Related ArticlesQuality Control of Therapeutic Peptides by 1H NMR HiFSA Sequencing.
J Org Chem. 2019 03 15;84(6):3055-3073
Authors: Choules MP, Bisson J, Gao W, Lankin DC, McAlpine JB, Niemitz M, Jaki BU, Franzblau SG, Pauli GF
Abstract
Ensuring identity, purity, and reproducibility are equally essential during synthetic chemistry, drug discovery, and for pharmaceutical product safety. Many peptidic APIs are large molecules that require considerable effort for integrity assurance. This study builds on quantum mechanical 1H iterative Full Spin Analysis (HiFSA) to establish NMR peptide sequencing methodology that overcomes the intrinsic limitations of principal compendial methods in identifying small structural changes or minor impurities that affect effectiveness and safety. HiFSA sequencing yields definitive identity and purity information concurrently, allowing for API quality assurance and control (QA/QC). Achieving full peptide analysis via NMR building blocks, the process lends itself to both research and commercial applications as 1D 1H NMR (HNMR) is the most sensitive and basic NMR experiment. The generated HiFSA profiles are independent of instrument or software tools and work at any magnetic field strength. Pairing with absolute or 100% qHNMR enables quantification of mixtures and/or determination of peptide conformer populations. Demonstration of the methodology uses single amino acids (AAs) and peptides of increasing size, including the octapeptide, angiotensin II, and the nonapeptide, oxytocin. The feasibility of HiFSA coupled with automated NMR and qHNMR for use in QC/QA efforts is established through case-based examples and recommended procedures.
NMR quality control of fragment libraries for screening
NMR quality control of fragment libraries for screening
Abstract
Fragment-based screening has evolved as a remarkable approach within the drug discovery process both in the industry and academia. Fragment screening has become a more structure-based approach to inhibitor development, but also towards development of pathway-specific clinical probes. However, it is often witnessed that the availability, immediate and long-term, of a high quality fragment-screening library is still beyond the reach of most academic laboratories. Within iNEXT...
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06-13-2020 05:51 PM
Structural investigation of glycan recognition by the ERAD quality control lectin Yos9
Structural investigation of glycan recognition by the ERAD quality control lectin Yos9
Abstract
Yos9 is an essential component of the endoplasmic reticulum associated protein degradation (ERAD) system that is responsible for removing terminally misfolded proteins from the ER lumen and mediating proteasomal degradation in the cytosol. Glycoproteins that fail to attain their native conformation in the ER expose a distinct oligosaccharide structure, a terminal α1,6-linked mannose residue, that is specifically recognized by the mannose 6-phoshate...
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11-25-2018 06:02 AM
Fluorine pharma: Quality control by NMR
Fluorine pharma: Quality control by NMR
http://www.spectroscopynow.com/common/images/thumbnails/1600c98c38f.jpgRoutine analysis and quality control of fluorine-containing pharmaceuticals could now be possible with the application of the relatively simple and straightforward technique of fluorine-19 nuclear magnetic resonance (NMR) spectroscopy, according to researchers in Kenya and Germany.
Read the rest at Spectroscopynow.com
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12-01-2017 08:23 AM
Low-Field Permanent Magnets for Industrial Process and Quality Control
Low-Field Permanent Magnets for Industrial Process and Quality Control
Publication date: Available online 30 September 2013
Source:Progress in Nuclear Magnetic Resonance Spectroscopy</br>
Author(s): J. Mitchell , L.F. Gladden , T.C. Chandrasekera , E.J. Fordham</br>
In this review we focus on the technology associated with lowfield NMR. We present the current state-of-the-art in low-field NMR hardware and experiments, considering general magnet designs, rf performance, data processing and interpretation. We provide guidance on obtaining the optimum results from...
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09-30-2013 03:48 PM
[NMR paper] Heteronuclear NMR provides an accurate assessment of therapeutic insulin's quality.
Heteronuclear NMR provides an accurate assessment of therapeutic insulin's quality.
Related Articles Heteronuclear NMR provides an accurate assessment of therapeutic insulin's quality.
J Pharm Biomed Anal. 2013 Feb 20;78-79C:252-254
Authors: Quinternet M, Starck JP, Delsuc MA, Kieffer B
Abstract
New generations of drugs are using more and more often therapeutic proteins as the active ingredient, prompting the regulation agencies to adapt their analytical methods. Fast and unambiguous information on the secondary, tertiary and quaternary...
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03-19-2013 01:22 PM
Real-time NMR Studies of the Folding & Mechanisms of Protein Quality Control Machiner
Real-time NMR Studies of the Folding & Mechanisms of Protein Quality Control Machineries
* In the context of a project funded by European Research Council, two postdoctoral positions are available at the Structural Biology Institute in Grenoble (France) to study by real-time NMR the Folding & Mechanisms of Protein Quality Control (PQC) Machineries. Selected candidates will use latest NMR technologies developed at IBS to characterize self-assembly and functionally important structural rearrangements of large PQC machineries isolated at IBS.
* The laboratory host…
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Quality Control of Therapeutic Peptides by 1H NMR HiFSA Sequencing.
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