NMR paper Quantitative NMR for the structural analysis of novel bivalent glycoconjugates as vaccine candidates

		BioNMR > Educational resources > Journal club
[NMR paper] Quantitative NMR for the structural analysis of novel bivalent glycoconjugates as vaccine candidates

Webservers

NMR processing:

MDD

NMR assignment:

Backbone:

Side-chains:

NOEs:

Structure from NMR restraints:

Ab initio:

Fragment-based:

Rosetta-NMR (Robetta)

Template-based:

GeNMR

I-TASSER

Refinement:

Amber

Structure from chemical shifts:

Fragment-based:

Homology-based:

Torsion angles from chemical shifts:

Preditor

TALOS

Promega- Proline

Secondary structure from chemical shifts:

CSI (via RCI server)

TALOS

MICS caps, β-turns

d2D

PECAN

Flexibility from chemical shifts:

RCI

Interactions from chemical shifts:

HADDOCK

Chemical shifts re-referencing:

NMR model quality:

NOEs, other restraints:

Chemical shifts:

RDCs:

Pseudocontact shifts:

Protein geomtery:

SAVES2 or SAVES4

Quality Control Check

NMR spectrum prediction:

FANDAS

MestReS

V-NMR

Flexibility from structure:

Backbone S2

Methyl S2

B-factor

Molecular dynamics:

Gromacs

Amber

Antechamber

Chemical shifts prediction:

From structure:

Shiftx2

Sparta+

Camshift

CH3shift- Methyl

ArShift- Aromatic

ShiftS

Proshift

PPM

CheShift-2- Cα

From sequence:

Shifty

Camcoil

Poulsen_rc_CS

Disordered proteins:

MAXOCC

Format conversion & validation:

CCPN

From NMR-STAR 3.1

Validate NMR-STAR 3.1

NMR sample preparation:

Protein disorder:

DisMeta

Protein solubility:

Isotope labeling:

Solid-state NMR:

Thread Tools

Search this Thread

Rate Thread

Display Modes

03-27-2022, 12:20 PM

nmrlearner

Senior Member

Join Date: Jan 2005

Posts: 23,732

Points: 193,617, Level: 100

Level up: 0%, 0 Points needed

Activity: 50.7%

Last Achievements

Award-Showcase

NMR Credits: 0

NMR Points: 193,617

Downloads: 0

Uploads: 0

Quantitative NMR for the structural analysis of novel bivalent glycoconjugates as vaccine candidates

Quantitative NMR for the structural analysis of novel bivalent glycoconjugates as vaccine candidates

Novel unimolecular bivalent glycoconjugates were assembled combining several functionalized capsular polysaccharides of Streptococcus pneumoniae and Neisseria meningitidis to a carrier protein by using an effective strategy based on the Ugi 4-component reaction. The development of multivalent glycoconjugates opens new opportunities in the field of vaccine design, but their high structural complexity involves new analytical challenges. Nuclear Magnetic Resonance has found wide applications in the...

More...

Did you find this post helpful?

