Pruning the ALS-Associated Protein SOD1 for in-CellNMR

		BioNMR > Educational resources > Journal club
Pruning the ALS-Associated Protein SOD1 for in-CellNMR

Webservers

NMR processing:

MDD

NMR assignment:

Backbone:

Side-chains:

NOEs:

Structure from NMR restraints:

Ab initio:

Fragment-based:

Rosetta-NMR (Robetta)

Template-based:

GeNMR

I-TASSER

Refinement:

Amber

Structure from chemical shifts:

Fragment-based:

Homology-based:

Torsion angles from chemical shifts:

Preditor

TALOS

Promega- Proline

Secondary structure from chemical shifts:

CSI (via RCI server)

TALOS

MICS caps, β-turns

d2D

PECAN

Flexibility from chemical shifts:

RCI

Interactions from chemical shifts:

HADDOCK

Chemical shifts re-referencing:

NMR model quality:

NOEs, other restraints:

Chemical shifts:

RDCs:

Pseudocontact shifts:

Protein geomtery:

SAVES2 or SAVES4

Quality Control Check

NMR spectrum prediction:

FANDAS

MestReS

V-NMR

Flexibility from structure:

Backbone S2

Methyl S2

B-factor

Molecular dynamics:

Gromacs

Amber

Antechamber

Chemical shifts prediction:

From structure:

Shiftx2

Sparta+

Camshift

CH3shift- Methyl

ArShift- Aromatic

ShiftS

Proshift

PPM

CheShift-2- Cα

From sequence:

Shifty

Camcoil

Poulsen_rc_CS

Disordered proteins:

MAXOCC

Format conversion & validation:

CCPN

From NMR-STAR 3.1

Validate NMR-STAR 3.1

NMR sample preparation:

Protein disorder:

DisMeta

Protein solubility:

Isotope labeling:

Solid-state NMR:

Thread Tools

Search this Thread

Rate Thread

Display Modes

07-09-2013, 02:41 AM

nmrlearner

Senior Member

Join Date: Jan 2005

Posts: 23,777

Points: 193,617, Level: 100

Level up: 0%, 0 Points needed

Activity: 50.7%

Last Achievements

Award-Showcase

NMR Credits: 0

NMR Points: 193,617

Downloads: 0

Uploads: 0

Pruning the ALS-Associated Protein SOD1 for in-CellNMR

Pruning the ALS-Associated Protein SOD1 for in-CellNMR

Jens Danielsson, Kohsuke Inomata, Shuhei Murayama, Hidehito Tochio, Lisa Lang, Masahiro Shirakawa and Mikael Oliveberg

Journal of the American Chemical Society
DOI: 10.1021/ja404425r

Source: Journal of the American Chemical Society

Did you find this post helpful?

Similar Threads
Thread	Thread Starter	Forum	Replies	Last Post
[NMR paper] Pruning the ALS-associated protein SOD1 for in-cell NMR. Pruning the ALS-associated protein SOD1 for in-cell NMR. Related Articles Pruning the ALS-associated protein SOD1 for in-cell NMR. J Am Chem Soc. 2013 Jul 2; Authors: Danielsson J, Inomata K, Murayama S, Tochio H, Lang L, Shirakawa M, Oliveberg M Abstract To efficiently deliver isotope-labelled proteins into mammalian cells poses a main challenge for structural and functional analysis by in-cell NMR. In this study we have employed cell-penetrating peptides (CPPs) to transfer the ALS-associated protein superoxide dismutase (SOD1) into...	nmrlearner	Journal club	0	07-05-2013 08:03 AM

« Previous Thread | Next Thread »

Posting Rules
You may not post new threads You may not post replies You may not post attachments You may not edit your posts BB code is On Smilies are On [IMG] code is On HTML code is On Trackbacks are Off Pingbacks are Off Refbacks are Off