Related ArticlesProteolytic E-cadherin activation followed by solution NMR and X-ray crystallography.
EMBO J. 2004 Apr 21;23(8):1699-708
Authors: Häussinger D, Ahrens T, Aberle T, Engel J, Stetefeld J, Grzesiek S
Cellular adhesion by classical cadherins depends critically on the exact proteolytic removal of their N-terminal prosequences. In this combined solution NMR and X-ray crystallographic study, the consequences of propeptide cleavage of an epithelial cadherin construct (domains 1 and 2) were followed at atomic level. At low protein concentration, the N-terminal processing induces docking of the tryptophan-2 side-chain into a binding pocket on the same molecule. At high concentration, cleavage induces dimerization (KD=0.72 mM, k(off)=0.7 s(-1)) and concomitant intermolecular exchange of the betaA-strands and the tryptophan-2 side-chains. Thus, the cleavage represents the switch from a nonadhesive to the functional form of cadherin.
[NMR paper] Calcium-dependent homoassociation of E-cadherin by NMR spectroscopy: changes in mobil
Calcium-dependent homoassociation of E-cadherin by NMR spectroscopy: changes in mobility, conformation and mapping of contact regions.
Related Articles Calcium-dependent homoassociation of E-cadherin by NMR spectroscopy: changes in mobility, conformation and mapping of contact regions.
J Mol Biol. 2002 Dec 6;324(4):823-39
Authors: Häussinger D, Ahrens T, Sass HJ, Pertz O, Engel J, Grzesiek S
Cadherins are calcium-dependent cell surface proteins that mediate homophilic cellular adhesion. The calcium-induced oligomerization of the N-terminal two...
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Combined use of computational chemistry, NMR screening, and X-ray crystallography for
Combined use of computational chemistry, NMR screening, and X-ray crystallography for identification and characterization of fluorophilic protein environments.
Related Articles Combined use of computational chemistry, NMR screening, and X-ray crystallography for identification and characterization of fluorophilic protein environments.
Proteins. 2010 Jul 30;
Authors: Vulpetti A, Schiering N, Dalvit C
(19)F NMR screening of fluorinated fragments with different Local Environment of Fluorine, a.k.a. LEF library, is an experimental methodology...
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10-05-2010 12:11 PM
[NMR paper] NMR and crystallography--complementary approaches to structure determination.
NMR and crystallography--complementary approaches to structure determination.
http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--linkinghub.elsevier.com-ihub-images-PubMedLink.gif Related Articles NMR and crystallography--complementary approaches to structure determination.
Trends Biotechnol. 1994 May;12(5):149-53
Authors: MacArthur MW, Driscoll PC, Thornton JM
A knowledge of the three-dimensional structure of a protein is essential to understand how a protein performs its functions. It is also a prime requirement for the rational design...
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08-22-2010 03:33 AM
[NMR paper] NMR and crystallography--complementary approaches to structure determination.
NMR and crystallography--complementary approaches to structure determination.
http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--linkinghub.elsevier.com-ihub-images-PubMedLink.gif Related Articles NMR and crystallography--complementary approaches to structure determination.
Trends Biotechnol. 1994 May;12(5):149-53
Authors: MacArthur MW, Driscoll PC, Thornton JM
A knowledge of the three-dimensional structure of a protein is essential to understand how a protein performs its functions. It is also a prime requirement for the rational design...
nmrlearner
Journal club
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08-22-2010 03:33 AM
[NMR paper] Carbon-13 NMR studies of the lysine side chains of calmodulin and its proteolytic fra
Carbon-13 NMR studies of the lysine side chains of calmodulin and its proteolytic fragments.
Related Articles Carbon-13 NMR studies of the lysine side chains of calmodulin and its proteolytic fragments.
J Protein Chem. 1993 Dec;12(6):695-707
Authors: Huque ME, Vogel HJ
The pH-titration and dynamic behaviour of the seven lysine side chains in bovine calmodulin were studied by carbon-13 NMR. The amino groups of the calcium saturated protein and its proteolytic fragments TR1C (1-75) and TR2C (78-148) were dimethylated with carbon-13 labeled...
Postdoctoral position to study Protein-Protein Interactions and their role in Mechanisms of Signal Transduction using protein solution NMR/x-ray crystallography
Spincore.com are advertising a postdoc NMR position. It sounds pretty interesting.
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Postdoctoral position to study Protein-Protein Interactions and their role in Mechanisms of Signal Transduction using protein solution NMR /x-ray crystallography
Case Medical School, Cleveland, Ohio, USA
How are signaling events transmitted from one protein to another? To answer this question we are looking to add a postdoctoral co-workers to our interdisciplinary team. Our interest is to understand protein-protein interactions, protein structure and dynamics in the context of cell signaling...
Proteolytic E-cadherin activation followed by solution NMR and X-ray crystallography.
Linear Mode
