Proline-directed random-coil chemical shift values as a tool for the NMR assignment o
 

Proline-directed random-coil chemical shift values as a tool for the NMR assignment of the tau phosphorylation sites.

Related Articles Proline-directed random-coil chemical shift values as a tool for the NMR assignment of the tau phosphorylation sites.

Chembiochem. 2004 Jan 3;5(1):73-8

Authors: Lippens G, Wieruszeski JM, Leroy A, Smet C, Sillen A, Buée L, Landrieu I

NMR spectroscopy of the full-length neuronal Tau protein has proved to be difficult due to the length of the protein and the unfavorable amino acid composition. We show that the random-coil chemical shift values and their dependence on the presence of a proline residue in the (i+1) position can successfully be exploited to assign all proline-directed phosphorylation sites. This is a first step toward the study of the phosphorylation of Tau by NMR spectroscopy.

PMID: 14695515 [PubMed - indexed for MEDLINE]



Source: PubMed