Publication date: Available online 24 December 2014 Source:Progress in Nuclear Magnetic Resonance Spectroscopy
Author(s): Zhengchao Dong
Water suppression (WS) techniques have played a vital role in the commencement and development of in vivo proton magnetic resonance spectroscopy (MRS, including spectroscopic imaging – MRSI). WS not only made in vivo proton MRS functionally available but also made its applications conveniently accessible, and it has become an indispensable tool in most of the routine applications of in vivo proton MR spectroscopy. On the other hand, WS brought forth some challenges. Therefore, various techniques of proton MRS without WS have been developed since the pioneering work in the late 1990s. After more than one and a half decades of advances in both hardware and software, non-water-suppressed proton MRS is coming to the stage of maturity and seeing increasing application in biomedical research and clinical diagnosis. In this article, we will review progress in the technical development and applications of proton MRS without WS. Graphical abstract
[Question from NMRWiki Q&A forum] 1D NOESY with selective excitation and water suppression
1D NOESY with selective excitation and water suppression
Hi,
I am trying to collect 1D NOESY spectrum of a small protein in 90% H2O with selective excitation so I could follow a few NOEs without running a whole 2D experiment. Is there any sequence in Varian BioPack that I could use for that? I cannot achieve water suppression with the standard 'Noesy1D' sequence and all the other seem to not allow to selectively irradiate only one peak. Maybe it is just a matter of right parameters but I don't know how to set them properly - if so I will appreciate any advice.
Thanks
nmrlearner
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09-11-2013 09:15 PM
[Question from NMRWiki Q&A forum] WEFT water suppression on Varian?
WEFT water suppression on Varian?
I am working with carbohydrates and in 1D NMR we have anomeric protons very close to the HOD peak, sometimes even right under there. In the lab where I used to work we had a bruker machine with an adapted WEFT pulse (described by Hard et al, 1995), which basically does a 180 degree pulse on the HOD, followed by a HS pulse of 50 usec, d1 times to have 0 magnetization on HOD, then a detection pulse.
Since the HOD relaxes much slower than the glycan H-C protons you get about 80% signal on nearby glycan protons and your HOD is usually nearly gone. Glycan...
nmrlearner
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04-11-2013 09:27 PM
NMR Detection of pH-Dependent Histidine–Water Proton Exchange Reveals the Conduction Mechanism of a Transmembrane Proton Channel
NMR Detection of pH-Dependent Histidine–Water Proton Exchange Reveals the Conduction Mechanism of a Transmembrane Proton Channel
Fanghao Hu, Klaus Schmidt-Rohr and Mei Hong
http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jacsat/0/jacsat.ahead-of-print/ja2081185/aop/images/medium/ja-2011-081185_0008.gif
Journal of the American Chemical Society
DOI: 10.1021/ja2081185
http://feeds.feedburner.com/~ff/acs/jacsat?d=yIl2AUoC8zA
http://feeds.feedburner.com/~r/acs/jacsat/~4/C3pPoB5_PR8
nmrlearner
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10-22-2011 10:16 AM
[Question from NMRWiki Q&A forum] Which are better methods of water suppression at higher salt concentrations?
Which are better methods of water suppression at higher salt concentrations?
Hello, could anyone recommend better technique for water suppression for the samples having higher salt concentration?
At what salt concentration is the method effective?
Dr. Talluri mentioned in his post that water suppression using soft pulses (such as one using excitation sculpting) is not as friendly for high salt samples - what is the mechanism?
nmrlearner
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08-22-2010 02:30 AM
[NMRwiki tweet] nmrwiki: Unanswered question: For salty #NMR samples - which water suppression is bes
nmrwiki: Unanswered question: For salty #NMR samples - which water suppression is best? http://qa.nmrwiki.org/question/132/
nmrwiki: Unanswered question: For salty #NMR samples - which water suppression is best? http://qa.nmrwiki.org/question/132/
Source: NMRWiki tweets
nmrlearner
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08-22-2010 01:49 AM
[NMR paper] Proton NMR studies on ischemic rat brain tissue.
Proton NMR studies on ischemic rat brain tissue.
Related Articles Proton NMR studies on ischemic rat brain tissue.
Magn Reson Med. 1992 May;25(1):78-84
Authors: Iwama T, Yamada H, Andoh T, Sakai N, Era S, Sogami M, Kuwata K, Watari H
The spin-lattice relaxation time (T1) of water protons and the cross-relaxation time (TIS) between irradiated protein protons and observed water protons were measured in order to study water-macromolecular interactions in ischemic rat brain tissues. Tissues were obtained by bilateral common carotid artery...
nmrlearner
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08-21-2010 11:41 PM
[NMR paper] 1H-NMR studies on water structures in the rat brain tissues with cerebral tumour or i
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Related Articles 1H-NMR studies on water structures in the rat brain tissues with cerebral tumour or ischaemia.
Acta Neurochir Suppl (Wien). 1990;51:125-7
Authors: Iwama T, Andoh T, Sakai N, Yamada H, Era S, Kuwata K, Sogami M, Watari H
Spin-lattice relaxation times (T1) of water protons and cross-relaxation times (T(IS)) between irradiated protein protons and observed water protons were measured to study water structure in various rat brain tissue....
Proton MRS and MRSI of the Brain without Water Suppression
Linear Mode
