NMR paper Projection-reconstruction of three-dimensional NMR spectra.

		BioNMR > Educational resources > Journal club
[NMR paper] Projection-reconstruction of three-dimensional NMR spectra.

Webservers

NMR processing:

MDD

NMR assignment:

Backbone:

Side-chains:

NOEs:

Structure from NMR restraints:

Ab initio:

Fragment-based:

Rosetta-NMR (Robetta)

Template-based:

GeNMR

I-TASSER

Refinement:

Amber

Structure from chemical shifts:

Fragment-based:

Homology-based:

Torsion angles from chemical shifts:

Preditor

TALOS

Promega- Proline

Secondary structure from chemical shifts:

CSI (via RCI server)

TALOS

MICS caps, β-turns

d2D

PECAN

Flexibility from chemical shifts:

RCI

Interactions from chemical shifts:

HADDOCK

Chemical shifts re-referencing:

NMR model quality:

NOEs, other restraints:

Chemical shifts:

RDCs:

Pseudocontact shifts:

Protein geomtery:

SAVES2 or SAVES4

Quality Control Check

NMR spectrum prediction:

FANDAS

MestReS

V-NMR

Flexibility from structure:

Backbone S2

Methyl S2

B-factor

Molecular dynamics:

Gromacs

Amber

Antechamber

Chemical shifts prediction:

From structure:

Shiftx2

Sparta+

Camshift

CH3shift- Methyl

ArShift- Aromatic

ShiftS

Proshift

PPM

CheShift-2- Cα

From sequence:

Shifty

Camcoil

Poulsen_rc_CS

Disordered proteins:

MAXOCC

Format conversion & validation:

CCPN

From NMR-STAR 3.1

Validate NMR-STAR 3.1

NMR sample preparation:

Protein disorder:

DisMeta

Protein solubility:

Isotope labeling:

Solid-state NMR:

Thread Tools

Search this Thread

Rate Thread

Display Modes

11-24-2010, 09:16 PM

nmrlearner

Senior Member

Join Date: Jan 2005

Posts: 23,777

Points: 193,617, Level: 100

Level up: 0%, 0 Points needed

Activity: 50.7%

Last Achievements

Award-Showcase

NMR Credits: 0

NMR Points: 193,617

Downloads: 0

Uploads: 0

Projection-reconstruction of three-dimensional NMR spectra.

Projection-reconstruction of three-dimensional NMR spectra.

Related Articles Projection-reconstruction of three-dimensional NMR spectra.

J Am Chem Soc. 2003 Nov 19;125(46):13958-9

Authors: Kupce E, Freeman R

When three-dimensional NMR spectra are presented as two stereoscopic images, they create a convincing three-dimensional impression for the viewer. In an extension of this principle, we record plane projections of a three-dimensional spectrum at different angles, and use this limited information to reconstruct the entire spectrum. Projections onto different skew planes are derived by Fourier transformation of signals acquired while the two evolution parameters are incremented simultaneously at different rates. By limiting the amount of data gathering, this offers an appreciable economy of instrument time. Normally two to six different projections suffice, depending on the complexity of the spectra. There is an order-of-magnitude speed advantage over the conventional methodology, where both evolution dimensions must be explored independently. Results are presented for reconstructed HNCA and HN(CO)CA spectra of ubiquitin and the HNCO spectrum of a 187-residue protein HasA.

PMID: 14611222 [PubMed - indexed for MEDLINE]

Source: PubMed

Did you find this post helpful?

