Pulse design for broadband correlation NMR spectroscopy by multi-rotating frames.
J Biomol NMR. 2013 Feb 19;
Authors: Coote P, Arthanari H, Yu TY, Natarajan A, Wagner G, Khaneja N
Abstract
We present a method for designing radio-frequency (RF) pulses for broadband or multi-band isotropic mixing at low power, suitable for protein NMR spectroscopy. These mixing pulses are designed analytically, rather than by numerical optimization, by repeatedly constructing new rotating frames of reference. We show how pulse parameters can be chosen frame-by-frame to systematically reduce the effective chemical shift bandwidth, but maintain most of the effective J-coupling strength. The effective Hartmann-Hahn mixing condition is then satisfied in a multi-rotating frame of reference. This design method yields multi-band and broadband mixing pulses at low RF power. In particular, the ratio of RF power to mixing bandwidth for these pulses is lower than for existing mixing pulses, such as DIPSI and FLOPSY. Carbon-carbon TOCSY experiments at low RF power support our theoretical analysis.
PMID: 23420125 [PubMed - as supplied by publisher]
[Question from NMRWiki Q&A forum] Pulse sequence for homonuclear correlation with SPC5 mixing period
Pulse sequence for homonuclear correlation with SPC5 mixing period
Hi,I'm looking for a pulse program suitable for Bruker Avance llI (topspin 2.1) which is 2D homonuclear correlation with a mixing period of the symmetry based pulse scheme - the SPC5 scheme. I took the basic PDSD experiment and just replaced the mixing period with the appropriate pulses of the SPC5 scheme but it didn't work. Does someone has a ready-to-run pulse scheme out there? Basically for 13C-13C correlation, the program starts with a 90 pulse on 1H, followed by CP from 1H to 13C, t1 evolution, 90 pulse on 13C, mixing...
Uniform broadband excitation of crystallites in rotating solids using interleaved sequences of delays alternating with nutation
Uniform broadband excitation of crystallites in rotating solids using interleaved sequences of delays alternating with nutation
Publication year: 2012
Source:Journal of Magnetic Resonance</br>
Veronika Vitzthum, Marc A. Caporini, Simone Ulzega, Julien Trébosc, Olivier Lafon, Jean-Paul Amoureux, Geoffrey Bodenhausen</br>
In solids that are spinning about the magic angle, trains of short pulses in the manner of Delays Alternating with Nutations for Tailored Excitation (DANTE) allow one to improve the efficiency of the excitation of magnetization compared to...
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06-13-2012 11:34 AM
[Stan NMR blog] Rotating Molecules versus Rotating Spins
Rotating Molecules versus Rotating Spins
Clarification of a confusing aspect of NMR
Source: Stan blog library
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03-26-2012 07:34 PM
[Stan NMR blog] Rotating Molecules versus Rotating Spins
Rotating Molecules versus Rotating Spins
Clarification of a confusing aspect of NMR
Source: Stan blog library
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01-11-2011 01:14 PM
Fluorine-Protein Interactions and (19)F NMR Isotropic Chemical Shifts: An Empirical Correlation with Implications for Drug Design.
Fluorine-Protein Interactions and (19)F NMR Isotropic Chemical Shifts: An Empirical Correlation with Implications for Drug Design.
Related Articles Fluorine-Protein Interactions and (19)F NMR Isotropic Chemical Shifts: An Empirical Correlation with Implications for Drug Design.
ChemMedChem. 2010 Nov 29;
Authors: Dalvit C, Vulpetti A
An empirical correlation between the fluorine isotropic chemical shifts, measured by (19)F NMR spectroscopy, and the type of fluorine-protein interactions observed in crystal structures is presented. The CF, CF(2), and...
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12-01-2010 04:41 PM
[NMR paper] Three-dimensional electrophoretic NMR correlation spectroscopy.
Three-dimensional electrophoretic NMR correlation spectroscopy.
Related Articles Three-dimensional electrophoretic NMR correlation spectroscopy.
J Magn Reson. 2000 Dec;147(2):361-5
Authors: He Q, Lin W, Liu Y, Li E
A novel method of three-dimensional electrophoretic NMR correlation spectroscopy (3D EP-COSY) has been proposed, developed, and implemented. It has a demonstrated potential of facilitating simultaneous structural assignments of multiple proteins in mixtures. The principle is to add a pulsed DC electric field that introduces a new...
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11-19-2010 08:29 PM
Broadband 15Nâ??13C dipolar recoupling via symmetry-based RF pulse schemes at high MA
Abstract An approach for generating efficient
RNnnS, nk symmetry-based dual channel RF pulse schemes for γ-encoded broadband 15Nâ??13C dipolar recoupling at high magic angle spinning frequencies is presented. The method involves the numerical optimisation of the RF phase-modulation profile of the basic â??Râ?? element so as to obtain heteronuclear double quantum dipolar recoupling sequences with satisfactory magnetisation transfer characteristics. The basic â??Râ?? element was implemented as a sandwich of a small number of short pulses of equal duration with each pulse characterised by...
Pulse design for broadband correlation NMR spectroscopy by multi-rotating frames.
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