Publication date: Available online 29 July 2015 Source:International Journal of Biological Macromolecules
Author(s): Dian Xu, Xiangyan Shi, Forrest Thompson, Warner S. Weber, Qiushi Mou, Jeffery L. Yarger
In this study, the secondary structure of the major ampullate silk from Peucetia viridans (Green Lynx) spiders is characterized by X-ray diffraction and solid-state NMR spectroscopy. From X-ray diffraction measurement, ?-sheet nanocrystallites were observed and found to be highly oriented along the fiber axis, with an orientational order, f _ c ? 0.98. The size of the nanocrystallites was determined to be on average 2.5nm x 3.3nm x 3.8nm. Besides a prominent nanocrystalline region, a partially oriented amorphous region was also observed with an f _ a ? 0.89. Two-dimensional 13C-13C through-space and through-bond solid-state NMR experiments were employed to elucidate structure details of Peucetia viridans silk proteins. It reveals that ?-sheet nanocrystallites constitutes 40.0±1.2% of the protein and are dominated by alanine-rich repetitive motifs. Furthermore, based upon the NMR data, 18±1% of alanine, 60±2% glycine and 54±2% serine are incorporated into helical conformations.
[NMR paper] Elucidating proline dynamics in spider dragline silk fibre using 2H-13C HETCOR MAS NMR.
Elucidating proline dynamics in spider dragline silk fibre using 2H-13C HETCOR MAS NMR.
http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--www.rsc.org-images-entities-char_z_RSClogo.gif Related Articles Elucidating proline dynamics in spider dragline silk fibre using 2H-13C HETCOR MAS NMR.
Chem Commun (Camb). 2014 May 14;50(37):4856-9
Authors: Shi X, Yarger JL, Holland GP
Abstract
(2)H-(13)C HETCOR MAS NMR is performed on (2)H/(13)C/(15)N-Pro enriched A. aurantia dragline silk. Proline dynamics are extracted...
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04-22-2015 03:33 PM
[NMR paper] Molecular Dynamics of Spider Dragline Silk Fiber Investigated by 2H MAS NMR.
Molecular Dynamics of Spider Dragline Silk Fiber Investigated by 2H MAS NMR.
http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--pubs.acs.org-images-pubmed-acspubs.jpg Related Articles Molecular Dynamics of Spider Dragline Silk Fiber Investigated by 2H MAS NMR.
Biomacromolecules. 2015 Jan 25;
Authors: Shi X, Holland GP, Yarger JL
Abstract
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[NMR paper] Characterizing the Secondary Protein Structure of Black Widow Dragline Silk Using Solid-State NMR & X-ray Diffraction.
Characterizing the Secondary Protein Structure of Black Widow Dragline Silk Using Solid-State NMR & X-ray Diffraction.
Characterizing the Secondary Protein Structure of Black Widow Dragline Silk Using Solid-State NMR & X-ray Diffraction.
Biomacromolecules. 2013 Sep 11;
Authors: Sampath S, Jenkins JE, Butler E, Kim J, Henning RW, Holland GP, Yarger JL
Abstract
This study provides a detailed secondary structural characterization of major ampullate dragline silk from Latrodectus hesperus (black widow) spiders. X-ray diffraction results show...
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09-13-2013 12:05 PM
[NMR paper] Amino Acid Analysis of spider dragline silk using (1)H NMR.
Amino Acid Analysis of spider dragline silk using (1)H NMR.
Related Articles Amino Acid Analysis of spider dragline silk using (1)H NMR.
Anal Biochem. 2013 May 30;
Authors: Shi X, Holland GP, Yarger JL
Abstract
The amino acid composition of N. clavipes dragline silk fiber is determined by conducting (1)H Nuclear Magnetic Resonance (NMR) spectroscopy experiments on acid hydrolyzed material. N. clavipes dragline silk was found to consist of 43.0±0.6% Gly, 29.3±0.2% Ala, 9.1±0.1% Glx, 4.0±0.1% Leu, 3.3±0.1% Tyr, 3.4 ±0.2% Ser, 2.7±0.1%...
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Elucidating metabolic pathways for amino acid incorporation into dragline spider silk using 13C enrichment and solid state NMR.
Elucidating metabolic pathways for amino acid incorporation into dragline spider silk using 13C enrichment and solid state NMR.
Elucidating metabolic pathways for amino acid incorporation into dragline spider silk using 13C enrichment and solid state NMR.
Comp Biochem Physiol A Mol Integr Physiol. 2011 Jul;159(3):219-24
Authors: Creager MS, Izdebski T, Brooks AE, Lewis RV
Abstract
Spider silk has been evolutionarily optimized for contextual mechanical performance over the last 400 Ma. Despite precisely balanced mechanical properties,...
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09-02-2011 05:40 PM
Solid-state NMR evidence for elastin-like beta-turn structure in spider dragline silk.
Solid-state NMR evidence for elastin-like beta-turn structure in spider dragline silk.
Solid-state NMR evidence for elastin-like beta-turn structure in spider dragline silk.
Chem Commun (Camb). 2010 Sep 28;46(36):6714-6
Authors: Jenkins JE, Creager MS, Butler EB, Lewis RV, Yarger JL, Holland GP
Two-dimensional homo- and heteronuclear solid-state MAS NMR experiments on (13)C/(15)N-proline labeled Argiope aurantia dragline silk provide evidence for an elastin-like beta-turn structure for the repetitive Gly-Pro-Gly-X-X motif prevalent in major...
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12-28-2010 03:31 PM
[NMR paper] NMR characterization of native liquid spider dragline silk from Nephila edulis.
NMR characterization of native liquid spider dragline silk from Nephila edulis.
Related Articles NMR characterization of native liquid spider dragline silk from Nephila edulis.
Biomacromolecules. 2004 May-Jun;5(3):834-9
Authors: Hronska M, van Beek JD, Williamson PT, Vollrath F, Meier BH
Solid spider dragline silk is well-known for its mechanical properties. Nonetheless a detailed picture of the spinning process is lacking. Here we report NMR studies on the liquid silk within the wide sac of the major ampullate (m.a.) gland from the spider...
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11-24-2010 09:51 PM
[NMR paper] Supercontracted spider dragline silk: a solid-state NMR study of the local structure.
Supercontracted spider dragline silk: a solid-state NMR study of the local structure.
http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--linkinghub.elsevier.com-ihub-images-PubMedLink.gif Related Articles Supercontracted spider dragline silk: a solid-state NMR study of the local structure.
Int J Biol Macromol. 1999 Mar-Apr;24(2-3):173-8
Authors: van Beek JD, Kümmerlen J, Vollrath F, Meier BH
The local structure of supercontracted dragline silk from the spider Nephila madagascariensis was investigated by solid-state nuclear magnetic...