High-moisture (HM) extrusion is the dominant industrial process to create structured plant-based protein products that can be used for animal-free meat alternatives. Yet, the underlying mechanisms, such as phase separation, that govern structure formation in plant-protein extrudates, are still poorly understood. Current hypotheses require experimental data in order to be verified, but measurement techniques able to quantify phase-separated anisotropic protein extrudates are lacking, or have yet...
[ASAP] Common Pitfalls and Recommendations for Using a Turbidity Assay to Study Protein Phase Separation
Common Pitfalls and Recommendations for Using a Turbidity Assay to Study Protein Phase Separation
https://pubs.acs.org/na101/home/literatum/publisher/achs/journals/content/bichaw/0/bichaw.ahead-of-print/acs.biochem.1c00386/20210809/images/medium/bi1c00386_0008.gif
Biochemistry
DOI: 10.1021/acs.biochem.1c00386
http://feeds.feedburner.com/~r/acs/bichaw/~4/FWbVq6tkE08
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nmrlearner
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08-09-2021 09:44 PM
[NMR paper] Interaction hot spots for phase separation revealed by NMR studies of a CAPRIN1 condensed phase
Interaction hot spots for phase separation revealed by NMR studies of a CAPRIN1 condensed phase
The role of biomolecular condensates in regulating biological function and the importance of dynamic interactions involving intrinsically disordered protein regions (IDRs) in their assembly are increasingly appreciated. While computational and theoretical approaches have provided significant insights into IDR phase behavior, establishing the critical interactions that govern condensation with atomic resolution through experiment is more difficult, given the lack of applicability of standard...
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nmrlearner
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06-02-2021 05:10 PM
Interaction hot spots for phase separation revealed by NMR studies of a CAPRIN1 condensed phase [Biophysics and Computational Biology]
Interaction hot spots for phase separation revealed by NMR studies of a CAPRIN1 condensed phase
Tae Hun Kim, Brandon J. Payliss, Michael L. Nosella, Ian T. W. Lee, Yuki Toyama, Julie D. Forman-Kay, Lewis E. Kay...
Date: 2021-06-01
The role of biomolecular condensates in regulating biological function and the importance of dynamic interactions involving intrinsically disordered protein regions (IDRs) in their assembly are increasingly appreciated. While computational and theoretical approaches have provided significant insights into IDR phase behavior, establishing the critical...
Quantification of phase separation in high moisture soy protein extrudates by NMR and MRI
Linear Mode
