Related ArticlesProton NMR spectroscopy shows lipids accumulate in skeletal muscle in response to burn trauma-induced apoptosis.
FASEB J. 2005 Sep;19(11):1431-40
Authors: Astrakas LG, Goljer I, Yasuhara S, Padfield KE, Zhang Q, Gopalan S, Mindrinos MN, Dai G, Yu YM, Martyn JA, Tompkins RG, Rahme LG, Tzika AA
Burn trauma triggers hypermetabolism and muscle wasting via increased cellular protein degradation and apoptosis. Proton nuclear magnetic resonance (1H NMR) spectroscopy can detect mobile lipids in vivo. To examine the local effects of burn in skeletal muscle, we performed in vivo 1H NMR on mice 3 days after burn trauma; and ex vivo, high-resolution, magic angle spinning (1)H NMR on intact excised mouse muscle samples before and 1 and 3 days after burn. These samples were then analyzed for apoptotic nuclei using a terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling (TUNEL) assay. To confirm our NMR and cell biology results, we used transcriptome analysis to demonstrate that burn trauma alters the expression of genes involved in lipid metabolism and apoptosis. Our results demonstrate that burn injury results in a localized intramyocellular lipid accumulation, which in turn is accompanied by burn-induced apoptosis and mitochondrial dysfunction, as seen by the up-regulation of apoptotic genes and down-regulation of genes that encode lipid oxidation and the peroxisomal proliferator activator receptor gamma coactivator PGC-1beta. Moreover, the increased levels of bisallylic methylene fatty acyl protons (2.8 ppm) and vinyl protons (5.4 ppm), in conjunction with the TUNEL assay results, further suggest that burn trauma results in apoptosis. Together, our results provide new insight into the local physiological changes that occur in skeletal muscle after severe burn trauma.
Skeletal muscle lipid metabolism studied by advanced magnetic resonance spectroscopy
Skeletal muscle lipid metabolism studied by advanced magnetic resonance spectroscopy
Publication year: 2012
Source: Progress in Nuclear Magnetic Resonance Spectroscopy, Available online 23 February 2012</br>
Arunima*Pola, Suresh Anand*Sadananthan, Jadegoud*Yaligar, Vijayasarathi*Nagarajan, Weiping*Han, ...</br>
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Effect of ischemic preconditioning in skeletal muscle measured by functional ... - 7thSpace Interactive (press release)
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Effect of ischemic preconditioning in skeletal muscle measured by functional ...
7thSpace Interactive (press release)
Nuclear magnetic resonance (NMR) imaging and spectroscopy have been applied to assess skeletal muscle oxidative metabolism. Therefore, in-vivo NMR may enable the characterization of ischemia-reperfusion injury. The goal of this study was to evaluate ...
Protein NMR Spectroscopy: Practical Techniques and ApplicationsspectroscopyNOW.com
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Effect of ischemic preconditioning in skeletal muscle measured by...
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[NMR paper] (1)H/(15)N heteronuclear NMR spectroscopy shows four dynamic domains for phospholamba
(1)H/(15)N heteronuclear NMR spectroscopy shows four dynamic domains for phospholamban reconstituted in dodecylphosphocholine micelles.
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Biophys J. 2004 Aug;87(2):1205-14
Authors: Metcalfe EE, Zamoon J, Thomas DD, Veglia G
We report the backbone dynamics of monomeric phospholamban in dodecylphosphocholine micelles using (1)H/(15)N heteronuclear NMR spectroscopy. Phospholamban is a 52-amino acid...
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[NMR paper] 13C/31P NMR assessment of mitochondrial energy coupling in skeletal muscle of awake f
13C/31P NMR assessment of mitochondrial energy coupling in skeletal muscle of awake fed and fasted rats. Relationship with uncoupling protein 3 expression.
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J Biol Chem. 2000 Dec 15;275(50):39279-86
Authors: Jucker BM, Ren J, Dufour S, Cao X, Previs SF, Cadman KS, Shulman GI
To examine the relationship between mitochondrial energy coupling in skeletal muscle and change in...
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[NMR paper] 1H-NMR and raman studies on perforating trauma-induced cataract formation in a mouse
1H-NMR and raman studies on perforating trauma-induced cataract formation in a mouse lens.
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Biochim Biophys Acta. 2000 Mar 6;1474(1):23-30
Authors: Nakamura K, Jung YM, Era S, Sogami M, Ozaki Y, Takasaki A
In order to provide new insight into the molecular mechanism of perforating trauma-induced cataract formation in an 8-week-old ddY mouse lens, we performed an in situ investigation into changes in the water-protein and/or...
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[NMR paper] NMR solution structure of calcium-saturated skeletal muscle troponin C.
NMR solution structure of calcium-saturated skeletal muscle troponin C.
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Biochemistry. 1995 Dec 12;34(49):15953-64
Authors: Slupsky CM, Sykes BD
Troponin C (TnC) is an 18 kDa (162-residue) thin-filament calcium-binding protein responsible for triggering muscle contraction upon the release of calcium from the sarcoplasmic reticulum. The structure of TnC with two calcium ions bound has previously been solved by X-ray methods. Shown here is the solution...
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[NMR paper] Cephaloridine-induced nephrotoxicity in the Fischer 344 rat: proton NMR spectroscopic
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Arch Toxicol. 1992;66(8):525-37
Authors: Anthony ML, Gartland KP, Beddell CR, Lindon JC, Nicholson JK
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