(1) Background: Prion-like transcellular spreading of tau pathology in Alzheimer's disease (AD) is mediated by tau binding to the cell-surface glycan heparan sulfate (HS). However, the structural determinants for tau-HS interaction are not well understood. (2) Methods and Results: Binding-site mapping using NMR showed two major binding regions in full-length tau responsible for heparin interaction. Thus, two tau constructs, tau PRR2* and tau R2*, were designed to investigate the molecular...
[NMR paper] Automated glycan assembly of 19F labelled glycan probes enables high-throughput NMR studies of protein-glycan interactions
Automated glycan assembly of 19F labelled glycan probes enables high-throughput NMR studies of protein-glycan interactions
Protein-glycan interactions mediate important biological processes, including pathogen host invasion and cellular communication. Major challenges to monitoring these low affinity interactions are the required high sensitivity of a biophysical assay and to cover a breath of synthetic well-defined structures. Here, we showcase an expedite approach that integrates automated glycan assembly (AGA) of 19 F labelled probes and high-throughput NMR methods, enabling the study...
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A Noncanonical Binding Site in the EVH1 Domain ofVasodilator-Stimulated Phosphoprotein Regulates Its Interactions withthe Proline Rich Region of Zyxin
A Noncanonical Binding Site in the EVH1 Domain ofVasodilator-Stimulated Phosphoprotein Regulates Its Interactions withthe Proline Rich Region of Zyxin
http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/bichaw/0/bichaw.ahead-of-print/acs.biochem.7b00618/20170823/images/medium/bi-2017-006182_0008.gif
Biochemistry
DOI: 10.1021/acs.biochem.7b00618
http://feeds.feedburner.com/~ff/acs/bichaw?d=yIl2AUoC8zA
http://feeds.feedburner.com/~r/acs/bichaw/~4/eYP-qgKgrgs
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08-24-2017 08:38 AM
Parkinson's disease protein plays vital 'marshalling' role in healthy brains - Imperial College London
http://www.bionmr.com//t3.gstatic.com/images?q=tbn:ANd9GcTI6lPWDd3Ie9h6B7jOGezFbjXsIUFnj4v26XiDVEgx6oPuj6J0RjppUm1nGnW3GLALRvdvA5oR
Imperial College London
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Parkinson's disease protein plays vital 'marshalling' role in healthy brains
Imperial College London
Using nuclear magnetic resonance spectroscopy, the researchers examined how the protein organised itself structurally in relation to the vesicles. To verify the findings, additional tests were then carried out on samples taken from the brains of rats.
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Parkinson's disease protein...
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Parkinson's Disease protein plays vital â??marshallingâ?? role in healthy brains - Daily News & Analysis
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Daily News & Analysis
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Parkinson's Disease protein plays vital â??marshallingâ?? role in healthy brains
Daily News & Analysis
The study involved lab-based experiments in which synthetic vesicles, modelling the synaptic vesicles found the brain, were exposed to alpha-synuclein. Using nuclear magnetic resonance spectroscopy, the researchers examined how the protein organised ...
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Parkinson's Disease...
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[NMR paper] Multiple cis-trans conformers of the prolactin receptor proline-rich motif (PRM) pept
Multiple cis-trans conformers of the prolactin receptor proline-rich motif (PRM) peptide detected by reverse-phase HPLC, CD and NMR spectroscopy.
http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--www.biochemj.org-images-bj_pubmed.gif http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--www.pubmedcentral.nih.gov-corehtml-pmc-pmcgifs-pubmed-pmc.gif Related Articles Multiple cis-trans conformers of the prolactin receptor proline-rich motif (PRM) peptide detected by reverse-phase HPLC, CD and NMR spectroscopy.
Biochem J. 1996 May 1;315 ( Pt 3):833-44
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[NMR paper] Study of the interaction between salivary proline-rich proteins and a polyphenol by 1
Study of the interaction between salivary proline-rich proteins and a polyphenol by 1H-NMR spectroscopy.
http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--www3.interscience.wiley.com-aboutus-images-wiley_interscience_pubmed_logo_FREE_120x27.gif Related Articles Study of the interaction between salivary proline-rich proteins and a polyphenol by 1H-NMR spectroscopy.
Eur J Biochem. 1994 Feb 1;219(3):923-35
Authors: Murray NJ, Williamson MP, Lilley TH, Haslam E
The interaction between salivary proline-rich proteins and plant polyphenols...
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[NMR paper] Study of the interaction between salivary proline-rich proteins and a polyphenol by 1
Study of the interaction between salivary proline-rich proteins and a polyphenol by 1H-NMR spectroscopy.
http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--www3.interscience.wiley.com-aboutus-images-wiley_interscience_pubmed_logo_FREE_120x27.gif Related Articles Study of the interaction between salivary proline-rich proteins and a polyphenol by 1H-NMR spectroscopy.
Eur J Biochem. 1994 Feb 1;219(3):923-35
Authors: Murray NJ, Williamson MP, Lilley TH, Haslam E
The interaction between salivary proline-rich proteins and plant polyphenols...
Proline-Rich Region II (PRR2) Plays an Important Role in Tau-Glycan Interaction: An NMR Study
Linear Mode
