NMR paper Protein NMR of biologicals: analytical support for development and marketed products.

		BioNMR > Educational resources > Journal club
[NMR paper] Protein NMR of biologicals: analytical support for development and marketed products.

Webservers

NMR processing:

MDD

NMR assignment:

Backbone:

Side-chains:

NOEs:

Structure from NMR restraints:

Ab initio:

Fragment-based:

Rosetta-NMR (Robetta)

Template-based:

GeNMR

I-TASSER

Refinement:

Amber

Structure from chemical shifts:

Fragment-based:

Homology-based:

Torsion angles from chemical shifts:

Preditor

TALOS

Promega- Proline

Secondary structure from chemical shifts:

CSI (via RCI server)

TALOS

MICS caps, β-turns

d2D

PECAN

Flexibility from chemical shifts:

RCI

Interactions from chemical shifts:

HADDOCK

Chemical shifts re-referencing:

NMR model quality:

NOEs, other restraints:

Chemical shifts:

RDCs:

Pseudocontact shifts:

Protein geomtery:

SAVES2 or SAVES4

Quality Control Check

NMR spectrum prediction:

FANDAS

MestReS

V-NMR

Flexibility from structure:

Backbone S2

Methyl S2

B-factor

Molecular dynamics:

Gromacs

Amber

Antechamber

Chemical shifts prediction:

From structure:

Shiftx2

Sparta+

Camshift

CH3shift- Methyl

ArShift- Aromatic

ShiftS

Proshift

PPM

CheShift-2- Cα

From sequence:

Shifty

Camcoil

Poulsen_rc_CS

Disordered proteins:

MAXOCC

Format conversion & validation:

CCPN

From NMR-STAR 3.1

Validate NMR-STAR 3.1

NMR sample preparation:

Protein disorder:

DisMeta

Protein solubility:

Isotope labeling:

Solid-state NMR:

Thread Tools

Search this Thread

Rate Thread

Display Modes

05-01-2020, 06:13 PM

nmrlearner

Senior Member

Join Date: Jan 2005

Posts: 23,777

Points: 193,617, Level: 100

Level up: 0%, 0 Points needed

Activity: 50.7%

Last Achievements

Award-Showcase

NMR Credits: 0

NMR Points: 193,617

Downloads: 0

Uploads: 0

Protein NMR of biologicals: analytical support for development and marketed products.

Protein NMR of biologicals: analytical support for development and marketed products.

Related Articles Protein NMR of biologicals: analytical support for development and marketed products.

J Biomol NMR. 2020 Apr 29;:

Authors: Casagrande F, Dégardin K, Ross A

Abstract
Application of NMR spectroscopy to derive in-depth characterization of structure and dynamical properties of biomolecules is well established nowadays in many laboratories. Most of these methods rest on the availability of protein labeled with stable isotopes like 13C and 15N. In this report examples are presented on the application of NMR spectroscopic methods to characterize biopharmaceutical proteins in cases no isotope labeled material are available. This is typically found in protein samples used in the development of formulations and production processes. Another important focus of this report is the application of NMR methodology in the field of counterfeit drugs of biologicals and biosimilars. Especially here, NMR does offer relevant structural and quantitative data due to the high versatility of the NMR equipment. An excurse regarding the high medical relevance for a detailed spectroscopic analysis of counterfeits will be presented.

PMID: 32350692 [PubMed - as supplied by publisher]

More...

Did you find this post helpful?