Similar Threads
Thread	Thread Starter	Forum	Replies	Last Post
Structural Characterisation of a Histone Domain by Projection-Decomposition Structural Characterisation of a Histone Domain by Projection-Decomposition Publication year: 2012 Source: Journal of Magnetic Resonance, Available online 23 February 2012</br> JonasFredriksson, WolfgangBermel, Martin*Billeter</br> We demonstrate that two projection experiments, aN-HSQC–NOESY–N-HSQC and aC-HSQC–NOESY–N-HSQC, recorded for a histone domain from yeast, contain enough information to support a structural characterisation of the protein. At the temperature used, 298K, the histone domain exhibits a very high extent of chemical shift degeneracy that is uncharacteristic for...	nmrlearner	Journal club	0	02-25-2012 04:20 PM
Analysis of non-uniformly sampled spectra with Multi-Dimensional Decomposition Analysis of non-uniformly sampled spectra with Multi-Dimensional Decomposition Publication year: 2011 Source: Progress in Nuclear Magnetic Resonance Spectroscopy, In Press, Accepted Manuscript, Available online 24 February 2011</br> Vladislav Yu., Orekhov , Victor A., Jaravine</br> More...	nmrlearner	Journal club	0	02-26-2011 01:07 PM
[NMR paper] Resolving ambiguities in two-dimensional NMR spectra: the 'TILT' experiment. Resolving ambiguities in two-dimensional NMR spectra: the 'TILT' experiment. Related Articles Resolving ambiguities in two-dimensional NMR spectra: the 'TILT' experiment. J Magn Reson. 2005 Feb;172(2):329-32 Authors: Kupce E, Freeman R Ambiguities in two-dimensional nuclear magnetic resonance spectra due to overlap are usually resolved by recording a three-dimensional version of the experiment. It is shown that a simpler solution is to record a tilted projection of the three-dimensional spectrum, derived by Fourier transformation of the...	nmrlearner	Journal club	0	11-24-2010 11:14 PM
[NMR paper] Projection-reconstruction technique for speeding up multidimensional NMR spectroscopy Projection-reconstruction technique for speeding up multidimensional NMR spectroscopy. Related Articles Projection-reconstruction technique for speeding up multidimensional NMR spectroscopy. J Am Chem Soc. 2004 May 26;126(20):6429-40 Authors: Kupce E, Freeman R The acquisition of multidimensional NMR spectra can be speeded up by a large factor by a projection-reconstruction method related to a technique used in X-ray scanners. The information from a small number of plane projections is used to recreate the full multidimensional spectrum in the...	nmrlearner	Journal club	0	11-24-2010 09:51 PM
[NMR paper] Generalized reconstruction of n-D NMR spectra from multiple projections: application Generalized reconstruction of n-D NMR spectra from multiple projections: application to the 5-D HACACONH spectrum of protein G B1 domain. Related Articles Generalized reconstruction of n-D NMR spectra from multiple projections: application to the 5-D HACACONH spectrum of protein G B1 domain. J Am Chem Soc. 2004 Feb 4;126(4):1000-1 Authors: Coggins BE, Venters RA, Zhou P Reconstructing multidimensional NMR spectra from 2-D projections significantly reduces the time needed for data collection over conventional methodology. Here, we provide a...	nmrlearner	Journal club	0	11-24-2010 09:25 PM
[NMR paper] Reconstruction of the three-dimensional NMR spectrum of a protein from a set of plane Reconstruction of the three-dimensional NMR spectrum of a protein from a set of plane projections. Related Articles Reconstruction of the three-dimensional NMR spectrum of a protein from a set of plane projections. J Biomol NMR. 2003 Dec;27(4):383-7 Authors: Kupce E, Freeman R Three-dimensional protein NMR spectra can be obtained significantly faster than by traditional methods by a projection-reconstruction procedure related to X-ray tomography. First, two orthogonal projections are acquired in quick two-dimensional experiments with the...	nmrlearner	Journal club	0	11-24-2010 09:16 PM
GFT projection NMR spectroscopy for proteins in the solid state. GFT projection NMR spectroscopy for proteins in the solid state. GFT projection NMR spectroscopy for proteins in the solid state. J Biomol NMR. 2010 Oct 30; Authors: Trent Franks W, Atreya HS, Szyperski T, Rienstra CM Recording of four-dimensional (4D) spectra for proteins in the solid state has opened new avenues to obtain virtually complete resonance assignments and three-dimensional (3D) structures of proteins. As in solution state NMR, the sampling of three indirect dimensions leads per se to long minimal measurement time. Furthermore,...	nmrlearner	Journal club	0	11-06-2010 11:02 AM
[NMR paper] Assignment strategies in homonuclear three-dimensional 1H NMR spectra of proteins. Assignment strategies in homonuclear three-dimensional 1H NMR spectra of proteins. Related Articles Assignment strategies in homonuclear three-dimensional 1H NMR spectra of proteins. Biochemistry. 1990 Feb 20;29(7):1829-39 Authors: Vuister GW, Boelens R, Padilla A, Kleywegt GJ, Kaptein R The increase in dimensionality of three-dimensional (3D) NMR greatly enhances the spectral resolution in comparison to 2D NMR. It alleviates the problem of resonance overlap and may extend the range of molecules amenable to structure determination by...	nmrlearner	Journal club	0	08-21-2010 10:48 PM

« Previous Thread | Next Thread »

Posting Rules
You may not post new threads You may not post replies You may not post attachments You may not edit your posts BB code is On Smilies are On [IMG] code is On HTML code is On Trackbacks are Off Pingbacks are Off Refbacks are Off