Related ArticlesProteomics and (1)H NMR-based metabolomics analysis of pathogenic Vibrio vulnificus aquacultures isolated from sewage drains.
Environ Sci Pollut Res Int. 2017 Sep 01;:
Authors: Zhang C, Wang Z, Zhang D, Zhou J, Lu C, Su X, Ding D
Abstract
Vibrio bacteria live in both marine and freshwater habitats and are associated with aquatic animals. Vibrio vulnificus is a pathogenic bacterium that infects people and livestock. It is usually found in offshore waters or within fish and shellfish. This study presents a comparative proteomic analysis of the outer membrane protein (OMP) changes in V. vulnificus proteins after stimulation with sewage from sewage drains. Using two-dimensional electrophoresis followed by MALDI-TOF MS/MS, 32 protein spots with significant differences in abundance were identified and characterized. These identified proteins were found to be involved in various functional categories, including catalysis, transport, membrane proteins progresses, receptor activity, energy metabolism, cytokine activity, and protein metabolism. The mRNA expression levels of 12 differential proteins were further assessed by qRT-PCR. Seven genes including carboxypeptidase, hemoglobin receptor, succinate dehydrogenase iron-sulfur subunit, ATP synthase subunit alpha, thioredoxin, succinyl-CoA synthetase subunit, and alanine dehydrogenase were downregulated upon stimulation, whereas the protein expression levels HupA receptor, type I secretion outer membrane protein, glutamine synthetase, superoxide dismutase, OmpU, and VuuA were upregulated. (1)H NMR spectra showed 18 dysregulated metabolites from V. vulnificus after the sewage stimulation and the pathogenicity was enhanced after that.
PMID: 28864971 [PubMed - as supplied by publisher]
[NMR paper] NMR-based metabolomics reveals the metabolite profiles of Vibrio parahaemolyticus under ferric iron stimulation.
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J Microbiol. 2017 Aug;55(8):628-634
Authors: Zhou J, Lu C, Zhang D, Ma C, Su X
Abstract
Vibrio parahaemolyticus is a halophilic bacterium endemic to coastal areas, and its pathogenicity has caused widespread seafood poisoning. In our previous research, the protein expression of V....
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[NMR paper] Early Effect of Amyloid ?-Peptide on Hippocampal and Serum Metabolism in Rats Studied by an Integrated Method of NMR-Based Metabolomics and ANOVA-Simultaneous Component Analysis.
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http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/https:--images.hindawi.com-linkout-hindawi.button.gif http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/https:--www.ncbi.nlm.nih.gov-corehtml-pmc-pmcgifs-pubmed-pmc.gif Related Articles Early Effect of Amyloid ?-Peptide on Hippocampal and Serum Metabolism in Rats Studied by an Integrated Method of NMR-Based Metabolomics and...
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[NMR paper] (1)H NMR-based metabolomics analysis of adult zebrafish (Danio rerio) after exposure to diniconazole as well as its bioaccumulation behavior.
(1)H NMR-based metabolomics analysis of adult zebrafish (Danio rerio) after exposure to diniconazole as well as its bioaccumulation behavior.
http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--linkinghub.elsevier.com-ihub-images-PubMedLink.gif Related Articles (1)H NMR-based metabolomics analysis of adult zebrafish (Danio rerio) after exposure to diniconazole as well as its bioaccumulation behavior.
Chemosphere. 2017 Feb;168:1571-1577
Authors: Wang Y, Zhu W, Wang D, Teng M, Yan J, Miao J, Zhou Z
Abstract
As a...
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[NMR paper] An Omics Approach to Rational Feed: Enhancing Growth in CHO Cultures with NMR Metabolomics and 2D-DIGE Proteomics.
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J Biotechnol. 2016 Aug 2;
Authors: Blondeel EJ, Ho R, Schulze S, Sokolenko S, Guillemette SR, Slivac I, Durocher Y, Guillemette JG, McConkey BJ, Chang D, Aucoin MG
Abstract
Expression of recombinant proteins exerts stress on cell culture systems, affecting the expression of...
Nuclear Magnetic Resonance (NMR)-Based Metabolomics.
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Methods Mol Biol. 2011;708:321-34
Authors: Keun HC, Athersuch TJ
Biofluids are by far the most commonly studied sample type in metabolic profiling studies, encompassing blood, urine, cerebrospinal fluid, cell culture media and many others. A number of these fluids can be obtained at a high sampling frequency with minimal invasion, permitting detailed characterisation of dynamic metabolic events. One of the attractive properties of...
Proteomics and (1)H NMR-based metabolomics analysis of pathogenic Vibrio vulnificus aquacultures isolated from sewage drains.
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