Related ArticlesProtocols for Studying the Interaction of MSI-78 with the Membranes of Whole Gram-Positive and Gram-Negative Bacteria by NMR.
Methods Mol Biol. 2017;1548:217-230
Authors: Santisteban NP, Morrow MR, Booth V
Abstract
Antimicrobial peptides (AMPs) may interact with a variety of target cell components, including the lipid bilayer, non-lipidic cell envelope components, and/or intracellular targets. However, most biophysical experiments aimed at elucidating the detailed mechanism of AMPs are limited to simple model membrane systems and neglect potentially functional interactions between AMPs and non-lipidic cell components. One of the biophysical techniques commonly used to study how AMPs interact with lipid bilayers is solid-state deuterium NMR. In this chapter we provide protocols to prepare deuterium-labeled intact Gram-negative and Gram-positive bacteria and to observe these samples using solid-state deuterium NMR. Such experiments have the potential to provide important information about how non-lipidic cell envelope components modulate AMP interactions with the cytoplasmic membrane of bacteria.
[NMR paper] Preparation of Membrane Models of Gram-Negative Bacteria and Their Interaction with Antimicrobial Peptides Studied by CD and NMR.
Preparation of Membrane Models of Gram-Negative Bacteria and Their Interaction with Antimicrobial Peptides Studied by CD and NMR.
http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--production.springer.de-OnlineResources-Logos-springerlink.gif Related Articles Preparation of Membrane Models of Gram-Negative Bacteria and Their Interaction with Antimicrobial Peptides Studied by CD and NMR.
Methods Mol Biol. 2017;1548:231-245
Authors: Hicks R
Abstract
The antibiotic activity of antimicrobial peptides is generally...
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02-14-2018 02:43 AM
[NMR paper] Studying the assembly of the BAM complex in native membranes by cellular solid-state NMR spectroscopy.
Studying the assembly of the BAM complex in native membranes by cellular solid-state NMR spectroscopy.
Studying the assembly of the BAM complex in native membranes by cellular solid-state NMR spectroscopy.
J Struct Biol. 2017 Nov 29;:
Authors: Pinto C, Mance D, Julien M, Daniels M, Weingarth M, Baldus M
Abstract
Significant progress has been made in obtaining a structural insight into the assembly of the ?-barrel assembly machinery complex (BAM). These crystallography and electron microscopy studies used detergent as a membrane...
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12-05-2017 07:35 PM
[NMR paper] Interaction of the antimicrobial peptides caerin 1.1 and aurein 1.2 with intact bacteria by (2)H solid-state NMR.
Interaction of the antimicrobial peptides caerin 1.1 and aurein 1.2 with intact bacteria by (2)H solid-state NMR.
http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--linkinghub.elsevier.com-ihub-images-PubMedLink.gif Related Articles Interaction of the antimicrobial peptides caerin 1.1 and aurein 1.2 with intact bacteria by (2)H solid-state NMR.
Biochim Biophys Acta. 2016 12;1858(12):2959-2964
Authors: Laadhari M, Arnold AA, Gravel AE, Separovic F, Marcotte I
Abstract
Nuclear magnetic resonance (NMR) is...
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10-28-2017 08:03 AM
[NMR paper] Structural basis of a temporin 1b analogue antimicrobial activity against Gram negative bacteria determined by CD and NMR techniques in cellular environment.
Structural basis of a temporin 1b analogue antimicrobial activity against Gram negative bacteria determined by CD and NMR techniques in cellular environment.
http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--pubs.acs.org-images-pubmed-acspubs.jpg Related Articles Structural basis of a temporin 1b analogue antimicrobial activity against Gram negative bacteria determined by CD and NMR techniques in cellular environment.
ACS Chem Biol. 2015 Apr 17;10(4):965-9
Authors: Malgieri G, Avitabile C, Palmieri M, D'Andrea LD, Isernia C,...
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05-04-2016 01:47 PM
[NMR paper] Peptidoglycan architecture of Gram-positive bacteria by solid-state NMR.
Peptidoglycan architecture of Gram-positive bacteria by solid-state NMR.
http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--linkinghub.elsevier.com-ihub-images-PubMedLink.gif Related Articles Peptidoglycan architecture of Gram-positive bacteria by solid-state NMR.
Biochim Biophys Acta. 2015 Jan;1848(1 Pt B):350-62
Authors: Kim SJ, Chang J, Singh M
Abstract
Peptidoglycan is an essential component of cell wall in Gram-positive bacteria with unknown architecture. In this review, we summarize solid-state NMR...
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02-13-2015 05:31 PM
Conformation and Lipid Interaction of the Fusion Peptide of the Paramyxovirus PIV5 in Anionic and Negative-Curvature Membranes from Solid-State NMR
Conformation and Lipid Interaction of the Fusion Peptide of the Paramyxovirus PIV5 in Anionic and Negative-Curvature Membranes from Solid-State NMR
Hongwei Yao and Mei Hong
http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jacsat/0/jacsat.ahead-of-print/ja4121956/aop/images/medium/ja-2013-121956_0011.gif
Journal of the American Chemical Society
DOI: 10.1021/ja4121956
http://feeds.feedburner.com/~ff/acs/jacsat?d=yIl2AUoC8zA
http://feeds.feedburner.com/~r/acs/jacsat/~4/ND8oy78Fk1s
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[NMR paper] Conformation and Lipid Interaction of the Fusion Peptide of the Paramyxovirus PIV5 in Anionic and Negative-Curvature Membranes From Solid-State NMR.
Conformation and Lipid Interaction of the Fusion Peptide of the Paramyxovirus PIV5 in Anionic and Negative-Curvature Membranes From Solid-State NMR.
http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--pubs.acs.org-images-pubmed-acspubs.jpg Related Articles Conformation and Lipid Interaction of the Fusion Peptide of the Paramyxovirus PIV5 in Anionic and Negative-Curvature Membranes From Solid-State NMR.
J Am Chem Soc. 2014 Jan 16;
Authors: Yao H, Hong M
Abstract
Viral fusion proteins catalyze the merger of the virus...
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01-17-2014 11:07 PM
[NMR paper] The Na(+) transport in Gram-positive bacteria defect in the Mrp antiporter complex measured with (23)Na-NMR.
The Na(+) transport in Gram-positive bacteria defect in the Mrp antiporter complex measured with (23)Na-NMR.
Related Articles The Na(+) transport in Gram-positive bacteria defect in the Mrp antiporter complex measured with (23)Na-NMR.
Anal Biochem. 2013 Oct 15;
Authors: Górecki K, Hägerhäll C, Drakenberg T
Abstract
(23)Na-NMR has previously been used to monitor Na(+) translocation across membranes in Gram-negative bacteria and in various other organelles and liposomes using a membrane-impermeable shift reagent to resolve the signals...
Protocols for Studying the Interaction of MSI-78 with the Membranes of Whole Gram-Positive and Gram-Negative Bacteria by NMR.
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