Proton NMR Based Investigation of the Effects of Temperature and NaCl on Micellar Properties of CHAPS.

		BioNMR > Educational resources > Journal club
Proton NMR Based Investigation of the Effects of Temperature and NaCl on Micellar Properties of CHAPS.

Webservers

NMR processing:

MDD

NMR assignment:

Backbone:

Side-chains:

NOEs:

Structure from NMR restraints:

Ab initio:

Fragment-based:

Rosetta-NMR (Robetta)

Template-based:

GeNMR

I-TASSER

Refinement:

Amber

Structure from chemical shifts:

Fragment-based:

Homology-based:

Torsion angles from chemical shifts:

Preditor

TALOS

Promega- Proline

Secondary structure from chemical shifts:

CSI (via RCI server)

TALOS

MICS caps, β-turns

d2D

PECAN

Flexibility from chemical shifts:

RCI

Interactions from chemical shifts:

HADDOCK

Chemical shifts re-referencing:

NMR model quality:

NOEs, other restraints:

Chemical shifts:

RDCs:

Pseudocontact shifts:

Protein geomtery:

SAVES2 or SAVES4

Quality Control Check

NMR spectrum prediction:

FANDAS

MestReS

V-NMR

Flexibility from structure:

Backbone S2

Methyl S2

B-factor

Molecular dynamics:

Gromacs

Amber

Antechamber

Chemical shifts prediction:

From structure:

Shiftx2

Sparta+

Camshift

CH3shift- Methyl

ArShift- Aromatic

ShiftS

Proshift

PPM

CheShift-2- Cα

From sequence:

Shifty

Camcoil

Poulsen_rc_CS

Disordered proteins:

MAXOCC

Format conversion & validation:

CCPN

From NMR-STAR 3.1

Validate NMR-STAR 3.1

NMR sample preparation:

Protein disorder:

DisMeta

Protein solubility:

Isotope labeling:

Solid-state NMR:

Thread Tools

Search this Thread

Rate Thread

Display Modes

02-17-2011, 07:58 PM

nmrlearner

Senior Member

Join Date: Jan 2005

Posts: 23,777

Points: 193,617, Level: 100

Level up: 0%, 0 Points needed

Activity: 50.7%

Last Achievements

Award-Showcase

NMR Credits: 0

NMR Points: 193,617

Downloads: 0

Uploads: 0

Proton NMR Based Investigation of the Effects of Temperature and NaCl on Micellar Properties of CHAPS.

Proton NMR Based Investigation of the Effects of Temperature and NaCl on Micellar Properties of CHAPS.

Proton NMR Based Investigation of the Effects of Temperature and NaCl on Micellar Properties of CHAPS.

J Phys Chem B. 2011 Feb 15;

Authors: Qin X, Liu M, Zhang X, Yang D

The effects of temperature and NaCl on the micellization of CHAPS, a zwitterionic detergent widely used in membrane protein studies, have been investigated by NMR spectroscopy. We found that the two apparent critical micelle concentration (cmc) values of CHAPS decrease with the increase of temperature, as well as the NaCl concentration. The thermodynamic parameters derived from the temperature-dependent cmc values show that the micellization process is spontaneous and exothermic, and the van der Waals interaction is likely to be the main factor for the micellization of CHAPS. The micellar hydrodynamic radii remain almost the same in a range of 100-600 mM NaCl, indicating that the aggregate states of CHAPS are not sensitive to the change of the surrounding conditions. In addition, the dependence of nuclear Overhauser effect (NOE) intensities on temperatures further demonstrates the existence of the unique staggered micellar structure of CHAPS at a concentration above the apparent second cmc, which was suggested in our previous work. Our results provide a basis for optimizing CHAPS concentration in the solubilization or stabilization of membrane proteins under nondenaturing conditions and may be helpful to understand its interaction with proteins.

PMID: 21322620 [PubMed - as supplied by publisher]

Source: PubMed

Did you find this post helpful?

