Related ArticlesQuantitation of metabolic compartmentation in hyperammonemic brain by natural abundance 13C-NMR detection of 13C-15N coupling patterns and isotopic shifts.
Eur J Biochem. 1997 Feb 1;243(3):597-604
Authors: Lapidot A, Gopher A
In the present study, the removal of cerebral ammonia by glutamine synthetase (GS) and by reductive amination of 2-oxoglutarate by glutamate dehydrogenase in the presence of an amino donor group, was determined in hyperammonemic rabbit brains. The 15N enrichments of brain metabolite alpha-amino and amide positions of glutamine, glutamate, and alanine were determined by the indirect detection of 15N-labeled compounds of the 13C-15N spin coupling patterns of natural abundance 13C-NMR spectra. The 13C-NMR spectra of brain extracts were obtained from rabbits infused with 15NH4Cl with or without intraperitoneal infusion of the GS inhibitor, L-methionine DL-sulfoximine, in a reasonable acquisition time period. When 15NH4Cl was infused, [5-15N]glutamine and [2-15N]glutamine concentrations reached 5.2 mumol/100 mg protein and 3.6 mumol/100 mg protein, respectively, which indicates the relatively high activity of reductive amination of 2-oxoglutarate in the glutamate dehydrogenase reaction. The low concentration of [2-15N]glutamate, which is about 30% of that of [2-15N]glutamine obtained in this study, suggests that very little glutamine serves as a precursor of neuronal glutamate. When GS was inhibited by L-methionine DL-sulfoximine, a flux of 15NH4+ via the residual activity of GS was accompanied by an apparent increase of [2-15N]glutamate and [15N]alanine concentrations (2.9 mumol/100 mg protein and 1.8 mumol/100 mg protein, respectively). These findings and those obtained from 13C-13C isotopomer analysis (Lapidot and Gopher, 1994b) suggest that astrocytic 2-oxoglutarate is partially utilized (together with an amino group donor) as a precursor for neuronal glutamate in the hyperammonemic brain when GS is inhibited. This process can partly replace GS activity in metabolizing ammonia in the hyperammonemic rabbit brain.
Mutations in the Saccharomyces cerevisiae succinate dehydrogenase result in distinct metabolic phenotypes revealed through (1)H NMR-based metabolic footprinting.
Mutations in the Saccharomyces cerevisiae succinate dehydrogenase result in distinct metabolic phenotypes revealed through (1)H NMR-based metabolic footprinting.
Mutations in the Saccharomyces cerevisiae succinate dehydrogenase result in distinct metabolic phenotypes revealed through (1)H NMR-based metabolic footprinting.
J Proteome Res. 2010 Dec 3;9(12):6729-39
Authors: Szeto SS, Reinke SN, Sykes BD, Lemire BD
Metabolomics is a powerful method of examining the intricate connections between mutations, metabolism, and disease. Metabolic...
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05-25-2011 07:01 PM
Use of optimized 1D TOCSY NMR for improved quantitation and metabolomic analysis of biofluids
Use of optimized 1D TOCSY NMR for improved quantitation and metabolomic analysis of biofluids
Abstract One dimensional selective TOCSY experiments have been shown to be advantageous in providing improved data inputs for principle component analysis (PCA) (Sandusky and Raftery 2005a, b). Better subpopulation cluster resolution in the observed scores plots results from the ability to isolate metabolite signals of interest via the TOCSY based filtering approach. This report reexamines the quantitative aspects of this approach, first by optimizing the 1D TOCSY experiment as it relates to...
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03-13-2011 05:24 AM
[Question from NMRWiki Q&A forum] Bruker Tpospin for brain spectroscopy analysis
Bruker Tpospin for brain spectroscopy analysis
Hi I am using Bruker Tpospin for brain spectroscopy analysis. I am new to this software. My question is how do I make spectral lines dotted or thicker instead of colors. Any hint is highly appreciated. ThanksSurya
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03-01-2011 01:51 AM
[NMR paper] Quantitation of protein expression in a cell-free system: Efficient detection of yiel
Quantitation of protein expression in a cell-free system: Efficient detection of yields and 19F NMR to identify folded protein.
Related Articles Quantitation of protein expression in a cell-free system: Efficient detection of yields and 19F NMR to identify folded protein.
J Biomol NMR. 2005 Jan;31(1):11-9
Authors: Neerathilingam M, Greene LH, Colebrooke SA, Campbell ID, Staunton D
We have developed an efficient and novel filter assay method, involving radioactive labelling and imaging, to quantify the expression of soluble proteins from a...
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11-24-2010 11:14 PM
[NMR paper] Quantitation of metabolic compartmentation in hyperammonemic brain by natural abundan
Quantitation of metabolic compartmentation in hyperammonemic brain by natural abundance 13C-NMR detection of 13C-15N coupling patterns and isotopic shifts.
http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--www3.interscience.wiley.com-aboutus-images-wiley_interscience_pubmed_logo_FREE_120x27.gif Related Articles Quantitation of metabolic compartmentation in hyperammonemic brain by natural abundance 13C-NMR detection of 13C-15N coupling patterns and isotopic shifts.
Eur J Biochem. 1997 Feb 1;243(3):597-604
Authors: Lapidot A, Gopher A
In...
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08-22-2010 03:31 PM
[NMR paper] 1H NMR-based absolute quantitation of human lipoproteins and their lipid contents dir
1H NMR-based absolute quantitation of human lipoproteins and their lipid contents directly from plasma.
Related Articles 1H NMR-based absolute quantitation of human lipoproteins and their lipid contents directly from plasma.
J Lipid Res. 1994 Dec;35(12):2292-304
Authors: Ala-Korpela M, Korhonen A, Keisala J, Hörkkö S, Korpi P, Ingman LP, Jokisaari J, Savolainen MJ, Kesäniemi YA
A new method is presented for absolute quantitation of lipid and protein contents of human lipoproteins directly from plasma. The method enables complete lipoprotein...
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08-22-2010 03:29 AM
[NMR paper] In vivo 133Cs-NMR a probe for studying subcellular compartmentation and ion uptake in
In vivo 133Cs-NMR a probe for studying subcellular compartmentation and ion uptake in maize root tissue.
http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--linkinghub.elsevier.com-ihub-images-PubMedLink.gif Related Articles In vivo 133Cs-NMR a probe for studying subcellular compartmentation and ion uptake in maize root tissue.
Biochim Biophys Acta. 1990 Sep 1;1054(2):169-75
Authors: Pfeffer PE, Rolin DB, Brauer D, Tu SI, Kumosinski TF
Three 133Cs-NMR signals were observed in the spectra of CsCl-perfused and CsCl-grown maize seedling root...
Quantitation of metabolic compartmentation in hyperammonemic brain by natural abundan
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