Elucidating the biochemical roles of the essential metal ion, Zn^(2+), motivates detection strategies that are sensitive, selective, quantitative, and minimally invasive in living systems. Fluorescent probes have identified Zn^(2+) in cells but complementary approaches employing nuclear magnetic resonance (NMR) are lacking. Recent studies of maltose binding protein (MBP) using ultrasensitive ^(129)Xe NMR spectroscopy identified a switchable salt bridge which causes slow xenon exchange and...
[NMR paper] Accessing isotopically labeled proteins containing genetically encoded phosphoserine for NMR with optimized expression conditions
Accessing isotopically labeled proteins containing genetically encoded phosphoserine for NMR with optimized expression conditions
Phosphoserine (pSer) sites are primarily located within disordered protein regions, making it difficult to experimentally ascertain their effects on protein structure and function. Therefore, the production of ^(15)N (and ^(13)C)-labeled proteins with site-specifically encoded pSer for Nuclear Magnetic Resonance (NMR) studies is essential to uncover molecular mechanisms of protein regulation by phosphorylation. While genetic code expansion (GCE) technologies for...
[NMR paper] A Genetically Encoded F-19 NMR Probe Reveals the Allosteric Modulation Mechanism of Cannabinoid Receptor 1
A Genetically Encoded F-19 NMR Probe Reveals the Allosteric Modulation Mechanism of Cannabinoid Receptor 1
Due to the lack of genetically encoded probes for fluorine-19 nuclear magnetic resonance spectroscopy (^(19)F NMR), its utility for probing eukaryotic membrane protein dynamics is limited. Here we report an efficient method for the genetic incorporation of an unnatural amino acid (UAA), 3'-trifluoromenthyl-phenylalanine (mtfF), into cannabinoid receptor 1 (CB1) in the Baculovirus Expression System. The probe can be inserted at any environmentally sensitive site, while causing minimal...
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[NMR paper] A genetically encoded 19F NMR probe for lysine acetylation.
A genetically encoded 19F NMR probe for lysine acetylation.
A genetically encoded 19F NMR probe for lysine acetylation.
Chem Commun (Camb). 2018 Mar 29;:
Authors: Zhang F, Zhou Q, Yang G, An L, Li F, Wang J
Abstract
Advances in acetylated protein-protein/DNA interactions depend on the development of a novel NMR (nuclear magnetic resonance) probe to study the conformational changes of acetylated proteins. However, the method for detecting the acetylated protein conformation is underdeveloped. Herein, an acetyllysine mimic has...
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03-30-2018 06:40 PM
[NMR paper] Nanomolar small-molecule detection using a genetically encoded 129Xe NMR contrast agent.
Nanomolar small-molecule detection using a genetically encoded 129Xe NMR contrast agent.
Nanomolar small-molecule detection using a genetically encoded 129Xe NMR contrast agent.
Chem Sci. 2017 Nov 01;8(11):7631-7636
Authors: Roose BW, Zemerov SD, Dmochowski IJ
Abstract
Genetically encoded magnetic resonance imaging (MRI) contrast agents enable non-invasive detection of specific biomarkers in vivo. Here, we employed the hyper-CEST 129Xe NMR technique to quantify maltose (32 nM to 1 mM) through its modulation of conformational...
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03-24-2018 12:21 PM
[NMR paper] A Genetically Encoded ?-Lactamase Reporter for Ultrasensitive (129) Xe NMR in Mammalian Cells.
A Genetically Encoded ?-Lactamase Reporter for Ultrasensitive (129) Xe NMR in Mammalian Cells.
Related Articles A Genetically Encoded ?-Lactamase Reporter for Ultrasensitive (129) Xe NMR in Mammalian Cells.
Angew Chem Int Ed Engl. 2016 Jun 15;
Authors: Wang Y, Roose BW, Palovcak EJ, Carnevale V, Dmochowski IJ
Abstract
Molecular imaging holds considerable promise for elucidating biological processes in normal physiology as well as disease states, but requires noninvasive methods for identifying analytes at sub-micromolar...
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06-16-2016 12:06 PM
[NMR paper] A Genetically Encoded 19 F NMR Probe for Tyrosine Phosphorylation.
A Genetically Encoded 19 F NMR Probe for Tyrosine Phosphorylation.
A Genetically Encoded 19 F NMR Probe for Tyrosine Phosphorylation.
Angew Chem Int Ed Engl. 2013 Feb 28;
Authors: Li F, Shi P, Li J, Yang F, Wang T, Zhang W, Gao F, Ding W, Li D, Li J, Xiong Y, Sun J, Gong W, Tian C, Wang J
Abstract
Simple and selective: Tyrosine phosphorylation is a pivotal post-translational modification which regulates the enzymatic activity, protein conformation, and protein-protein interactions. The highly efficient genetic incorporation of...
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03-02-2013 11:45 AM
Generation of Pseudocontact Shifts in Protein NMR Spectra with a Genetically Encoded
Generation of Pseudocontact Shifts in Protein NMR Spectra with a Genetically Encoded Cobalt(II)-Binding Amino Acid.
Related Articles Generation of Pseudocontact Shifts in Protein NMR Spectra with a Genetically Encoded Cobalt(II)-Binding Amino Acid.
Angew Chem Int Ed Engl. 2010 Nov 25;
Authors: Nguyen TH, Ozawa K, Stanton-Cook M, Barrow R, Huber T, Otting G
Rational design of a genetically encoded NMR zinc sensor
Linear Mode
