Quantifying protein-drug lifetimes in human cells by (19)F NMR spectroscopy
 

Quantifying protein-drug lifetimes in human cells by (19)F NMR spectroscopy

The cellular environment is a complex and crowded space, with organelles, compartments and multitudes of molecules engaged in intricate networks of communication that modulate binary protein-ligand/protein interactions. As a result, it is becoming increasingly appreciated that evaluations of protein-drug binding should be carried out in the native cellular environment. Here, we present a proof-of-concept study where we measured the lifetime (1/k(off)) of a protein-drug complex in human cells by...

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