NMR paper Quantitative NMR analysis of the kinetics of prenucleation oligomerization and aggregation of pathogenic huntingtin exon-1 protein

		BioNMR > Educational resources > Journal club
[NMR paper] Quantitative NMR analysis of the kinetics of prenucleation oligomerization and aggregation of pathogenic huntingtin exon-1 protein

Webservers

NMR processing:

MDD

NMR assignment:

Backbone:

Side-chains:

NOEs:

Structure from NMR restraints:

Ab initio:

Fragment-based:

Rosetta-NMR (Robetta)

Template-based:

GeNMR

I-TASSER

Refinement:

Amber

Structure from chemical shifts:

Fragment-based:

Homology-based:

Torsion angles from chemical shifts:

Preditor

TALOS

Promega- Proline

Secondary structure from chemical shifts:

CSI (via RCI server)

TALOS

MICS caps, β-turns

d2D

PECAN

Flexibility from chemical shifts:

RCI

Interactions from chemical shifts:

HADDOCK

Chemical shifts re-referencing:

NMR model quality:

NOEs, other restraints:

Chemical shifts:

RDCs:

Pseudocontact shifts:

Protein geomtery:

SAVES2 or SAVES4

Quality Control Check

NMR spectrum prediction:

FANDAS

MestReS

V-NMR

Flexibility from structure:

Backbone S2

Methyl S2

B-factor

Molecular dynamics:

Gromacs

Amber

Antechamber

Chemical shifts prediction:

From structure:

Shiftx2

Sparta+

Camshift

CH3shift- Methyl

ArShift- Aromatic

ShiftS

Proshift

PPM

CheShift-2- Cα

From sequence:

Shifty

Camcoil

Poulsen_rc_CS

Disordered proteins:

MAXOCC

Format conversion & validation:

CCPN

From NMR-STAR 3.1

Validate NMR-STAR 3.1

NMR sample preparation:

Protein disorder:

DisMeta

Protein solubility:

Isotope labeling:

Solid-state NMR:

Thread Tools

Search this Thread

Rate Thread

Display Modes

07-21-2022, 04:02 AM

nmrlearner

Senior Member

Join Date: Jan 2005

Posts: 23,732

Points: 193,617, Level: 100

Level up: 0%, 0 Points needed

Activity: 50.7%

Last Achievements

Award-Showcase

NMR Credits: 0

NMR Points: 193,617

Downloads: 0

Uploads: 0

Quantitative NMR analysis of the kinetics of prenucleation oligomerization and aggregation of pathogenic huntingtin exon-1 protein

Quantitative NMR analysis of the kinetics of prenucleation oligomerization and aggregation of pathogenic huntingtin exon-1 protein

The N-terminal region of the huntingtin protein, encoded by exon-1 (htt^(ex1)) and containing an expanded polyglutamine tract, forms fibrils that accumulate in neuronal inclusion bodies, resulting in Huntington's disease. We previously showed that reversible formation of a sparsely populated tetramer of the N-terminal amphiphilic domain, comprising a dimer of dimers in a four-helix bundle configuration, occurs on the microsecond timescale and is an essential prerequisite for subsequent...

More...

Did you find this post helpful?

