[NMR paper] Radio frequency gradient enhanced diffusion-edited semi-solid state NMR spectroscopy for detailed structural characterization of chemically modified hyaluronic acid hydrogels
Radio frequency gradient enhanced diffusion-edited semi-solid state NMR spectroscopy for detailed structural characterization of chemically modified hyaluronic acid hydrogels
Applications of functionalized hyaluronic acid (HA) hydrogels for numerous biomedical applications requires their detailed structural characterization. Since these materials are prepared by multistep chemical modifications in the solid phase and not amenable to characterization by standard analytical tools, we employed high-resolution solid-state NMR spectroscopy to gain detailed insights into the structures of the functionalized HA hydrogels. Divinyl sulfone crosslinked HA hydrogels were...
[NMR paper] Resolving Atomic-Level Dynamics and Interactions of High-Molecular-Weight Hyaluronic Acid by Multidimensional Solid-State NMR
Resolving Atomic-Level Dynamics and Interactions of High-Molecular-Weight Hyaluronic Acid by Multidimensional Solid-State NMR
High-molecular-weight (HMW) hyaluronic acid (HA) is a highly abundant natural polysaccharide and a fundamental component of the extracellular matrix (ECM). Its size and concentration regulate tissues' macro- and microenvironments, and its upregulation is a hallmark feature of certain tumors. Yet, the conformational dynamics of HMW-HA and how it engages with the components of the ECM microenvironment remain poorly understood at the molecular level. Probing the...
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[NMR paper] Production of isotopically enriched high molecular weight hyaluronic acid and characterization by solid-state NMR
Production of isotopically enriched high molecular weight hyaluronic acid and characterization by solid-state NMR
Hyaluronic acid (HA) is a naturally occurring polysaccharide that is abundant in the extracellular matrix (ECM) of all vertebrate cells. HA-based hydrogels have attracted great interest for biomedical applications due to their high viscoelasticity and biocompatibility. In both ECM and hydrogel applications, high molecular weight (HMW)-HA can absorb a large amount of water to yield matrices with a high level of structural integrity. To understand the molecular underpinnings of...
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[NMR paper] Protein structural changes characterized by high-pressure, pulsed field gradient diffusion NMR spectroscopy.
Protein structural changes characterized by high-pressure, pulsed field gradient diffusion NMR spectroscopy.
http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/https:--linkinghub.elsevier.com-ihub-images-PubMedLink.gif Related Articles Protein structural changes characterized by high-pressure, pulsed field gradient diffusion NMR spectroscopy.
J Magn Reson. 2020 Feb 19;312:106701
Authors: Ramanujam V, Alderson TR, Pritišanac I, Ying J, Bax A
Abstract
Pulsed-field gradient NMR spectroscopy is widely used to measure...
[NMR paper] A unified heteronuclear decoupling picture in solid-state NMR under low radio-frequency amplitude and fast magic-angle-spinning frequency regime.
A unified heteronuclear decoupling picture in solid-state NMR under low radio-frequency amplitude and fast magic-angle-spinning frequency regime.
http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/https:--publishing.aip.org-sites-default-files-aippub-NLM-scitationblue.jpg Related Articles A unified heteronuclear decoupling picture in solid-state NMR under low radio-frequency amplitude and fast magic-angle-spinning frequency regime.
J Chem Phys. 2019 Apr 14;150(14):144201
Authors: Sharma K, Equbal A, Nielsen NC, Madhu PK
...
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[NMR paper] Direct Evidence of Imino Acid-Aromatic Interactions in Native Collagen Protein by DNP-Enhanced Solid-State NMR Spectroscopy.
Direct Evidence of Imino Acid-Aromatic Interactions in Native Collagen Protein by DNP-Enhanced Solid-State NMR Spectroscopy.
Related Articles Direct Evidence of Imino Acid-Aromatic Interactions in Native Collagen Protein by DNP-Enhanced Solid-State NMR Spectroscopy.
J Phys Chem Lett. 2014 Nov 20;5(22):4044-8
Authors: Singh C, Rai RK, Aussenac F, Sinha N
Abstract
Aromatic amino acids (AAAs) have rare presence (~1.4% abundance of Phe) inside of collagen protein, which is the most abundant animal protein playing a functional role in...
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08-16-2015 07:01 PM
[NMR paper] Proton-detected 2D radio frequency driven recoupling solid-state NMR studies on micelle-associated cytochrome-b5.
Proton-detected 2D radio frequency driven recoupling solid-state NMR studies on micelle-associated cytochrome-b5.
Related Articles Proton-detected 2D radio frequency driven recoupling solid-state NMR studies on micelle-associated cytochrome-b5.
J Magn Reson. 2014 Mar 1;242C:169-179
Authors: Pandey MK, Vivekanandan S, Yamamoto K, Im S, Waskell L, Ramamoorthy A
Abstract
Solid-state NMR spectroscopy is increasingly used in the high-resolution structural studies of membrane-associated proteins and peptides. Most such studies necessitate...
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03-25-2014 11:49 AM
[NMR paper] Proton-Detected 2D Radio Frequency Driven Recoupling Solid-state NMR Studies on Micelle-associated Cytochrome-b5
Proton-Detected 2D Radio Frequency Driven Recoupling Solid-state NMR Studies on Micelle-associated Cytochrome-b5
Publication date: Available online 1 March 2014
Source:Journal of Magnetic Resonance</br>
Author(s): Manoj Kumar Pandey , Subramanian Vivekanandan , Kazutoshi Yamamoto , Sangchoul Im , Lucy Waskell , Ayyalusamy Ramamoorthy</br>
Solid-state NMR spectroscopy is increasingly used in the high-resolution structural studies of membrane-associated proteins and peptides. Most such studies necessitate isotopically labeled (13C, 15N and 2H) proteins/peptides,...
Radio frequency gradient enhanced diffusion-edited semi-solid state NMR spectroscopy for detailed structural characterization of chemically modified hyaluronic acid hydrogels
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