Quick, sensitive serial NMR experiments with Radon transform.

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Quick, sensitive serial NMR experiments with Radon transform.

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08-02-2017, 01:00 AM

nmrlearner

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Quick, sensitive serial NMR experiments with Radon transform.

Quick, sensitive serial NMR experiments with Radon transform.

Publication date: Available online 31 July 2017
Source:Journal of Magnetic Resonance

Author(s): Rupashree Dass, Pawe? Kasprzak, Krzysztof Kazimierczuk

The Radon transform is a potentially powerful tool for processing the data from serial spectroscopic experiments. It makes it possible to decode the rate at which frequencies of spectral peaks shift under the effect of changing conditions, such as temperature, pH, or solvent. In this paper we show how it also improves speed and sensitivity, especially in multidimensional experiments. This is particularly important in the case of low-sensitivity techniques, such as NMR spectroscopy. As an example, we demonstrate how Radon transform processing allows serial measurements of 15 N -HSQC spectra of unlabelled peptides that would otherwise be infeasible.
Graphical abstract

Source: Journal of Magnetic Resonance

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