Similar Threads
Thread	Thread Starter	Forum	Replies	Last Post
Quantitative NMR analysis of the kinetics of prenucleation oligomerization and aggregation of pathogenic huntingtin exon-1 protein \| Proceedings of the National Academy of Sciences - pnas.org Quantitative NMR analysis of the kinetics of prenucleation oligomerization and aggregation of pathogenic huntingtin exon-1 protein \| Proceedings of the National Academy of Sciences - pnas.org Quantitative NMR analysis of the kinetics of prenucleation oligomerization and aggregation of pathogenic huntingtin exon-1 protein \| Proceedings of the National Academy of Sciences pnas.org Read here	nmrlearner	Online News	0	07-21-2022 04:02 AM
Quantitative NMR analysis of the kinetics of prenucleation oligomerization and aggregation of pathogenic huntingtin exon-1 protein \| Proceedings of the National Academy of Sciences - pnas.org Quantitative NMR analysis of the kinetics of prenucleation oligomerization and aggregation of pathogenic huntingtin exon-1 protein \| Proceedings of the National Academy of Sciences - pnas.org Quantitative NMR analysis of the kinetics of prenucleation oligomerization and aggregation of pathogenic huntingtin exon-1 protein \| Proceedings of the National Academy of Sciences pnas.org Read here	nmrlearner	Online News	0	07-19-2022 05:10 PM
[NMR paper] Robust Cell-Free Expression of Sub-Pathological and Pathological Huntingtin Exon-1 for NMR Studies. General Approaches for the Isotopic Labeling of Low-Complexity Proteins. Robust Cell-Free Expression of Sub-Pathological and Pathological Huntingtin Exon-1 for NMR Studies. General Approaches for the Isotopic Labeling of Low-Complexity Proteins. Robust Cell-Free Expression of Sub-Pathological and Pathological Huntingtin Exon-1 for NMR Studies. General Approaches for the Isotopic Labeling of Low-Complexity Proteins. Biomolecules. 2020 Oct 19;10(10): Authors: Morató A, Elena-Real CA, Popovic M, Fournet A, Zhang K, Allemand F, Sibille N, Urbanek A, Bernadó P Abstract The high-resolution structural...	nmrlearner	Journal club	0	10-24-2020 10:43 PM
[ASAP] Interaction of Huntingtin Exon-1 Peptides with Lipid-Based Micellar Nanoparticles Probed by Solution NMR and Q-Band Pulsed EPR Interaction of Huntingtin Exon-1 Peptides with Lipid-Based Micellar Nanoparticles Probed by Solution NMR and Q-Band Pulsed EPR Alberto Ceccon, Thomas Schmidt, Vitali Tugarinov, Samuel A. Kotler, Charles D. Schwieters, G. Marius Clore https://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jacsat/0/jacsat.ahead-of-print/jacs.8b02619/20180514/images/medium/ja-2018-026194_0005.gif Journal of the American Chemical Society DOI: 10.1021/jacs.8b02619 http://feeds.feedburner.com/~ff/acs/jacsat?d=yIl2AUoC8zA http://feeds.feedburner.com/~r/acs/jacsat/~4/NazZqWqOVC4	nmrlearner	Journal club	0	05-15-2018 03:17 AM
Disordered Nanostructure in Huntingtin InteractingProtein K Acts as a Stabilizing Switch To Prevent Protein Aggregation Disordered Nanostructure in Huntingtin InteractingProtein K Acts as a Stabilizing Switch To Prevent Protein Aggregation http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/bichaw/0/bichaw.ahead-of-print/acs.biochem.7b00776/20180213/images/medium/bi-2017-00776d_0008.gif Biochemistry DOI: 10.1021/acs.biochem.7b00776 http://feeds.feedburner.com/~ff/acs/bichaw?d=yIl2AUoC8zA http://feeds.feedburner.com/~r/acs/bichaw/~4/943oCxcRWu4 More...	nmrlearner	Journal club	0	02-14-2018 02:43 AM
[NMR paper] Structure and Dynamics of the Huntingtin Exon-1 N-Terminus: ASolution NMR Perspective. Structure and Dynamics of the Huntingtin Exon-1 N-Terminus: ASolution NMR Perspective. Related Articles Structure and Dynamics of the Huntingtin Exon-1 N-Terminus: A*Solution NMR Perspective. J Am Chem Soc. 2017 Jan 13;: Authors: Baias M, Smith PE, Shen K, Joachimiak LA, ?erko S, Ko?mi?ski W, Frydman J, Frydman L Abstract	nmrlearner	Journal club	0	01-15-2017 02:57 AM
Structureand Dynamics of the Huntingtin Exon-1N-Terminus: ASolution NMR Perspective Structureand Dynamics of the Huntingtin Exon-1N-Terminus: ASolution NMR Perspective Maria Baias, Pieter E. S. Smith, Koning Shen, Lukasz A. Joachimiak, Szymon Z?erko, Wiktor Koz?min?ski, Judith Frydman and Lucio Frydman http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jacsat/0/jacsat.ahead-of-print/jacs.6b10893/20170113/images/medium/ja-2016-108934_0011.gif Journal of the American Chemical Society DOI: 10.1021/jacs.6b10893 http://feeds.feedburner.com/~ff/acs/jacsat?d=yIl2AUoC8zA http://feeds.feedburner.com/~r/acs/jacsat/~4/6vVAqMsGYCM	nmrlearner	Journal club	0	01-14-2017 06:24 AM
Difference between Extra- and IntracellularT1Values of Carboxylic Acids Affects the Quantitative Analysis of Cellular Kinetics by Hyperpolarized NMR From The DNP-NMR Blog: Difference between Extra- and IntracellularT1Values of Carboxylic Acids Affects the Quantitative Analysis of Cellular Kinetics by Hyperpolarized NMR p.p1 {margin: 0.0px 0.0px 0.0px 36.0px; text-indent: -36.0px; font: 12.0px Helvetica} Karlsson, M., et al., Difference between Extra- and IntracellularT1Values of Carboxylic Acids Affects the Quantitative Analysis of Cellular Kinetics by Hyperpolarized NMR. Angewandte Chemie, 2016. 128(43): p. 13765-13768. http://dx.doi.org/10.1002/ange.201607535	nmrlearner	News from NMR blogs	0	11-19-2016 08:35 PM

« Previous Thread | Next Thread »

Posting Rules
You may not post new threads You may not post replies You may not post attachments You may not edit your posts BB code is On Smilies are On [IMG] code is On HTML code is On Trackbacks are Off Pingbacks are Off Refbacks are Off