Related ArticlesProtein stability analysis in ionic liquids by 19F NMR.
Anal Bioanal Chem. 2019 Apr 24;:
Authors: Cheng K, Wu Q, Jiang L, Liu M, Li C
Abstract
Ionic liquids have been extensively used as environmentally friendly solvents for enzymatic reactions and other biological systems. Understanding the mechanism of how ionic liquids affect protein stability is crucial for the biological reaction processes and protein storage using ionic liquids as solvents. Although effects of ionic liquids on protein stability have been studied, equilibrium thermodynamics of protein stability in ionic liquids has not been quantitatively measured. Herein, we utilized 19F NMR to measure the equilibrium thermodynamics of protein stability in ionic liquid [C4-mim]Br. Our results show that proteins are significantly destabilized in [C4-mim]Br ionic liquids. Our results suggest that 19F NMR provides a simple and effective way to study the thermodynamics of protein stability in ionic liquids. 19F NMR will be applicable to facilitate the protein-protein interaction study in ionic liquids. Graphical abstract.
PMID: 31020366 [PubMed - as supplied by publisher]
NMR Studies of Protic Ionic Liquids
From The DNP-NMR Blog:
NMR Studies of Protic Ionic Liquids
Overbeck, Viviane, and Ralf Ludwig. “NMR Studies of Protic Ionic Liquids.” In Annual Reports on NMR Spectroscopy, 95:147–90. Elsevier, 2018.
https://doi.org/10.1016/bs.arnmr.2018.05.002.
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11-25-2018 06:02 AM
Journal Highlight: A review of NMR methods used in the study of the structure and dynamics of ionic liquids
Journal Highlight: A review of NMR methods used in the study of the structure and dynamics of ionic liquids
http://www.spectroscopynow.com/common/images/thumbnails/1613355a2ed.jpgThis mini-review presents a brief research summary of the applications of NMR spectroscopy to the structure and dynamics of pure ionic liquids and their mixtures with lithium salts.
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01-29-2018 01:57 PM
[NMR paper] Characterization of doubly ionic hydrogen bonds in protic ionic liquids by NMR deuteron quadrupole coupling constants - Differences to H-bonds in amides, peptides and proteins.
Characterization of doubly ionic hydrogen bonds in protic ionic liquids by NMR deuteron quadrupole coupling constants - Differences to H-bonds in amides, peptides and proteins.
Related Articles Characterization of doubly ionic hydrogen bonds in protic ionic liquids by NMR deuteron quadrupole coupling constants - Differences to H-bonds in amides, peptides and proteins.
Angew Chem Int Ed Engl. 2017 Sep 17;:
Authors: Ludwig R, Khudozhitkov AE, Stange P, Golub B, Paschek D, Stepanov AG, Kolokolov DI
Abstract
We present the first...
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09-19-2017 04:40 PM
[NMR paper] Characterization of doubly ionic hydrogen bonds in protic ionic liquids by NMR deuteron quadrupole coupling constants - Differences to H-bonds in amides, peptides and proteins
Characterization of doubly ionic hydrogen bonds in protic ionic liquids by NMR deuteron quadrupole coupling constants - Differences to H-bonds in amides, peptides and proteins
We present the first deuteron quadrupole coupling constants (DQCC) for selected protic ionic liquids (PILs) measured by solid-state NMR spectroscopy. The experimental data are supported by dispersion-corrected density functional theory (DFT-D3) calculations and molecular dynamics (MD) simulations. The DQCCs of the N-D bond in the triethylammonium cations are the lowest reported for deuterons in PILs indicating...
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09-18-2017 10:41 AM
Ionic liquids: Enzymatic cellulose processing
Ionic liquids: Enzymatic cellulose processing
http://www.spectroscopynow.com/common/images/thumbnails/1514834b12b.jpgThe promise of room temperature ionic liquids (RTILs) as alternatives to toxic and inflammable volatile organic solvents for green chemistry has been on the boil for at least a couple of decades. Now, team has found that enzymatic activity can be sustained in processing cellulose, from wood, for conversion of this raw material into other useful compounds.
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12-01-2015 10:21 AM
Electron Spin–Lattice Relaxation Mechanisms of Nitroxyl Radicals in Ionic Liquids and Conventional Organic Liquids: Temperature Dependence of a Thermally Activated Process
From The DNP-NMR Blog:
Electron Spin–Lattice Relaxation Mechanisms of Nitroxyl Radicals in Ionic Liquids and Conventional Organic Liquids: Temperature Dependence of a Thermally Activated Process
A detailed understanding of the electron-spin relaxation mechanisms in polarizing agents used for DMP-NMR spectroscopy is crucial for the understanding of the DNP process and to optimize polarizing agents for different DNP applications. The entire study was performed at X-Band frequencies (9 GHz, 14 MHz 1H) and provides many details about the relaxation behavior of nitroxide radicals -...
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07-08-2015 11:11 PM
Post-Doc fellowship in NMR Methods to Study Ionic Solids/liquids - Univ. of Aveiro/Lisbon (Portugal)
Post-Doc fellowship in NMR Methods to Study Ionic Solids/liquids - Univ. of Aveiro/Lisbon (Portugal)
A 1-year postdoctoral position is available starting from January 2011, (extendable for additional 12 months) to study the “solid structure” of ionic liquids (ILs) and ionic solids (ISs) with relevance to the pharmaceutical industry. The research will be centred in the use of NMR techniques in tandem with molecular modelling to study the physical chemistry of molecular interactions in ILs and ISs. The project will be performed at the NMR centres at the Universities of Aveiro (solid state...
Protein stability analysis in ionic liquids by 19F NMR.
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