Solute translocation by membrane transport proteins is a vital biological process that can be tracked, on the sub-second timescale, using nuclear magnetic resonance (NMR). Fluorinated substrate analogues facilitate such studies because of high sensitivity of 19F NMR and absence of background signals. Accurate extraction of translocation rate constants requires precise quantification of NMR signal intensities. This becomes complicated in the presence of J-couplings, cross-correlations, and...
[NMR paper] Application of Dirichlet process mixture model to the identification of spin systems in protein NMR spectra.
Application of Dirichlet process mixture model to the identification of spin systems in protein NMR spectra.
Related Articles Application of Dirichlet process mixture model to the identification of spin systems in protein NMR spectra.
J Biomol NMR. 2018 May 18;:
Authors: Klukowski P, Augoff M, Zamorski M, Gonczarek A, Walczak MJ
Abstract
Analysis of structure, function and interactions of proteins by NMR spectroscopy usually requires the assignment of resonances to the corresponding nuclei in protein. This task, although...
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05-20-2018 06:02 PM
Application of Dirichlet process mixture model to the identification of spin systems in protein NMR spectra
Application of Dirichlet process mixture model to the identification of spin systems in protein NMR spectra
Abstract
Analysis of structure, function and interactions of proteins by NMR spectroscopy usually requires the assignment of resonances to the corresponding nuclei in protein. This task, although automated by methods such as FLYA or PINE, is still frequently performed manually. To facilitate the manual sequence-specific chemical shift assignment of complex proteins, we propose a method based on Dirichlet process mixture model (DPMM) that...
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05-19-2018 05:30 AM
Dynamic UltraFast 2D EXchange SpectroscopY (UF-EXSY) of hyperpolarized substrates
From The DNP-NMR Blog:
Dynamic UltraFast 2D EXchange SpectroscopY (UF-EXSY) of hyperpolarized substrates
Leon Swisher, C., et al., Dynamic UltraFast 2D EXchange SpectroscopY (UF-EXSY) of hyperpolarized substrates. J Magn Reson, 2015. 257: p. 102-9.
http://www.ncbi.nlm.nih.gov/pubmed/26117655
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09-10-2015 01:10 AM
[NMR paper] H/D exchange of a 15N labelled Tau fragment as measured by a simple Relax-EXSY experiment
H/D exchange of a 15N labelled Tau fragment as measured by a simple Relax-EXSY experiment
Publication date: December 2014
Source:Journal of Magnetic Resonance, Volume 249</br>
Author(s): Juan Lopez , Puneet Ahuja , Isabelle Landrieu , François-Xavier Cantrelle , Isabelle Huvent , Guy Lippens</br>
We present an equilibrium H/D exchange experiment to measure the exchange rates of labile amide protons in intrinsically unfolded proteins. By measuring the contribution of the H/D exchange to the apparent T 1 relaxation rates in solvents of different D2O content, we...
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11-14-2014 08:33 AM
[NMR paper] Quantitative Analysis of STD-NMR Spectra of Reversibly Forming Ligand-Receptor Complexes.
Quantitative Analysis of STD-NMR Spectra of Reversibly Forming Ligand-Receptor Complexes.
Related Articles Quantitative Analysis of STD-NMR Spectra of Reversibly Forming Ligand-Receptor Complexes.
Top Curr Chem. 2008;273:15-54
Authors: Krishna NR, Jayalakshmi V
Abstract
We describe our work on the quantitative analysis of STD-NMR spectra of reversibly forming ligand-receptorcomplexes. This analysis is based on the theory of complete relaxation and conformational exchange matrixanalysis of saturation transfer (CORCEMA-ST) effects. As part...
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04-24-2013 09:48 PM
Fast and accurate algorithm for the simulation of NMR spectra of large spin systems.
Fast and accurate algorithm for the simulation of NMR spectra of large spin systems.
Fast and accurate algorithm for the simulation of NMR spectra of large spin systems.
J Magn Reson. 2011 Apr;209(2):123-30
Authors: Castillo AM, Patiny L, Wist J
The computational cost for the simulation of NMR spectra grows exponentially with the number of nuclei. Today, the memory available to store the Hamiltonian limits the size of the system that can be studied. Modern computers enable to tackle systems containing up to 13 spins , which obviously does not...
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07-23-2011 08:54 AM
NMR insights into the core of GED assembly by H/D exchange coupled with DMSO dissociation and analysis of the denatured state.
NMR insights into the core of GED assembly by H/D exchange coupled with DMSO dissociation and analysis of the denatured state.
NMR insights into the core of GED assembly by H/D exchange coupled with DMSO dissociation and analysis of the denatured state.
J Mol Biol. 2011 Feb 4;405(5):1202-14
Authors: Chakraborty S, Hosur RV
GTPase effector domain (GED) of dynamin forms megadalton-sized assembly in vitro, rendering its structural characterization highly challenging. To probe the core of the GED assembly, we performed H/D exchange in native...
Quantitative analysis of 2D EXSY NMR spectra of strongly coupled spin systems in transmembrane exchange
Linear Mode