Similar Threads
Thread	Thread Starter	Forum	Replies	Last Post
Cryogenic temperature effects and resolution upon slow cooling of protein preparations in solid state NMR Cryogenic temperature effects and resolution upon slow cooling of protein preparations in solid state NMR Abstract X-ray crystallography using synchrotron radiation and the technique of dynamic nuclear polarization (DNP) in nuclear magnetic resonance (NMR) require samples to be kept at temperatures below 100 K. Protein dynamics are poorly understood below the freezing point of water and down to liquid nitrogen temperatures. Therefore, we investigate the Î±-spectrin SH3 domain by magic angle spinning (MAS) solid state NMR (ssNMR) at various temperatures while cooling slowly. Cooling down...	nmrlearner	Journal club	0	08-13-2011 02:47 AM
Cryogenic temperature effects and resolution upon slow cooling of protein preparations in solid state NMR. Cryogenic temperature effects and resolution upon slow cooling of protein preparations in solid state NMR. Cryogenic temperature effects and resolution upon slow cooling of protein preparations in solid state NMR. J Biomol NMR. 2011 Aug 9; Authors: Linden AH, Franks WT, Akbey U, Lange S, van Rossum BJ, Oschkinat H X-ray crystallography using synchrotron radiation and the technique of dynamic nuclear polarization (DNP) in nuclear magnetic resonance (NMR) require samples to be kept at temperatures below 100*K. Protein dynamics are poorly understood...	nmrlearner	Journal club	0	08-10-2011 12:30 PM
Random coil chemical shift for intrinsically disordered proteins: effects of temperature and pH Random coil chemical shift for intrinsically disordered proteins: effects of temperature and pH Abstract Secondary chemical shift analysis is the main NMR method for detection of transiently formed secondary structure in intrinsically disordered proteins. The quality of the secondary chemical shifts is dependent on an appropriate choice of random coil chemical shifts. We report random coil chemical shifts and sequence correction factors determined for a GGXGG peptide series following the approach of Schwarzinger et al. (J Am Chem Soc 123(13):2970â??2978, 2001). The chemical shifts are...	nmrlearner	Journal club	0	01-17-2011 02:40 AM
[NMR paper] Temperature-dependence of protein hydrogen bond properties as studied by high-resolut Temperature-dependence of protein hydrogen bond properties as studied by high-resolution NMR. Related Articles Temperature-dependence of protein hydrogen bond properties as studied by high-resolution NMR. J Mol Biol. 2002 Apr 12;317(5):739-52 Authors: Cordier F, Grzesiek S The temperature-dependence of a large number of NMR parameters describing hydrogen bond properties in the protein ubiquitin was followed over a range from 5 to 65 degrees C. The parameters comprise hydrogen bond (H-bond) scalar couplings, h3JNC', chemical shifts, amide...	nmrlearner	Journal club	0	11-24-2010 08:49 PM
[NMR paper] Solid-state (13)C NMR reveals effects of temperature and hydration on elastin. Solid-state (13)C NMR reveals effects of temperature and hydration on elastin. Related Articles Solid-state (13)C NMR reveals effects of temperature and hydration on elastin. Biophys J. 2002 Feb;82(2):1086-95 Authors: Perry A, Stypa MP, Tenn BK, Kumashiro KK Elastin is the principal protein component of the elastic fiber in vertebrate tissue. The waters of hydration in the elastic fiber are believed to play a critical role in the structure and function of this largely hydrophobic, amorphous protein. (13)C CPMAS NMR spectra are acquired for...	nmrlearner	Journal club	0	11-24-2010 08:49 PM
Investigation of the dynamical properties of water in elastin by deuterium Double Qua Investigation of the dynamical properties of water in elastin by deuterium Double Quantum Filtered NMR. http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--linkinghub.elsevier.com-ihub-images-PubMedLink.gif Related Articles Investigation of the dynamical properties of water in elastin by deuterium Double Quantum Filtered NMR. J Magn Reson. 2010 Jul;205(1):86-92 Authors: Sun C, Boutis GS The anisotropic motion of tightly bound waters of hydration in bovine nuchal ligament elastin has been studied by deuterium Double Quantum Filtered (DQF) NMR....	nmrlearner	Journal club	0	09-22-2010 05:27 AM
[NMR paper] The effects of temperature, viscosity, and molecular size on the aluminum-27 QCT NMR The effects of temperature, viscosity, and molecular size on the aluminum-27 QCT NMR of transferrins. Related Articles The effects of temperature, viscosity, and molecular size on the aluminum-27 QCT NMR of transferrins. J Magn Reson B. 1996 Feb;110(2):182-7 Authors: Aramini JM, Vogel HJ A number of reports in recent years have demonstrated the feasibility of detecting quadrupolar metal ions bound tightly to rather large proteins via the quadrupolar central transition (QCT) NMR approach. In this article, an in-depth investigation of several...	nmrlearner	Journal club	0	08-22-2010 02:27 PM
[NMR paper] 1H- and 13C-NMR investigation of redox-state-dependent and temperature-dependent conf 1H- and 13C-NMR investigation of redox-state-dependent and temperature-dependent conformation changes in horse cytochrome c. http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--www3.interscience.wiley.com-aboutus-images-wiley_interscience_pubmed_logo_FREE_120x27.gif Related Articles 1H- and 13C-NMR investigation of redox-state-dependent and temperature-dependent conformation changes in horse cytochrome c. Eur J Biochem. 1993 Feb 1;211(3):555-62 Authors: Turner DL, Williams RJ The redox-state dependent changes in chemical shift, which have...	nmrlearner	Journal club	0	08-21-2010 11:53 PM

« Previous Thread | Next Thread »

Posting Rules
You may not post new threads You may not post replies You may not post attachments You may not edit your posts BB code is On Smilies are On [IMG] code is On HTML code is On Trackbacks are Off Pingbacks are Off Refbacks are Off