Due to their exceptional anisotropic magnetic properties, lanthanide ion (Ln^(3+)) complexes are of great utility in many fields of chemistry, including magnetic materials, biomedical imaging, and nuclear magnetic resonance (NMR) spectroscopy. How to achieve large magnetic anisotropies in the Ln^(3+) complexes coordinated with open-chain ligands is still a challenge. In this study, we started from the open-chain 4PS-PyMTA ligand and assessed the magnetic anisotropy using installed Ln^(3+)...
Conformationally locked lanthanide chelating tags for convenient pseudocontact shift protein nuclear magnetic resonance spectroscopy
Conformationally locked lanthanide chelating tags for convenient pseudocontact shift protein nuclear magnetic resonance spectroscopy
Abstract
Pseudocontact shifts (PCS) generated by lanthanide chelating tags yield valuable restraints for investigating protein structures, dynamics and interactions in solution. In this work, dysprosium-, thulium- and terbium-complexes of eight-fold methylated 1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetic acid tags R4S)-SSPy] are presented that induce large pseudocontact shifts up to 5.5Â*ppm and adopt...
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11-25-2018 06:02 AM
[NMR paper] Compact, hydrophilic, lanthanide-binding tags for paramagnetic NMR spectroscopy.
Compact, hydrophilic, lanthanide-binding tags for paramagnetic NMR spectroscopy.
Compact, hydrophilic, lanthanide-binding tags for paramagnetic NMR spectroscopy.
Chem Sci. 2015 Apr 01;6(4):2614-2624
Authors: Lee MD, Loh CT, Shin J, Chhabra S, Dennis ML, Otting G, Swarbrick JD, Graham B
Abstract
The design, synthesis and evaluation of four novel lanthanide-binding tags for paramagnetic NMR spectroscopy are reported. Each tag is based on the...
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03-23-2018 11:18 AM
[NMR paper] Short two-armed lanthanide-binding tags for paramagnetic NMR spectroscopy based on chiral 1,4,7,10-tetrakis(2-hydroxypropyl)-1,4,7,10-tetraazacyclododecane scaffolds.
Short two-armed lanthanide-binding tags for paramagnetic NMR spectroscopy based on chiral 1,4,7,10-tetrakis(2-hydroxypropyl)-1,4,7,10-tetraazacyclododecane scaffolds.
http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--www.rsc.org-images-entities-char_z_RSClogo.gif Short two-armed lanthanide-binding tags for paramagnetic NMR spectroscopy based on chiral 1,4,7,10-tetrakis(2-hydroxypropyl)-1,4,7,10-tetraazacyclododecane scaffolds.
Chem Commun (Camb). 2017 Nov 22;:
Authors: Lee MD, Dennis ML, Graham B, Swarbrick JD
...
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11-24-2017 04:57 AM
Conformational dynamics and alignment properties of loop lanthanide-binding-tags (LBTs) studied in interleukin-1β
Conformational dynamics and alignment properties of loop lanthanide-binding-tags (LBTs) studied in interleukin-1β
Abstract
Encodable lanthanide binding tags (LBTs) have become an attractive tool in modern structural biology as they can be expressed as fusion proteins of targets of choice. Previously, we have demonstrated the feasibility of inserting encodable LBTs into loop positions of interleukin-1β (Barthelmes et al. in J Am Chem Soc 133:808â??819, 2011). Here, we investigate the differences in fast dynamics of selected loop-LBT interleukin-1β...
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05-23-2017 04:44 AM
[NMR paper] Enantiomeric two-armed lanthanide-binding tags for complementary effects in paramagnetic NMR spectroscopy.
Enantiomeric two-armed lanthanide-binding tags for complementary effects in paramagnetic NMR spectroscopy.
Related Articles Enantiomeric two-armed lanthanide-binding tags for complementary effects in paramagnetic NMR spectroscopy.
Chem Commun (Camb). 2016 Jun 2;
Authors: Lee MD, Dennis ML, Swarbrick JD, Graham B
Abstract
Two-armed lanthanide-binding tags induce significant, long-range paramagnetic effects in the NMR spectra of attached proteins. An enantiomeric pair of rigid, two-armed, cyclen-based tags are reported that...
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06-03-2016 04:52 PM
Pulse EPR-enabled interpretation of scarce pseudocontact shifts induced by lanthanide binding tags
Pulse EPR-enabled interpretation of scarce pseudocontact shifts induced by lanthanide binding tags
Abstract
Pseudocontact shifts (PCS) induced by tags loaded with paramagnetic lanthanide ions provide powerful long-range structure information, provided the location of the metal ion relative to the target protein is known. Usually, the metal position is determined by fitting the magnetic susceptibility anisotropy (Î?Ï?) tensor to the 3D structure of the protein in an 8-parameter fit, which requires a large set of PCSs to be reliable. In an alternative...
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11-23-2015 06:58 PM
[NMR paper] Encoded loop-lanthanide-binding tags for long-range distance measurements in proteins by NMR and EPR spectroscopy.
Encoded loop-lanthanide-binding tags for long-range distance measurements in proteins by NMR and EPR spectroscopy.
Related Articles Encoded loop-lanthanide-binding tags for long-range distance measurements in proteins by NMR and EPR spectroscopy.
J Biomol NMR. 2015 Sep 4;
Authors: Barthelmes D, Gränz M, Barthelmes K, Allen KN, Imperiali B, Prisner T, Schwalbe H
Abstract
We recently engineered encodable lanthanide binding tags (LBTs) into proteins and demonstrated their applicability in Nuclear Magnetic Resonance (NMR)...
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09-06-2015 03:37 PM
Encoded loop-lanthanide-binding tags for long-range distance measurements in proteins by NMR and EPR spectroscopy
Encoded loop-lanthanide-binding tags for long-range distance measurements in proteins by NMR and EPR spectroscopy
Abstract
We recently engineered encodable lanthanide binding tags (LBTs) into proteins and demonstrated their applicability in Nuclear Magnetic Resonance (NMR) spectroscopy, X-ray crystallography and luminescence studies. Here, we engineered two-loop-LBTs into the model protein interleukin-1β (IL1β) and measured 1H, 15N-pseudocontact shifts (PCSs) by NMR spectroscopy. We determined the Î?Ï?-tensors associated with each Tm3+-loaded...
Rational Design of Lanthanide-Binding Tags to Optimize Magnetic Anisotropy in Paramagnetic Protein NMR
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