Similar Threads
Thread	Thread Starter	Forum	Replies	Last Post
Kawasaki disease: Protein biomarker candidates - Ezine - spectroscopyNOW.com Kawasaki disease: Protein biomarker candidates - Ezine - spectroscopyNOW.com Kawasaki disease: Protein biomarker candidates - Ezine spectroscopyNOW.comKawasaki disease is a rare condition that affects children under the age of five. Also known as mucocutaneous lymph node syndrome, it is rarely fatal but can ... Read here	nmrlearner	Online News	0	03-30-2019 05:11 AM
Kawasaki disease: Protein biomarker candidates - Ezine - spectroscopyNOW.com Kawasaki disease: Protein biomarker candidates - Ezine - spectroscopyNOW.com Kawasaki disease: Protein biomarker candidates - Ezine spectroscopyNOW.comKawasaki disease is a rare condition that affects children under the age of five. Also known as mucocutaneous lymph node syndrome, it is rarely fatal but can ... Read here	nmrlearner	Online News	0	03-24-2019 10:41 PM
[ASAP] Quantitative Characterization of Bivalent Probes for a Dual Bromodomain Protein, Transcription Initiation Factor TFIID Subunit 1 Quantitative Characterization of Bivalent Probes for a Dual Bromodomain Protein, Transcription Initiation Factor TFIID Subunit 1 https://pubs.acs.org/appl/literatum/publisher/achs/journals/content/bichaw/0/bichaw.ahead-of-print/acs.biochem.8b00150/20180328/images/medium/bi-2018-00150k_0006.gif Biochemistry DOI: 10.1021/acs.biochem.8b00150 http://feeds.feedburner.com/~ff/acs/bichaw?d=yIl2AUoC8zA http://feeds.feedburner.com/~r/acs/bichaw/~4/wc2_SENodAA More...	nmrlearner	Journal club	0	03-29-2018 05:39 AM
Quantitative analysis of protein–ligand interactions by NMR Quantitative analysis of protein–ligand interactions by NMR Publication date: Available online 3 March 2016 Source:Progress in Nuclear Magnetic Resonance Spectroscopy</br> Author(s): Ayako Furukawa, Tsuyoshi Konuma, Saeko Yanaka, Kenji Sugase</br> Protein–ligand interactions have been commonly studied through static structures of the protein–ligand complex. Recently, however, there has been increasing interest in investigating the dynamics of protein–ligand interactions both for fundamental understanding of the underlying mechanisms and for drug development....	nmrlearner	Journal club	0	03-03-2016 08:32 PM
ASU center seeks potential vaccine for tularemia - Vaccine News Daily http://www.bionmr.com//t3.gstatic.com/images?q=tbn:ANd9GcQ6qKuM7cxI6KDroGjcLdFAIb5kokLOd6-Ve2yUtfausAAPL6vw90fIm0s8-2aY8hSYZvCnQxil Vaccine News Daily <img alt="" height="1" width="1"> ASU center seeks potential vaccine for tularemia Vaccine News Daily Fromme and her ASU colleagues used nuclear magnetic resonance (NMR) spectroscopy to explore detailed features of the bacteria's surface protein. Their research marks the first structural determination at atomic scale for any lipoprotein of the genus ... ASU center seeks potential vaccine for tularemia - Vaccine News Daily	nmrlearner	Online News	0	06-23-2015 11:58 PM
[NMR paper] Structural analysis of oligosaccharides and glycoconjugates using NMR. Structural analysis of oligosaccharides and glycoconjugates using NMR. Related Articles Structural analysis of oligosaccharides and glycoconjugates using NMR. Adv Neurobiol. 2014;9:165-83 Authors: Yamaguchi Y, Yamaguchi T, Kato K Abstract Carbohydrate chains play critical roles in cellular recognition and subsequent signal transduction in the nervous system. Furthermore, gangliosides are targets for various amyloidogenic proteins associated with neurodegenerative disorders. To better understand the molecular mechanisms underlying...	nmrlearner	Journal club	0	08-26-2014 01:25 PM
[NMR paper] Structural characterization by NMR of a double phosphorylated chimeric Peptide vaccine for treatment of Alzheimer's disease. Structural characterization by NMR of a double phosphorylated chimeric Peptide vaccine for treatment of Alzheimer's disease. Structural characterization by NMR of a double phosphorylated chimeric Peptide vaccine for treatment of Alzheimer's disease. Molecules. 2013;18(5):4929-41 Authors: Ramírez-Gualito K, Richter M, Matzapetakis M, Singer D, Berger S Abstract Rational design of peptide vaccines becomes important for the treatment of some diseases such as Alzheimer's disease (AD) and related disorders. In this study, as part of a larger...	nmrlearner	Journal club	0	04-30-2013 10:21 PM
Journal Highlight: Quantitative NMR: An applicable method for quantitative analysis of medicinal plant extracts and herbal products Journal Highlight: Quantitative NMR: An applicable method for quantitative analysis of medicinal plant extracts and herbal products http://www.spectroscopynow.com/common/images/thumbnails/13abcca009b.jpgA quantitative NMR method has been reported for quantitative analysis of three medicinal plant extracts and their herbal products without the need of authentic standards. Source: Spectroscopynow.com	nmrlearner	General	0	02-03-2013 08:49 AM

« Previous Thread | Next Thread »

Posting Rules
You may not post new threads You may not post replies You may not post attachments You may not edit your posts BB code is On Smilies are On [IMG] code is On HTML code is On Trackbacks are Off Pingbacks are Off Refbacks are Off