NMR paper Protein NMR extends into new fields of structural biology.

		BioNMR > Educational resources > Journal club
[NMR paper] Protein NMR extends into new fields of structural biology.

Webservers

NMR processing:

MDD

NMR assignment:

Backbone:

Side-chains:

NOEs:

Structure from NMR restraints:

Ab initio:

Fragment-based:

Rosetta-NMR (Robetta)

Template-based:

GeNMR

I-TASSER

Refinement:

Amber

Structure from chemical shifts:

Fragment-based:

Homology-based:

Torsion angles from chemical shifts:

Preditor

TALOS

Promega- Proline

Secondary structure from chemical shifts:

CSI (via RCI server)

TALOS

MICS caps, β-turns

d2D

PECAN

Flexibility from chemical shifts:

RCI

Interactions from chemical shifts:

HADDOCK

Chemical shifts re-referencing:

NMR model quality:

NOEs, other restraints:

Chemical shifts:

RDCs:

Pseudocontact shifts:

Protein geomtery:

SAVES2 or SAVES4

Quality Control Check

NMR spectrum prediction:

FANDAS

MestReS

V-NMR

Flexibility from structure:

Backbone S2

Methyl S2

B-factor

Molecular dynamics:

Gromacs

Amber

Antechamber

Chemical shifts prediction:

From structure:

Shiftx2

Sparta+

Camshift

CH3shift- Methyl

ArShift- Aromatic

ShiftS

Proshift

PPM

CheShift-2- Cα

From sequence:

Shifty

Camcoil

Poulsen_rc_CS

Disordered proteins:

MAXOCC

Format conversion & validation:

CCPN

From NMR-STAR 3.1

Validate NMR-STAR 3.1

NMR sample preparation:

Protein disorder:

DisMeta

Protein solubility:

Isotope labeling:

Solid-state NMR:

Thread Tools

Search this Thread

Rate Thread

Display Modes

08-22-2010, 05:08 PM

nmrlearner

Senior Member

Join Date: Jan 2005

Posts: 23,732

Points: 193,617, Level: 100

Level up: 0%, 0 Points needed

Activity: 50.7%

Last Achievements

Award-Showcase

NMR Credits: 0

NMR Points: 193,617

Downloads: 0

Uploads: 0

Protein NMR extends into new fields of structural biology.

Protein NMR extends into new fields of structural biology.

Related Articles Protein NMR extends into new fields of structural biology.

Curr Opin Chem Biol. 1997 Oct;1(3):359-64

Authors: Cooke RM

Advances in protein NMR have opened new doors for the understanding of macromolecular structure and interactions. Isotope-labelling approaches have extended the size limit for structure determination, the mapping of protein-ligand interactions is now widely used and has led to a new drug discovery approach, pathways of protein folding processes can be followed, and the structures of molecules from membranes can be determined.

PMID: 9667871 [PubMed - indexed for MEDLINE]

Source: PubMed

Did you find this post helpful?

