity mutant.
Biochemistry. 2001 Jul 24;40(29):8588-96
Authors: Thomas MR, Boxer SG
Nitric oxide (NO) binds to the myoglobin (Mb) cavity mutant, H93G, forming either a 5- or 6-coordinate Fe--NO heme complex. The H93G mutation replaces the proximal histidine of Mb with glycine,...
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11-19-2010 08:44 PM
[NMR paper] Improved NMR spectra of a protein-DNA complex through rational mutagenesis and the ap
Improved NMR spectra of a protein-DNA complex through rational mutagenesis and the application of a sensitivity optimized isotope-filtered NOESY experiment.
Related Articles Improved NMR spectra of a protein-DNA complex through rational mutagenesis and the application of a sensitivity optimized isotope-filtered NOESY experiment.
J Biomol NMR. 2001 Mar;19(3):231-41
Authors: Iwahara J, Wojciak JM, Clubb RT
The NMR spectra of the complex between the DNA-binding domain of the Dead ringer protein (DRI-DBD, Gly262-Gly398) and its DNA binding site...
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11-19-2010 08:32 PM
Signal enhancement in protein NMR using the spin-noise tuning optimum.
Signal enhancement in protein NMR using the spin-noise tuning optimum.
Signal enhancement in protein NMR using the spin-noise tuning optimum.
J Biomol NMR. 2010 Oct 6;
Authors: Nausner M, Goger M, Bendet-Taicher E, Schlagnitweit J, Jerschow A, Müller N
We have assessed the potential of an alternative probe tuning strategy based on the spin-noise response for application in common high-resolution multi-dimensional biomolecular NMR experiments with water signal suppression on aqueous and salty samples. The method requires the adjustment of the...
Rational tuning of fluorobenzene probes for cysteine-selective protein modification
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