Rapid Proton-DetectedNMR Assignment for Proteinswith Fast Magic Angle Spinning
Rapid Proton-DetectedNMR Assignment for Proteinswith Fast Magic Angle Spinning
Emeline Barbet-Massin, Andrew J. Pell, Joren S. Retel, Loren B. Andreas, Kristaps Jaudzems, W. Trent Franks, Andrew J. Nieuwkoop, Matthias Hiller, Victoria Higman, Paul Guerry, Andrea Bertarello, Michael J. Knight, Michele Felletti, Tanguy Le Marchand, Svetlana Kotelovica, Inara Akopjana, Kaspars Tars, Monica Stoppini, Vittorio Bellotti, Martino Bolognesi, Stefano Ricagno, James J. Chou, Robert G. Griffin, Hartmut Oschkinat, Anne Lesage, Lyndon Emsley, Torsten Herrmann and Guido Pintacuda
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08-18-2014 10:14 PM
Interactions of protein side chains with RNA defined with REDOR solid state NMR.
Interactions of protein side chains with RNA defined with REDOR solid state NMR.
Interactions of protein side chains with RNA defined with REDOR solid state NMR.
J Biomol NMR. 2011 Sep 25;
Authors: Huang W, Varani G, Drobny GP
Abstract
Formation of the complex between human immunodeficiency virus type-1 Tat protein and the transactivation response region (TAR) RNA is vital for transcriptional elongation, yet the structure of the Tat-TAR complex remains to be established. The NMR structures of free TAR, and TAR bound to Tat-derived...
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09-30-2011 06:00 AM
Interactions of protein side chains with RNA defined with REDOR solid state NMR.
Interactions of protein side chains with RNA defined with REDOR solid state NMR.
Interactions of protein side chains with RNA defined with REDOR solid state NMR.
J Biomol NMR. 2011 Sep 25;
Authors: Huang W, Varani G, Drobny GP
Abstract
Formation of the complex between human immunodeficiency virus type-1 Tat protein and the transactivation response region (TAR) RNA is vital for transcriptional elongation, yet the structure of the Tat-TAR complex remains to be established. The NMR structures of free TAR, and TAR bound to Tat-derived...
nmrlearner
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0
09-30-2011 05:59 AM
Interactions of protein side chains with RNA defined with REDOR solid state NMR
Interactions of protein side chains with RNA defined with REDOR solid state NMR
Abstract Formation of the complex between human immunodeficiency virus type-1 Tat protein and the transactivation response region (TAR) RNA is vital for transcriptional elongation, yet the structure of the Tat-TAR complex remains to be established. The NMR structures of free TAR, and TAR bound to Tat-derived peptides have been obtained by solution NMR, but only a small number of intermolecular NOEs could be identified unambiguously, preventing the determination of a complete structure. Here we show that a...
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09-27-2011 07:04 AM
[NMR paper] ALARM NMR: a rapid and robust experimental method to detect reactive false positives
ALARM NMR: a rapid and robust experimental method to detect reactive false positives in biochemical screens.
Related Articles ALARM NMR: a rapid and robust experimental method to detect reactive false positives in biochemical screens.
J Am Chem Soc. 2005 Jan 12;127(1):217-24
Authors: Huth JR, Mendoza R, Olejniczak ET, Johnson RW, Cothron DA, Liu Y, Lerner CG, Chen J, Hajduk PJ
High-throughput screening (HTS) of large compound collections typically results in numerous small molecule hits that must be carefully evaluated to identify valid drug...
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11-24-2010 11:14 PM
[NMR paper] A general NMR method for rapid, efficient, and reliable biochemical screening.
A general NMR method for rapid, efficient, and reliable biochemical screening.
Related Articles A general NMR method for rapid, efficient, and reliable biochemical screening.
J Am Chem Soc. 2003 Nov 26;125(47):14620-5
Authors: Dalvit C, Ardini E, Flocco M, Fogliatto GP, Mongelli N, Veronesi M
High-throughput screening is usually the method of drug-lead discovery. It is now well accepted that, for a functional assay, quality is more important than quantity. The ligand-based or protein-based NMR screening methodologies for detecting compounds...
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11-24-2010 09:16 PM
[NMR paper] Microdrop screening: a rapid method to optimize solvent conditions for NMR spectrosco
Microdrop screening: a rapid method to optimize solvent conditions for NMR spectroscopy of proteins.
Related Articles Microdrop screening: a rapid method to optimize solvent conditions for NMR spectroscopy of proteins.
J Biomol NMR. 1998 Nov;12(4):493-9
Authors: Lepre CA, Moore JM
Determining appropriate solvent conditions is a crucial first step for carrying out NMR spectroscopy of proteins, but rapid and efficient methods for doing so are currently lacking. Microdrop screening examines a large number of different solvent conditions using...
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11-17-2010 11:15 PM
[NMR paper] Rapid and simple approach for the NMR resonance assignment of the carbohydrate chains
Rapid and simple approach for the NMR resonance assignment of the carbohydrate chains of an intact glycoprotein. Application of gradient-enhanced natural abundance 1H-13C HSQC and HSQC-TOCSY to the alpha-subunit of human chorionic gonadotropin.
http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--linkinghub.elsevier.com-ihub-images-PubMedLink.gif Related Articles Rapid and simple approach for the NMR resonance assignment of the carbohydrate chains of an intact glycoprotein. Application of gradient-enhanced natural abundance 1H-13C HSQC and HSQC-TOCSY to the alpha-subunit of...
A Rapid and Versatile Method for Generating Proteinswith Defined Ubiquitin Chains
Linear Mode