Similar Threads
Thread	Thread Starter	Forum	Replies	Last Post
NMR Structural Biology and Biophysics of Protein:Protein Complexes, National Cancer Institute, Frederick, MD, United States NMR Structural Biology and Biophysics of Protein:Protein Complexes, National Cancer Institute, Frederick, MD, United States Postdoctoral positions are available in the Macromolecular NMR section of the Structural Biophysics Laboratory headed by Dr. R. Andrew Byrd at the National Cancer Institute. The Byrd lab combines NMR structural biology & Small Angle Xray Scattering (... More...	nmrlearner	Job marketplace	0	11-15-2011 11:36 PM
Measurement of protein unfolding/refolding kinetics and structural characterization of hidden intermediates by NMR relaxation dispersion [Biophysics and Computational Biology] Measurement of protein unfolding/refolding kinetics and structural characterization of hidden intermediates by NMR relaxation dispersion Meinhold, D. W., Wright, P. E.... Date: 2011-05-31 Detailed understanding of protein function and malfunction hinges on the ability to characterize transiently populated states and the transitions between them. Here, we use 15N, , and 13CO NMR R2 relaxation dispersion to investigate spontaneous unfolding and refolding events of native apomyoglobin. Above pH 5.0, dispersion is dominated by processes involving fluctuations of the F-helix region, which...	nmrlearner	Journal club	0	05-31-2011 11:41 PM
Structural Biology Widget Structural Biology Widget Structural Biology Knowledge Base Widget More...	nmrlearner	General	0	04-15-2011 01:40 AM
Structural biology: Breaking the protein rules - Nature.com (subscription) Structural biology: Breaking the protein rules - Nature.com (subscription) http://nt3.ggpht.com/news/tbn/i9Uh06f_mN2VpM/6.jpg Nature.com (subscription) <img alt="" height="1" width="1" /> Structural biology: Breaking the protein rules Nature.com (subscription) As is Martin Blackledge, an NMR spectroscopist at the Institute of Structural Biology in Grenoble, France, who compares the excitement now to that surrounding the first crystal protein structures in the 1950s. "Every new case is fascinating at the ...	nmrlearner	Online News	0	03-10-2011 04:29 AM
Research Scientist Position in the Cryo-Electron Microscopy Facility at the New York Structural Biol - New York Structural Biology Center - New York, NY, United States Research Scientist Position in the Cryo-Electron Microscopy Facility at the New York Structural Biol - New York Structural Biology Center - New York, NY, United States The New York Structural Biology Center (NYSBC) seeks an experienced electron microscopist to join the staff of its Cryo-Electron Microscope Facility (http://cryoem.nysbc.org). The NYSBC is shared center that supports state-of-the-art research in cryo-EM, NMR, and X-ray. Cryo-EM facilities include four transmission electron microscopes and a new d... More...	nmrlearner	Job marketplace	0	01-26-2011 04:21 AM
Structural Biology Scores With Protein Snapshot Structural Biology Scores With Protein Snapshot (July 12, 2009) -- Investigators have used nuclear magnetic resonance methods to determine the structure of the largest membrane-spanning protein to date. The group's ability to determine the NMR structure of the bacterial protein diacylglycerol kinase, reported in the journal Science, suggests that similar methods can now be used to study the stru More...	nmrlearner	Online News	0	08-19-2010 01:47 PM
NMR in Structural Biology - Alessandro Pintar, Protein Structure and Bioinformatics, ICGEB, Trieste Nuclear Magnetic Resonance in Structural Biology - Alessandro Pintar, Protein Structure and Bioinformatics, ICGEB, Trieste Thanks to advancements both in the theory and in the instrumentation, Nuclear Magnetic Resonance (NMR) has widened its use from small organic molecules to oligosaccharides, peptides, proteins, and nucleic acids. Together with X-ray crystallography, NMR is the only technique that can provide structural information at the atomic level. However, applications of NMR are not limited to 3D structure calculation: it can be used to study flexible biomolecules (peptides,...	MCGowan	Educational web pages	0	09-16-2008 01:50 AM
Fundamentals of Protein NMR Spectroscopy (Focus on Structural Biology) - Gordon S. Rule, T. Kevin Hitchens (2005) Fundamentals of Protein NMR Spectroscopy (Focus on Structural Biology) By Gordon S. Rule & T. Kevin Hitchens (2005) Amazon Book description NMR spectroscopy has proven to be a powerful technique to study the structure and dynamics of biological macromolecules. Fundamentals of Protein NMR Spectroscopy is a comprehensive textbook that guides the reader from a basic understanding of the phenomenological properties of magnetic resonance to the application and interpretation of modern multi-dimensional NMR experiments on 15N/13C-labeled proteins. Beginning with elementary quantum mechanics, a...	JohnW	Books	0	08-19-2008 01:37 PM

« Previous Thread | Next Thread »

Posting Rules
You may not post new threads You may not post replies You may not post attachments You may not edit your posts BB code is On Smilies are On [IMG] code is On HTML code is On Trackbacks are Off Pingbacks are Off Refbacks are Off