Similar Threads
Thread	Thread Starter	Forum	Replies	Last Post
Protein NMR of biologicals: analytical support for development and marketed products Protein NMR of biologicals: analytical support for development and marketed products Abstract Application of NMR spectroscopy to derive in-depth characterization of structure and dynamical properties of biomolecules is well established nowadays in many laboratories. Most of these methods rest on the availability of protein labeled with stable isotopes like 13C and 15N. In this report examples are presented on the application of NMR spectroscopic methods to characterize biopharmaceutical proteins in cases no isotope labeled material are available....	nmrlearner	Journal club	0	04-29-2020 04:16 PM
Identification of C1q as a Binding Protein for AdvancedGlycation End Products Identification of C1q as a Binding Protein for AdvancedGlycation End Products http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/bichaw/0/bichaw.ahead-of-print/acs.biochem.5b00777/20160113/images/medium/bi-2015-00777m_0009.gif Biochemistry DOI: 10.1021/acs.biochem.5b00777 http://feeds.feedburner.com/~ff/acs/bichaw?d=yIl2AUoC8zA http://feeds.feedburner.com/~r/acs/bichaw/~4/xZPe2pL5Xp0 More...	nmrlearner	Journal club	0	01-14-2016 07:24 AM
Analysis of Moisture, Fat and Protein in Cheese Products - Azom.com Analysis of Moisture, Fat and Protein in Cheese Products - Azom.com <img alt="" height="1" width="1" /> Analysis of Moisture, Fat and Protein in Cheese Products Azom.com The SMART Trac II from CEM enables the testing of moisture and fat using TD-NMR coupled with microwave drying, which has been established as the most accurate rapid method available. The study of the entire sample on a molecular level not just the ... and more » Read here	nmrlearner	Online News	0	03-07-2014 05:41 AM
Cell membrane protein discovery uses NMR spectroscopy - Laboratory Products News Cell membrane protein discovery uses NMR spectroscopy - Laboratory Products News <img alt="" height="1" width="1" /> Cell membrane protein discovery uses NMR spectroscopy Laboratory Products News Guelph, ON - University of Guelph physics professors Vladimir Ladizhansky and Leonid Brown say they have perfected ways to determine the structure of large proteins found in cell membranes throughout the body. Their work may help drug companies and ... and more » Read here	nmrlearner	Online News	0	09-10-2013 08:00 AM
Scientist I, Analytical Development \| Vertex Pharmaceuticals Scientist I, Analytical Development \| Vertex Pharmaceuticals US - Cambridge, MA, Minimum Qualifications- A Ph.D. with 0-3 years of experience in Analytical Chemistry or Pharmaceutical Chemistry. - Skilled in HPLC, LC-MS, GC, GC-MS, FT-IR, UV-VIS, KF, NMR, dissolutionPref More...	nmrlearner	Job marketplace	0	01-05-2013 04:48 PM
Sr. Scientist / Principal Scientist ? Analytical Chemistry ? NMR \| Pfizer Research and Development Sr. Scientist / Principal Scientist ? Analytical Chemistry ? NMR \| Pfizer Research and Development US - Groton, CT, QualificationsTraining & Education:- Doctoral degree in chemistry (analytical or organic) or equivalent with 0-5years of experience in utilization of NMR in Industrial applications or masters degr More...	nmrlearner	Job marketplace	0	07-22-2011 10:39 PM
Industrial Postdoctoral Chemist( analytical, organic or polymer) \| Avomeen Analytical Services Industrial Postdoctoral Chemist( analytical, organic or polymer) \| Avomeen Analytical Services US - Ann Arbor Michigan, Ph.D. in Analytical, organic or polymer chemistry. Hands-on knowledge of following techniques Analytical instrumentation is preferred: FT-IR, NMR, GC, GC-MS, LC-MS, TGA, DSC, ICP, and SEM-EDS. Must be More...	nmrlearner	Job marketplace	0	12-19-2010 01:50 AM
[NMR paper] Interaction of myelin basic protein with single bilayers on a solid support: an NMR, Interaction of myelin basic protein with single bilayers on a solid support: an NMR, DSC and polarized infrared ATR study. Related Articles Interaction of myelin basic protein with single bilayers on a solid support: an NMR, DSC and polarized infrared ATR study. Biochim Biophys Acta. 1993 Sep 19;1151(2):127-36 Authors: Reinl HM, Bayerl TM The interaction of myelin basic protein (MBP) with single bilayers on a solid support (planar and spherical support) is studied by deuterium nuclear magnetic resonance (2H-NMR), differential scanning...	nmrlearner	Journal club	0	08-22-2010 03:01 AM

« Previous Thread | Next Thread »

Posting Rules
You may not post new threads You may not post replies You may not post attachments You may not edit your posts BB code is On Smilies are On [IMG] code is On HTML code is On Trackbacks are Off Pingbacks are Off Refbacks are Off